Effect of Goat Follicular Fluid on in vitro Production of Embryos in Black Bengal Goats

Subject Areas : Camel

S.A. Masudul Hoque ¹ , M.A.M. Yahia Khandoker ² , S.K. Kabiraj ³ , L.Y. Asad ⁴ , M. Fakruzzaman ⁵ , K.M.A. Tareq ⁶

1 - Department of Animal Breeding and Genetics, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh
2 - Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh
3 - Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh
4 - Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh
5 - Department of Animal Breeding and Genetics, Patuakhali Science and TechnologyUniversity, Barisal, 8210, Bangladesh
6 - Patuakhali Science and Technology University, Patuakhali, 8210, Bangladesh

Received: 2011-07-11 Accepted : 2011-10-21 Published : 2012-09-01

Keywords: black bengal goat, goat follicular fluid, invitro culture, <i>in vitro</i> fertilization, <i>in vitro</i> maturation,

Abstract :

The study was undertaken to elucidatethe beneficial effects of goat follicular fluid (gFF) added to maturation and culture media on in vitro maturation, fertilization and post-fertilization development of oocytes in Black Bengal goats. Follicular fluid and oocytes were collected from slaughter house goat’s ovaries using the aspiration technique. Media were prepared using TCM-199 supplemented with 0.5% bovine serum albomin (BSA) plus four levels of gFF at concentrations of 0%, 5%, 10% and 15%. Oocytes were matured for 27 h, fertilized with capacitated fresh semen in Brackett and Oliphant (BO) medium for 6 h and then cultured up to 7 days, at 38.5 ˚C with 5% CO2 under humidified air. It was observed with 0% to 15% of gFF that 53.8-75.0% of the oocytes reached the cumulus cell expansion level-3; 41.5-67.8% reached metaphase-II; 28.6-38.4% exhibited normal fertilization (formation of 2-pronuclei); 12.3-33.7% were 2-cell embryos. The development of embryos was arrested at the 2-cell stage in control media and at the 8-cell stage in 5% media, though morula and blastocyst stages developed in 10% (14.1% and 9.3%, respectively) and 15% gFF media (18.0% and 10.0%, respectively). In comparison, it was observed that the results in all stages of in vitro production of embryos could be significantly increased (P<0.05) by adding 5% gFF to control media. The results could further be improved (P<0.05) by increasing the level of gFF to 10% but no further increment (P>0.05) occurred when gFF increased to 15% level. It is concluded that gFF has a positive effect on in vitro production of embryos in Black Bengal goats and a 10% level of gFF is recommended based on the improvements observed and the associated economic benefits.

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