Effects of Dietary Supplement of Organic Acids Induced Protective Immunity against Coccidiosis

Subject Areas : Camel

آ.ی.  عبدوالهی ¹ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China|Department of Animal Science, Kano University of Science and Technology Wudil, PMB3244, Kano, Nigeria)
ایکس.جی.  یو ² (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
ی.کیو.  فو ³ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
م.و.  وانگ ⁴ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
ن.اس.  کی ⁵ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
م.اچ.  گیزا ⁶ (College of Animal Science, South China Agricultural University, Guangzhou, China)
اس.  کالون ⁷ (Department of Animal Science, Njala University, Freetown, Sierra Leone|College of Animal Science, South China Agricultural University, Guangzhou, China)
و.د.  پان ⁸ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
ایکس.ل.  شی ⁹ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
ی.  فانگ ¹⁰ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
اس.  ویو ¹¹ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
و.  هو ¹² (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
ل.پی.  تان ¹³ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
کا.ایکس.  لی ¹⁴ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
ز.  وانگ ¹⁵ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
جی.جی.  زو ¹⁶ (College of Animal Science, South China Agricultural University, Guangzhou, China)
د.ی.  فنگ ¹⁷ (College of Animal Science, South China Agricultural University, Guangzhou, China)
جی.کیو.  لی ¹⁸ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)

Received: 2019-02-01 Accepted : 2019-05-15 Published : 2020-03-01

Keywords: immunity, cytokines, organic acid, Coccidiosis, T-cells,

Abstract :

This study evaluated effects of three products of dietary organic acids (Acidomix® AFG; Activate® AD and Lacplus) on protective immunity against coccidiosis. Two hundred and eighty 1-day broiler chickens were randomly divided into 8 groups by 5 replications (7 birds per replicate), comprising 2 controls (negative=N; positive=P) and 6 supplemented organic acid groups (1% AFG, 1% DA, 1% Lacplus; 2% AFG, 2% DA and 2% Lacplus). With the exception of N control (uninfected), all 7 groups were orally challenged with 8 × 105 sporulated oocysts of Eimeria tenella at day 12 post-hatch. Samples of blood, spleen and ceca were collected on day 22 and 35 post-hatch. Body weight gain, fecal oocyst shedding, lesion score, mortality rate and pH of the ceca were assessed. ELISA was used to detect Cluster of T cell differentiation (CD4+, CD8+) molecules and reverse transcription polymerase chain reaction (RT-PCR) was employed for detection of pro-inflammatory chemokine (IFNγ-) and cytokines expression (IL-8, IL-15 and IL-17). The result revealed that supplementation of organic acids significantly increased body weight gain, improved feed conversion ratio (FCR), reduced lesion scores and oocyst index (p <0.05) compared to P control (infected non-supplemented). The mortality rate was higher in P control. The pH of ceca was significant (p <0.05) and maximum was observed in P control. Chicks fed 2% DA and 1% Lacplus had greater percentages of CD4 T-cell molecules, but had decreased CD8 T cell molecules, suggesting a protective function of these T-cell subsets in innate immune response against E. tenella infection. The cecal and splenic chemokine and cytokines mRNA expression encoding IFN-γ, IL-8, IL-15 and IL-17 showed higher levels of transcript compared to N and P controls, indicating the organic acid products might have exerted their protective effects by improving their production. It is concluded that the Activate® DA at 2% and Lacplus 1% levels at both 22 and 35-days post-hatch have shown best anticoccidial effects.

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