Effect of Different Fat Sources on some Blood Metabolites, Hormones, and Enzyme Activities of Lambs with Different Residual Feed Intake in Heat-Stressed Condition

Subject Areas : Camel

G.  Halakoo ¹ (Department of Animal Science, Faculty of Agricultural Science, Sari Agricultural Science and Natural Resources University, Sari, Iran)
ا.  تیموری یانسری ² (Department of Animal Science, Animal Science and Aquaculture faculty, Agricultural and Natural Resource University, Sari, Mazandaran, Iran.)
M.  Mohajer ³ (Department of Animal Science, Golestan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Gorgan, Iran)
Y.  Chashnidel ⁴ (Department of Animal Science, Faculty of Agricultural Science, Sari Agricultural Science and Natural Resources University, Sari, Iran)

Received: 2019-10-30 Accepted : 2020-02-29 Published : 2020-12-01

Keywords: heat stress, lamb, Fat, residual feed intake,

Abstract :

Most efficient animals in heat-stressed condition, intelligently regulate their metabolism for maximizing their productivity and fats play an important role in reducing heat stress in these animals but the underlying mechanisms remain elusive. The effects of different fat sources on some blood metabolites and hormones and enzyme activities of heat-stressed lambs with different residual feed intake (RFI) were studied. In the preliminary trial, 48 male lambs of four different breeds (Zel, Dalagh, and their hybrids with Romanov; BW=16.87±0.55 kg) in a block randomized complete design used to assay RFI for 67 days. After that, 32 lambs of the same breeds as a block (BW=30.74±1.21 kg) with two different RFIs in heat stressed condition were used in a 2 × 4 factorial trail over 84 days. The treatments included four rations: 1) basal ration (control), 2, 3, and 4) rations supplemented with calcium soap fatty acids (FA), beef tallow, and canola oil, respectively. Dry matter intake (DMI) was high in control and high RFI groups (low efficient) (p < 0.05). Lipid sources had significant effect on serum glucose, cholesterol, triiodothyronine (T3), and Insulin, pulse and respiration rate (p < 0.05). No differences found between treatments for triglyceride, thyroxine(T4), low-density lipoprotein (LDL), and Very-low-density lipoprotein (VLDL). Glucose concentration had strong correlation with the RFI (p < 0.01). The Low RFI lambs (LRFI) had high T3 and low LDH levels. These lambs had high respiration and pulse rate (p < 0.05). Lambs fed with fat supplemented rations had higher concentration of serum aspartate aminotransferase (AST), alkaline phosphatase (ALP), and lactic dehydrogenase (LDH) enzymes than control (p < 0.01). Lipid supplementation in heat stressed lambs markedly alters glucose, cholesterol, T3 and respiration and pulse rate that independently of reduced dry matter intake (DMI) through coordinated changes in fuel supply and utilization by multiple tissues. Even more challenging the most efficient lambs (LRFI) had high physiologic rate and high activity for removing heat from tissues.

References:

Allen M.S. and Bradford B.J. (2012). Control of food intake by metabolism offuels – a comparison across species. Proc. Nutr. Soc. 71, 401-409.

AOAC. (1995). Official Methods of Analysis. 12^th Ed. Association of Official Analytical Chemists, Arlington, Washington, DC., USA.

Arthur P.F., Archer J.A., Johnston D.J., Herd R.M., Richardson E.C. and Parnell P.F. (2001). Genetic and phenotypic variance and covariance components for feed intake, feed efficiency, and other postweaning traits in Angus cattle. J. Anim. Sci. 79, 2805-2811.

Bhatt S., Gething P.W., Brady O.J., Messina J.P., Farlow A.W. and Moyes C.L. (2013). The global distribution and burden of dengue. Nature. 496, 504-507.

Baumgard L.H. and Rhoads Jr R.P. (2013). Effects of heat stress on postabsorptive metabolism and energetics. Annu. Rev. Anim. Biosci. 1, 311-337.

Becú-Villalobos D., García-Tornadú I., Shroeder G., Salado E.E., Gagliostro G., Delavaud C., Chilliard Y. and Lacau-Mengido I.M. (2007). Effect of fat supplementation on leptin, insulin-like growth factor I, growth hormone, and insulin in cattle. Canadian j. Vet. Res. 71, 218-225.

Belhadj Slimen I., Najar T., Ghram A., Abdrrabba M.J.J. and Nutrition A. (2016). Heat stress effects on livestock: Molecular, cellular and metabolic aspects, a review. Anim. Physiol. Anim. Nutr. 100, 401-412.

Bernabucci U., Lacetera N., Baumgard L.H., Rhoads R.P., Ronchi B. and Nardone A. (2010). Metabolic and hormonal acclimation to heat stress in domesticated ruminants. Animal 4, 1167-1183.

