The comparative study of the antioxidant activity of aqueous and hydroalcoholic extracts of an Iranian endemic species Rhabdosciadium aucheri Boiss.
Subject Areas : Biochemistry
Yasaman
Azimi
1
(Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran)
Seyyedeh Mahdokht
Maddah
2
(Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran)
Golaleh
Mostafavi
3
(Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran)
Keywords: Total phenol, antioxidants, Total flavonoid, methanol extract, Ethanol extract,
Abstract :
Rhabdosciadium aucheri (Apiaceae) is a perennial herbaceous species, which is endemic to W, S and C Iran. In the present study aqueous, ethanolic and methanolic extracts were obtained from aerial parts of the specimens collected from Hamadan province, using both Maceration and Soxhlet methods. Due to the direct relationship between total phenolic and flavonoid contents and the plant antioxidant activity, the aim of the present investigation was the comparative study of total amount of each parameter extracted by aqueous and hydroalcoholic solvents. The results demonstrated that, the highest amount of phenolic and flavonoid compounds extracted by ethanolic solvents using Maceration technique were 37.28 ± 1.03 mg GAE/gr GW and 22.68 ± 0.63 mg QE/gr DW respectively. These results were significant at p < 0.05. Moreover, 72.17 ± 17.10 % was the highest free radical inhibition percentage for methanolic extracts. However, the differences were not considered as significant. The results also showed that using hydroalcoholic solvents are more powerful compared to aqueous solvents in the extraction of phenolic and flavonoid contents and consequently their antioxidant activity.
Ahmed, J., A. Güvenç, N. Küçükboyaci, A. Baldemir and M. Coşkun.2011. 'Total phenolic contents and antioxidant activities of Prangos Lindl.(Umbelliferae) species growing in Konya province (Turkey)'.Turk J Biol,35: 353-360.
Akalın, E. and A. J. N. J. O. B. Akpulat. 2012. 'Rhabdosciadium urusakii sp. nov.(Apiaceae) from east Anatolia (Turkey)'. Turk J Biol, 30:560-564.
Al-Dabbas, M. M. 2017. 'Antioxidant activity of different extracts from the aerial part of Moringa peregrina (Forssk.) Fiori, from Jordan'. Pak J Pharm Sci ,30: 2151-2157.
Amiri, M. S. and M. R. Joharchi.2016. 'Ethnobotanical knowledge of Apiaceae family in Iran: A review'. Avicenna J Phytomed ,6: 621-635.
Christova-Bagdassarian, V., K. S. Bagdassarian, and M. J. I. J. o. A. R. Atanassova. 2013a. 'Phenolic compounds and antioxidant capacity in Bulgarian plans (dry seeds)'.International Journal of Advanced Research, 1, 186-197.
Christova-Bagdassarian, V. L., K. S. Bagdassarian, M. J. M. J. o. P. Atanassova and M. Sciences. 2013b. 'Phenolic profile, antioxidant and antimicrobial activities from the Apiaceae family (dry seeds)'. Mintage Journal of Pharmacy and Medical Science ,2, 26-31.
Christova-Bagdassarian, V. L., K. S. Bagdassarian, M. S. Atanassova and M. A. J. I. H. J. Ahmad .2014. 'Comparative analysis of total phenolic and total flavonoid contents, rutin, tannins and antioxidant capacity in Apiaceae and Lamiaceae families'. Indian Horticulture Journal ,4, 131-140.
Davis, P.,R. Mill and K. J. E. U. P. Tan. 1982. 'Flora of Turkey and the east Aegean ıslands'. 7, 322-323.
Downie, S. R.,D. S. Katz-Downie and M. F. Watson.2000.' A phylogeny of the flowering plant family Apiaceae based on chloroplast DNA rpl16 and rpoC1 intron sequences: towards a suprageneric classification of subfamily Apioideae'. Am J Bot 87, 273-92.
Duran, A., B. Doğan, H. Duman, E. Martin, M. Öztürk and Ö. J. B. Çetin. 2010. 'Taxonomic studies on the genus Rhabdosciadium (Apiaceae) with particular reference to Turkish species and their relationships with some closely related genera'. Biologia, 65 (3): 451-458.
Eghtesadi, F., M. Mehrdad and A. Sonboli. 2006. 'Essential oil composition of Rhabdosciadium aucheri from Iran. Chemistry of Natural Compounds, 42 (4): 489-490.
Firat, M. and Y. J. P.GÜZEL. 2019. 'Contributions to the taxonomy of the Irano-Turanian genus Rhabdosciadium (Apiaceae): Nomenclatural notes, carpology, molecular phylogeny and the description of a new species from Bitlis (Turkey)'. Phytotaxa, 395 (3): 179-198.
