Reduction of Phytotoxic Effect of Cadmium Heavy Metal by Biomass of Edible Fungus, Armillaria tabescens
Subject Areas : Stress Physiology
1 - Department of Biology, Faculty of Science, Atatürk University Turkey
Keywords: Hydroponic, Mushroom, Metal, heavy, edible,
Abstract :
In this study, it is aimed to reduce the damage caused by Cd, one of the heavy metals, on the corn plant with the biomass of the edible mushroom Armillaria tabescens. For this purpose; Control, Cd application (0.1 mM), Cd + A. tabescens (0.01 mM + 10g biomass) application and A.tabescens (10g biomass) application were prepared as the experimental setup. Our study was carried out in a hydroponic environment. Firstly root-stem lengths and wet-dry weight were determined. Lipid peroxidation (LPO) and reactive oxygen species (H2O2 and O2-) and antioxidant enzyme (SOD) levels were measured. In addition, changes in protein, chlorophyll, proline and sugar levels in seedlings were also evaluated. According to our results, in hydroponics medium, the damage size were high in the corn plants exposed to Cd heavy metal and the damage was low in Cd + A. tabescens application. In addition, it was seen that A. tabescens significantly reduced heavy metal stress in Cd + A.tabescens application by considering the changes in chlorophyll content, carotoneid, total sugar, protein and proline content. Moreover, it was observed that only A. tabescens application contributed to the growth and development of corn plant. Our research findings show that A. tabescens mushrooms can be used to reduce Cd stress. This study is a first in terms of heavy metal removal in hydroponic environment.
ABD Allah E.F., H. Abeer, A. A. Alqarawi and A. Alwathnani Hend. 2015. 'Alleviation of adverse impact of cadmium stress in sunflower (Helianthus annuus L.) by arbuscular mycorrhizal fungi'. Pak. J. Bot, 47(2), 785-795.
Agarwal, S. and V. Pandey. 2004.' Antioxidant enzyme responses to NaCl stress in Cassia angustifolia'. Biologia Plantarum, 48(4), 555-560.
Amir, H., A. Lagrange, N. Hassaïne and Y. Cavaloc. 2013.' Arbuscular mycorrhizal fungi from New Caledonian ultramafic soils improve tolerance to nickel of endemic plant species'. Mycorrhiza, 23(7), 585-595.
Bates LS., RP Waldren and ID Teare . 1973. 'Rapid determination of free proline for water-stress studies', Plant and Soil, 39, 205-207.
Becher M., IN. Talke, L. Krall and U. Kramer .2004. 'Cross-species microarray transcript profiling reveals high constitutive expression of metal homeostasis genes in shoots of the zinc hyperaccumulator Arabidopsis halleri', Plant J, 37, 251–268.
Bhaduri, A. M. and M.H. Fulekar. 2012. 'Assessment of arbuscular mycorrhizal fungi on the phytoremediation potential of Ipomoea aquatica on cadmium uptake'. Biotech, 2(3), 193-198.
Burritt, D. J. 2012. 'Proline and the cryopreservation of plant tissues: functions and practical applications'. In Current frontiers in cryopreservation. IntechOpen.
Clemens, S. 2006. 'Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants'. Biochimie, 88(11), 1707-1719.
DalCorso, G., A. Manara and A. Furini. 2013.' An overview of heavy metal challenge in plants: from roots to shoots'. Metallomics, 5(9), 1117-1132.
Dische, Z. 1962. 'Color reactions of pentoses'. Methods in carbohydrate chemistry, 1, 484-488.
Durkan, N., I. Ugulu, M.C. Unver, Y. Dogan and S. Baslar. 2011. 'Concentrations of trace elements aluminum, boron, cobalt and tin in various wild edible mushroom species from Buyuk Menderes River Basin of Turkey by ICP-OES'. Trace Elements and Electrolytes, 28(4), 242.
Gill SS. and N. Tuteja. 2010. 'Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants'. Plant Physiol Biochem 48, 909–930.
Gill, S. S., N. A. Khan and N. Tuteja.2012. 'Cadmium at high dose perturbs growth, photosynthesis and nitrogen metabolism while at low dose it up regulates sulfur assimilation and antioxidant machinery in garden cress (Lepidium sativum L.)'. Plant Science, 182, 112-120.
Hassan, S. E., M. Hijri and M. St-Arnaud. 2013. 'Effect of arbuscular mycorrhizal fungi on trace metal uptake by sunflower plants grown on cadmium contaminated soil'. New biotechnology, 30(6), 780-787.
Irfan, M., A. Ahmad and S. Hayat. 2014. 'Effect of cadmium on the growth and antioxidant enzymes in two varieties of Brassica juncea'. Saudi journal of biological sciences, 21(2), 125-131.