Bianchi A.E., Macedo V.P., França R.T., Lopes S.T., Lopes L.S., Stefani L.M., Volpato A., Lima H.L., Paiano D. and Machado G. (2014). Effect of adding palm oil to the diet of dairy sheep on milk production and composition, function of liver and kidney, and the concentration of cholesterol, triglycerides and progesterone in blood serum. Small Rumin. Res. 117, 78-83.

Block S., Smith J., Ehrhardt R., Diaz M., Rhoads R., Van Amburgh M. and Boisclair Y. (2003). Nutritional and developmental regulation of plasma leptin in dairy cattle1. J. Dairy Sci. 86, 3206-3214.

Buchet R., Millán J.L. and Magne D. (2013). Multisystemic functions of alkaline phosphatases. Pp. 27-51 in Phosphatase Modulators. J. Millan, Ed. Humana Press, Totowa, New Jersey.

Carabano M.J., Logar B., Bormann J., Minet J., Vanrobays M.L., Díaz C., Tychon B., Gengler N. and Hammami H. (2016). Modeling heat stress under different environmental conditions. J. Dairy Sci. 99, 3798-3814.

Dechow C., Baumrucker C., Bruckmaier R. and Blum J. (2017). Blood plasma traits associated with genetic merit for feed utilization in Holstein cows. J. Dairy Sci. 100, 8232-8238.

Faure J., Lefaucheur L., Bonhomme N., Ecolan P., Meteau K., Coustard S.M., Kouba M., Gilbert H. and Lebret B.J.M. (2013). Consequences of divergent selection for residual feed intake in pigs on muscle energy metabolism and meat quality. Meat Sci. 93, 37-45.

Flay H., Kuhn-Sherlock B., Macdonald K., Camara M., Lopez-Villalobos N., Donaghy D. and Roche J. (2019). Hot topic: Selecting cattle for low residual feed intake did not affect daily methane production but increased methane yield. J. Dairy Sci. 102(3), 2708-2713.

Gillis M., Duckett S., Sackmann J., Realini C., Keisler D. and Pringle T. (2004). Effects of supplemental rumen-protected conjugated linoleic acid or linoleic acid on feedlot performance, carcass quality, and leptin concentrations in beef cattle. J. Anim. Sci. 82, 851-859.

Gonzalez-Rivas P.A., Chauhan S.S., Ha M., Fegan N., Dunshea F.R. and Warner R.D. (2019). Effects of heat stress on animal physiology, metabolism, and meat quality: A review. Meat Sci. 162, 8-25.

Haddad S. and Younis H.M. (2004). The effect of adding ruminally protected fat in fattening diets on nutrient intake, digestibility and growth performance of Awassi lambs. Anim. Feed Sci. Technol. 113(1), 61-69.

Haraki H.M.C., Gandra J.R., Oliveira E.R., Takiya C.S., Goes R.H.T.B., Gabriel A.M.A., Rodrigues G.C.G., Gandra E.R.S., Pereira T.L., Damiani J. and Batista J.D.O. (2018). Effects of chitosan and whole raw soybeans on feeding behavior and heat losses of Jersey heifers. Iranian J. Appl. Anim. Sci. 8(3), 397-405.

Herd R.M., Velazco J.I., Smith H., Arthur P.F., Hine B., Oddy H., Dobos R.C. and Hegarty R.S. (2019). Genetic variation in residual feed intake is associated with body composition, behaviour, rumen, heat production, haematology and immune competence traits in Angus cattle. J. Anim. Sci. 97(5), 2202-2219.

Iranian Council of Animal Care. (1995). Guide to the Care and Use of Experimental Animals. Isfahan University of Technology Isfahan, Isfahan, Iran.

Kaneko J.J., Harvey J.W. and Bruss M.L. (2008). Clinical Biochemistry of Domestic Animals. Academic Press, San Diego, California.

Kellner T., Baumgard L., Prusa K., Gabler N. and Patience J. (2016). Does heat stress alter the pig’s response to dietary fat? J. Anim. Sci. 94, 4688-4703.

Koch R.M., Swiger L.A., Chambers D. and Gregory K. (1963). Efficiency of feed use in beef cattle. J. Anim. Sci. 22, 486-494.

Koppe S.W., Elias M.S., Moseley R.H. and Green R.M. (2009). Trans-fat feeding results in higher serum alanine aminotransferase and increased insulin resistance compared to a standard murine high-fat diet. Am. J. Physiol. Gastrointest. Liver Physiol. 297(2), 378-384.

Liao C.C., Lin Y.L. and Kuo C.F. (2015). Effect of high-fat diet on hepatic proteomics of hamsters. J. Agric. Food Chem. 63, 1869-1881.

