Effects of Pre-Treatment with GnRH on the Efficiency of Superstimulatory Protocol in Water Buffalo (Bubalus bubalis)

Subject Areas : Camel

S. Singhal ¹ , S. Prasad ² , H. Singh ³ , M. Shukla ⁴ , J.K. Prasad ⁵

1 - Department of Animal Reproduction, Gynaecology and Obstetrics, G.B. Pant University of Agriculture and Technology, Pantnagar, India
2 - Reproduction, Gynaecology and Obstetrics, G.B. Pant University of Agriculture and Technology, Pantnagar, India
3 - Regional, Centre, Lala Lajpat Rai University of Veterinary and Animal Science, Karnal, India
4 - Department of Animal Reproduction, Gynaecology and Obstetrics, G.B. Pant University of Agriculture and Technology, Pantnagar, India
5 - Department of Animal Reproduction, Gynaecology and Obstetrics, G.B. Pant University of Agriculture and Technology, Pantnagar, India

Received: 2021-06-03 Accepted : 2021-06-03 Published : 2021-06-01

Keywords: Embryo, GnRH, Buffalo, follicle stimulating hormone,

Abstract :

The multiple ovulation embryo transfer technique (MOET) in buffalo has yielded poor superovulation response due to presence of large/dominant follicle(s) at the start of superstimulatory treatment. The aim of present study was to evaluate the effects of pretreatment with gonadotropin on number of total as well as viable and transferrable embryos recovered during MOET programme in Murrah buffaloes. Buffaloes (n=27) enrolled into two treatment (groups A and B, n=9 in each group) and one control (group C, n=9) groups were administered with 600 mg of follicle stimulating hormone (FSH) in 10 decreasing doses at 12 hourly interval for five days. Additionally, buffaloes were administered with a single dose of GnRH in group A (10 µg) and group B (06 µg) as a pre-treatment on 2.5 day prior to the start of FSH. Two doses of prostaglandin (500 µg) were administered with the 7th and 8th dose of FSH in all the buffaloes. Embryos were collected non-surgically on day 5.5 post-insemination. Number of total, transferrable and the non-transferrable embryos recovered were recorded in the 3 groups. Total as well as the viable transferrable number of embryos recovered were significantly higher (p <0.05) in the buffaloes of group B compared to the control (3.0 vs. 1.33 and 2.33 vs. 1.0, respectively). In all the three groups, about 75% of embryos recovered were of transferrable grade. The results of study indicated that administration of GnRH as a pre-treatment in superstimulatory treatment improved the MOET efficiency in buffalo.

References:

Alapati A., Kapa S.R., Jeepalyam S., Rangappa S.M. and Yemireddy K.R. (2010). Development of the body condition score system in Murrah buffaloes: Validation through ultrasonic assessment of body fat reserves. J. Vet. Sci. 11, 1-8.

Angela S., Carolina D.C., Floriana D.R., Alberto P., Gianluigi Z., Gianluca N. and Bianca G. (2018). Evaluation of factors involved in the failure of ovum capture in superovulated buffaloes. Theriogenology. 122, 102-108.

Baruselli P.S., Soares J.G., Bayeux B.M., Silva J.C.B., Mingoti R.D. and Carvalho N.A.T. (2018). Assisted reproduction technologies (ART) in water buffaloes. Anim. Reprod. 15(1), 971- 983.

Baruselli P.S., Soares J.G., Gimenes L.U., Monteiro B.M., Olazarri M.J. and Carvalho N.A.T. (2013). Control of buffalo follicular dynamics for artificial insemination, superovulation and in vitro embryo production. Buffalo Bull. 32, 160-176. 

Bulbul B., Kirbas M., Dursun S. and Kose M. (2013). Superovulation in cows synchronized with two different progesterone+oestradiol protocols. Arch. Tierz. 55, 161-169.

Bulbul B., Kirbas M., Kose M., Dursun S. and Colak M. (2010). Investigation of superovulation response in cows by manipulation of the oestrus cycle using progesterone and oestradiol, Kafkas Üniv. Vet. Fak. Derg. 16(3), 463-468.

Bungartz L. and Niemann H. (1993). Effects of a dominant follicle on ovarian responses of dairy cows following various superovulatory treatment schedules. Theriogenology. 39, 198-204.

Carvalho N.A.T., Nichi M., Gallupo A.G., Melo G.M., Souza R.J., DAngelo M. and Baruselli P.S. (2006). Oviduct ciliary currents in bovine and buffalo at day 1after ovulation. J. Reprod. Fertil. Dev. 18, 287-288.

Driancourt M.A., Fry R.C., Clarke I.J. and Cahill L.P. (1987). Follicular growth and regression during the 8 days after hypophysectomy in sheep. J. Reprod. Fertil. 79, 635-644.

Heleil B. and El-Deeb Y. (2010). Superovulatory response in relation to follicular dynamics and presence of dominant follicles in Egyptian buffaloes. Adv. Biol. Res. 4, 169-174. 

Hesheng J., Yingming W., Quinyang J., Jingwei W., Zhijian W., Peijun W., Bingkun X., Haipeng H. and Xiao H. (2006). Pregnancies resulted from transfer of in vivo embryos derived from nonsurgical recovery in superovulated buffaloes. Pp. 53-58 in Proc. 5^th Asian Buffalo Congr., Nanning, China.

Honparkhe M., Gandotra V.K., Matharoo J.S., Ghuman S.P.S., Dadarwal D. and Singh J. (2014). Synchronization of follicular wave emergence following ultrasound guided transvaginal follicle ablation or estradiol-17β administration in water buffalo (Bubalus bubalis). Anim. Reprod. Sci. 146(1), 5-14.

