Evaluation the application of gibberellic acid hormone in improvement of wheat × maize crosses in chromosome elimination method
Subject Areas :Hamed Modirrousta 1 , Raheleh Khademian 2 , Reza Bozorgipour 3 , Ali Saremirad 4
1 - Graduated Master Department of Genetic and plant breeding faculty of Agriculture and Natural Resources, Imam Khomeini, International University, Qazvin, Iran
2 - Asistant Professor of Department of Genetic and plant breeding faculty of Agriculture and Natural Resources, Imam Khomeini, International University, Qazvin, Iran
3 - Associate Professor Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
4 - Ph. D. student, Department of Agronomy and Plant Breeding, Young Researchers and Elite Club, Karaj Branch, Islamic Azad University, Karaj, Iran
Keywords: Maize, Wheat, Hormonal treatment, Interspecific hybridization, Haploid,
Abstract :
Haploid plants because of this, they are noteworthy, which shorten the time to reach pure lines. This study aimed to evaluate the effect of hormone use gibberellic acid hormone as well as determine the optimal time took for produce embryo in the chromosome elimination method. In this study, of six F2 genotypes product from bread wheat crosses which as a female parent were used to obtain wheat haploid embryos. Gibberellic acid hormone was sprayed on spike pollinated as well as stems and leaves. The application gibberellic acid was sprayed after lapse 2 hours, 2 days, 4 days, 6 days, 8 days and 10 days from the pollination. The maximum seed yield was obtained from genotype 3 with 76.09% and gibberellic acid spray treatment in 2 hours after pollination with 85.09%. The maximum haploid embryo was obtained of genotype 2 with 8.32% and Gibberellic acid hormone spray was obtained in 6 days after pollination with %6.99. Among the treatments, none of them showed a significant increase in compared to the control. The results showed that the use of gibberellic acid hormone played a role in increased number seed and embryo size.
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