Ghribia, L.,H. Ghouilaa,A. Omrib, M. Besbesb and H. B. Janneta. 2014.' Antioxidant and anti-acetylcholinesterase activities of extracts and secondary metabolites from Acacia cyanophylla'. Asian Pac J Trop Biomed 4, S417-23.
Jung, C., V. Maeder, F. Funk, B. Frey, H. Sticher, E. Frossard. 2003. 'Release of phenols from Lupinus albus L. roots exposed to Cu and their possible role in Cu detoxification'. Plant and Soil, 252(2): 301-312.
Kalvandi, R.,M. Mirza, K. Safikhani, M. Naderi, M. .2013. 'Essential oil composition of Rhabdosciadium aucheri Boiss. from Iran'. Journal of Medicinal and Aromatic Plants, 29 (3): 605-610 (In Persian with an abstract in English) 29.
Kaska, A. and R. Mammadov. 2019.' Antioxidant properties, proximate content and cytotoxic activity of Echinophora tournefortii Jaub. & Spach Food Science and Technology. Food Science and Technology, 39 (4): 875.
Khazai, V., K. Piri, S. Nazeri, R. Karamian and N. Zamani. 2011.' Free radical scavenging activity and phenolic and flavonoid contents of Echinophora platyloba DC'. Asian Journal of Medical and Pharmaceutical Researches, 1(1): 09-11
Köroglu, A., M. M. Hürkul and Ö. Özbay.2012. 'Antioxidant Capacity and Total Phenol Contents of Bifora radians Bieb'. FABAD Journal of Pharmaceutical Sciences, 37 (3): 123-127
Lefahal, M., N. Zaabat, R. Ayad, E. H. Makhloufi, L. Djarri, M. Benahmed, H. Laouer, G. Nieto and S. Akkal. 2018. 'In Vitro Assessment of Total Phenolic and Flavonoid Contents, Antioxidant and Photoprotective Activities of Crude Methanolic Extract of Aerial Parts of Capnophyllum peregrinum (L.) Lange (Apiaceae) Growing in Algeria'. Medicines, 5 (26): 1-10.
Lyskov, D., E. Kljuykov, E.D. GÜNER and T. Samigullin. 2017.' Molecular phylogeny of the genus Rhabdosciadium (Apiaceae) with description of a new species R. anatolyi from Hakkâri province, eastern Turkey'. Phytotaxa, 331(2): 253-262.
Martins, N., L. Barros, C. Santos-Buelga and I. Ferreira.2016. 'Antioxidant potential of two Apiaceae plant extracts: A comparative study focused on the phenolic composition. Industrial Crops and Products, 79: 188-194.
Matejić, J., A. Džamić, T. Mihajilov-Krstev, V. Ranđelović and P. Marin. 2015. 'Antioxidant and antimicrobial potential of Opopanax hispidus (Apiaceae) extracts'. Lekovite Sirovine, 35: 141-150.
Mazandarani, M., S. Makari, G. R. Bajian, M. P. Zarghami and M. Abrodi. 2012. 'Evaluation of phytochemical and antioxidant activitiy in different parts of Heracleum gorganicum Rech. F. in Golestan Province of Iran. Iranian Journal of Plant Physiology.2 (2): 381-386.
Mileski, K., A. M. Džamić, A. Ćirić, S. Grujić, M. Ristić, V. Matevski and P. Marin. 2014.' Radical scavenging and antimicrobial activity of essential oil and extracts of Echinophora sibthorpiana Guss. from Macedonia.' Archives of Biological Sciences 66 (1): 401-413.
Pirbalouti, A. G., M. Setayesh, A. Siahpoosh and H. Mashayekhi. 2013. 'Antioxidant activity, total phenolic and flavonoids contents of three herbs used as condiments and additives in pickles products'. Herba Polonica, 59 (3): 51-62.
Saleem, F. 2010. 'Anti-diabetic potentials of phenolic enriched Chilean potato and select herbs of Apiaceae and Lamiaceae Families'. M.Sc. thesis, University of Massachusetts, Amherst, Massachusetts, USA.
Senthilkumar, R.,T. Parimelazhagan,O. Chaurasia and R. Srivastava. 2013. 'Free radical scavenging property and antiproliferative activity of Rhodiola imbricata Edgew extracts in HT-29 human colon cancer cells'. Asian Pacific Journal of Tropical Medicine, 6 (1): 11-19.
Yassa, N., H. R. Beni and A. Hadjiakhoondi. 2008. 'Free radical scavenging and lipid peroxidation activity of the Shahani black grape'. Pakistan Journal of Biological Sciences, 11 (21): 2513-2516.