Kandil O.M., Abdoon A.S.S. and Kacheva D. (2012). Successful embryo transfer in Egyptian buffaloes. Glob. Vet. 8, 320-327.

Karaivanov C., Kacheva D., Petrov M., Vlahov K. and Sapundjiev E. (1990). Superovulatory response of river buffalo (Bubalus bubalis). Theriogenology. 33, 453-464.

Kohram H. and Poorhamdollah M. (2012). Relationships between the ovarian status and superovulatory responses in dairy cattle. Anim. Reprod. Sci. 131, 123-128.

Leonardo P.S., Diego D.M., Aldemar C.R., Juan G.J. and Diego V.A. (2019). Superovulatory response in Brahman donor cows using follicular ablation before superovulation protocols. Rev. MVZ Cordoba. 24, 7203-7208.

Macmillan K.L. and Thatcher W.W. (1991). Effects of an agonist of gonadotropin-releasing hormone on ovarian follicles in cattle. Biol. Reprod. 45, 883-889.

Misra A.K. and Tyagi S. (2007). In vivo embryoproduction in buffalo: current situation and future perspectives. Italian J. Anim. Sci. 6, 74-91.

Misra A.K., Kasiraj R., Mutha Rao M., Rangareddy N.S., Jaiswal R.S. and Pant H.C. (1998). Rate of transport and development of preimplantation embryo in the superovulated buffalo (Bubalus bubalis). Theriogenology. 50, 637-649.

Misra A.K., Mutha R.M., Kasiraj R., Rangareddy N.S. and Pant H.C. (1999). Bull specific effect on fertilization rate and viable embryo recovery in the superovulated buffalo (Bubalus bubalis). Theriogenology. 52, 701-707.

Mohammadi A., Seifi H.A. and Farzaneh N. (2019). Effect of prostaglandin F_2α and GnRH administration at the time of artificial insemination on reproductive performance of dairy cows. Vet. Res. Forum. 10, 153-158.

Nanda A.S., Brar P.S. and Prabhakar S. (2003). Enhancing reproductive performance in dairy buffalo, major constraints and achievements. Reproduction. 61, 27-36.

Naseer Z., Ahmad N., Khan M.I.R., Ahmad E., Tahir M.Z. and Singh J. (2012). Effect of GnRH and estradiol benzoate on follicular wave emergence, estrus, ovulation and pregnancy rate in CIDR treated Nilli Ravi buffaloes. J. Anim. Plant Sci. 22, 142-146.

Neglia G., Gasparrini B., Vecchio D., Di Palo R., Zicarelli L. and Campanile G. (2010). Progesterone supplementation during multiple ovulation treatment in buffalo species (Bubalus bubalis). Trop. Anim. Health Prod. 42, 1243-1247.

Phillips P.E. and Jahnke M.M. (2016). Embryo transfer (techniques, donors and recipients). Vet. Clin. Food Anim. 32, 365-385.

Phogat J.B., Pandey A.K. and Singh I. (2016). Seasonality in buffalo reproduction. Int. J. Plant Anim. Environ. Sci. 6, 46-55.

Pursley J.R., Mee M.O. and Wiltbank M.C. (1995). Synchronization of ovulation in dairy cows using PGF_2α and GnRH. Theriogenology. 44, 915-923.

Ravi S.K., Prasad S., Singh B., Prasad J.K. and Singhal S. (2011). Hormonal profile in superovulated buffaloes following ablation of dominant follicle. Indian Vet. J. 88, 25-27.

Redhead A.K., Siewb N., Lambie N., Carnarvon D., Ramgattie R. and Knights M. (2018). The relationship between circulating concentration of AMH and LH content in the follicle stimulating hormone (FSH) preparations on follicular growth and ovulatory response to superovulation in water buffaloes. Anim. Reprod. Sci. 188, 66-73.

Salvador I., Cebrian-Serrano A., Salamone D. and Silvestre M.A. (2011). Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine. Spanish J. Agric. Res. 9, 744-752.

Sato T., Nakada K., Uchiyama Y., Kimura Y., Fujiwara N., Sato Y., Umeda M. and Furukawa T. (2005). The effect of pretreatment with different doses of GnRH to synchronize follicular wave on superstimulation of follicular growth in dairy cattle. J. Reprod. Dev. 51, 512-515.

Singhal S. (2010). Hormonal manipulation of dominant follicle and its effect on superovulation in buffaloes. Ph D. Thesis. G.B. Pant Universityof Agriculture and Technology, Pantnagar, India.

SPSS Inc. (2011). Statistical Package for Social Sciences Study. SPSS for Windows, Version 20. Chicago SPSS Inc., USA.

Techakumphu M., Sukavong Y., Intaramongkol S. and Intaramongkol J. (2001). The effect of gonadotropin releasing hormone on superovulation in elite swamp buffalo cows (Bubalus bubalis). J. Vet. Med. Sci. 63, 853-857.

Ulker H., Kanter M., Gokdal O., de Avila D.M. and Reeves J.J. (2001). The effects of recombinant LHRH fusion proteins on testicular development and histology in ram lambs. Deutsche Dtsch. Tierärztl. Wochenschr. 108, 459-464.

Ullah N., Wright Jr R.W., Mehmood A. and Baig S.M. (1992). Endocrine profile in relation to ovarian response, recovery rate and quality of embryos in Nili-Ravi buffaloes treated with FSH. Buffalo J. 1, 47-56.

Veldhuis J.D., O'Dea L.S. and Johnson M.L. (1989). The nature of the gonadotropin-releasing hormone stimulus-luteinizing hormone secretary response of human gonadotrophs in vivo. J. Clin. Endocrinol. Metab. 68, 661-670.

Wilson J.M., Jones A.L. and Miller D.R. (1990). Influence of a dominant follicle on the superovulatory response. Theriogenology. 33, 349-357.