Jaleel, C.A., B. Sankar, R. Sridharan and R. Panneerselvam. 2008. 'Soil salinity alters growth, chlorophyll content, and secondary metabolite accumulation in Catharanthus roseus'. Turk. J. Biol., (32), 79–83.
Jin, H. Q., H. B. Liu, Y. Y. Xie, Y. G. Zhang, Q. Q. Xu, L.J. Mao, L. J., ... and C. L. Zhang. 2018. 'Effect of the dark septate endophytic fungus Acrocalymma vagum on heavy metal content in tobacco leaves'. Symbiosis, 74(2), 89-95.
Latef, A. A. 2018. 'Growth and some physiological activities of pepper (Capsicum annuum L.) in response to cadmium stress and mycorrhizal symbiosis'. Sciences, India Section B: Biological Sciences, 87(2), 571-578.
Lebeau, T., A. Braud and K. Jézéquel. 2008. 'Performance of bioaugmentation-assisted phytoextraction applied to metal contaminated soils: a review'. Environmental Pollution, 153(3), 497-522.
Li, X., Y. Wang, Y. Pan, H. Yu, X. Zhang, Y. Shen and S. Zhang. 2017. 'Mechanisms of Cd and Cr removal and tolerance by macrofungus Pleurotus ostreatus HAU-2'. Journal of hazardous materials, 330, 1-8.
Ling-Zhi, L. I. U., G. O. N. G. Zong-Qiang, Y. L. Zhang and L. I. Pei-Jun. 2011. 'Growth, cadmium accumulation and physiology of marigold (Tagetes erecta L.) as affected byarbuscular mycorrhizal fungi. Pedosphere, 21(3), 319-327.
Márquez, S. S.,G. F. Bills, L.D. Acuña and I. Zabalgogeazcoa. 2010. 'Endophytic mycobiota of leaves and roots of the grass Holcus lanatus'. Fungal Diversity, 41(1), 115-123.
Miransari, M. 2011. 'Hyperaccumulators, arbuscular mycorrhizal fungi and stress of heavy metals'. Biotechnology Advances, 29(6), 645-653.
Nakano, Y., and K. Asada. 1981.' Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts'. Plant and cell physiology, 22(5), 867-880.
Nongmaithem, N., A. Roy and P. M. Bhattacharya. 2017. 'Potential of Trichoderma spp. on Growth Promotion and Mitigating Cadmium Uptake in Rice Plant under the Metal Stress Ecosystem'. Int. J. Curr. Microbiol. App. Sci, 6(6), 992-1010.
Sajedi, N. A., M. R. Ardakani,F. Rejali, F. Mohabbati and M. Miransari. 2010. 'Yield and yield components of hybrid corn (Zea mays L.) as affected by mycorrhizal symbiosis and zinc sulfate under drought stress'. Physiology and Molecular Biology of Plants, 16(4), 343-351.
Smith, M. A. and P.J. Davies. 1985.' Separation and quantitation of polyamines in plant tissue by high performance liquid chromatography of their dansyl derivatives'. Plant Physiology, 78(1), 89-91.
Trombini TL, E. Oliveira and D. B. Berlese . 2015. 'Inhibition of thiolcontaining enzymes in erythrocytes of workers exposed to lead'. Interciencia 40(2):84–90
Velikova, V., T. Tsonev, A. Edreva, A. Gürel and H. Hakerlerler.2002. 'Effects of reddening of cotton (Gossypium hirsutum L.) leaves on functional activity of photosynthetic apparatus. Photosynthetica, 40(3), 449-452.
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|
Velikova, V., I. Yordanov and A. Edreva. 2000. 'Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines'. Plant science, 151(1), 59-66.
Wang, J. L., T. Li, T.,G. Y. Liu, J. M. Smith and Z. W. Zhao. 2016. 'Unraveling the role of dark septate endophyte (DSE) colonizing maize (Zea mays) under cadmium stress: physiological, cytological and genic aspects. Scientific reports, 6, 22028.
Witham, F. H. and D. F. Blaydes. 1971. 'Experiments in plant physiology' (No. 581.1 W5).Published in 1971 in New York NY
Zhang, H. H., M. Tang, H. Chen, C. L. Zheng and Z. C. Niu. 2010. 'Effect of inoculation with AM fungi on lead uptake, translocation and stress alleviation of Zea mays L. seedlings planting in soil with increasing lead concentrations'. European Journal of Soil Biology, 46(5), 306-311.
Zheng, W., F. E. I. Yingheng and Y. Huang. 2009. 'Soluble protein and acid phosphatase exuded by ectomycorrhizal fungi and seedlings in response to excessive Cu and Cd'. Journal of Environmental Sciences, 21(12), 1667-1672.