Lkhagvadorj S. (2010). Effects of selection for low residual feed intake and feed restriction on gene expression profiles and thyroid axis in pigs. MS Thesis. Iowa State University, Iowa, USA.

Loor J., Ferlay A., Ollier A., Doreau M. and Chilliard Y. (2005). Relationship among trans and conjugated fatty acids and bovine milk fat yield due to dietary concentrate and linseed oil. J. Dairy Sc. 88, 726-740.

Min L., Zheng N., Zhao S., Cheng J., Yang Y., Zhang Y., Yang H., Wang J.J.B. and communications, b. r. (2016). Long-term heat stress induces the inflammatory response in dairy cows revealed by plasma proteome analysis. Biochem. Biophys. Res. Commun. 471, 296-302.

Morera P., Hosoda K. and Bernabucci U. (2012). Chronic heat stress up-regulates leptin and adiponectin secretion and expression and improves leptin, adiponectin and insulin sensitivity in mice. J. Mol. Endocrinol. 48(2), 129-138.

NRC. (1971). A Guide to Environmental Research on Animals. 9^th Ed. National Academy Press, Washington, DC, USA.

NRC. (2001). Nutrient Requirements of Dairy Cattle. 7^th Ed. National Academy Press, Washington, DC, USA.

O’Brien R., Rhoads P., Sanders S.R., Duff G.C. and Baumgard L.H. (2010). Metabolic adaptations to heat stress in growing cattle. Domest. Anim. Endocrinol. 38, 86-94.

Palmquist D. and Jenkins T. (2017). A 100-year review: fat feeding of dairy cows. J. Dairy Sci. 100, 10061-10077.

Parvar R., Ghoorchi T. and Shargh M. S. J. S. R. R. (2017). Influence of dietary oils on performance, blood metabolites, purine derivatives, cellulase activity and muscle fatty acid composition in fattening lambs. Int. J. Mol. Sci. 150, 22-29.

Pinho R.A., Sepa-Kishi D.M., Bikopoulos G., Wu M.V., Uthayakumar A., Mohasses A., Hughes M.C., Perry C.G., Ceddia R. B. J. F. r. b., and medicine (2017). High-fat diet induces skeletal muscle oxidative stress in a fiber type-dependent manner in rats. Free Radic. Biol. Med. 110, 381-389.

Rabiee A., Breinhild K., Scott W., Golder H., Block E. and Lean I. J. J. o. d. s. (2012). Effect of fat additions to diets of dairy cattle on milk production and components: A meta-analysis and meta-regression. J. Dairy Sci. 95, 3225-3247.

Rajion M.A., McLean J.G. and Cahill R.N. (1985). Essential fatty acid metabolism in the fetal and neonatal lamb. Australian J. Biol. Sci. 38, 33-40.

Rincon-Delgado R., Gutierrez-Banuelos H. and Perez-Vasquez E. J. A. J. (2011). Relationship of residual feed intake on specific hematological and biochemical parameters in Rambouillet sheep. Agric. J. 6, 87-91.

Sadeghi A.A. and Moghaddam M. (2018). The effects of turmeric, cinnamon, ginger and garlic powder nutrition on antioxidant enzymes’ status and hormones involved in energy metabolism of broilers during heat stress. Iranian J. Appl. Anim. Sci. 8(1), 125-130.

Saeed M., Abbas G., Alagawany M., Kamboh A.A., El-Hack M.E.A., Khafaga A.F. and Chao S.J. (2019). Heat stress management in poultry farms: A comprehensive overview. J. Therm. Biol. 84, 414-425.

SAS Institute. (2013). SAS^®/STAT Software, Release 9.4. SAS Institute, Inc., Cary, NC. USA.

Shwartz G., Rhoads M., VanBaale M., Rhoads R. and Baumgard L. (2009). Effects of a supplemental yeast culture on heat-stressed lactating Holstein cows. J. Dairy Sci. 92, 935-942.

Tanaka K. (2005). Occurrence of conjugated linoleic acid in ruminant products and its physiological functions. Anim. Sci. J. 76, 291-303.

Toufighi K., Ahangari Y.J., Rouzbehan Y. and Karimi K. (2014). Effect of rumen-protected fat on performance and serum concentration of T3 hormone of Holstein young bulls during hot, humid weather. Italian J. Anim. Sci. 13, 3202-3210.

Van Soest P., Robertson J. and Lewis B. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74, 3583-3597.

Wheelock J., Rhoads R., VanBaale M., Sanders S. and Baumgard L. (2010). Effects of heat stress on energetic metabolism in lactating Holstein cows. J. Dairy Sci. 93, 644-655.