Callus induction and regeneration of bread wheat cultivars and barley from mature embryo explants
Subject Areas :Ali Akbar Gholami 1 , Alireza Tarinejad 2
1 - Tabriz, Maragheh- Tabriz, 35 kilometers of roads, Azerbaijan Shahid Madani University, Faculty of Agriculture, Department of Agricultural Biotechnology
2 - Tabriz, Maragheh- Tabriz, 35 kilometers of roads, Azerbaijan Shahid Madani University, Faculty of Agriculture, Department of Agricultural Biotechnology
Keywords: barley, Wheat, mature embryo, callus and regeneration,
Abstract :
In this research, the ability of callus and regeneration of four wheat lines, CD-4, CD-6, CD-8, CD-9 and three barley cultivars (Afzal, Valfajr and Kavir) from adult fetal specimens Evaluated. For wheat callus induction, ML1G1 medium containing three levels of growth regulator 2, 4-D was used to regenerate the ML1R3 medium containing three levels of Kin and BAP growth regulators. For callus induction, ML1G1 medium containing three levels of growth regulator 2, 4-D was used for regeneration of ML1R3 medium containing different levels of BAP and Kin growth regulator. The callus evaluation of different wheat varieties showed that the highest amount of callus related to the CD-9 (79.55%) line and also at different levels of the Thorrod growth regulator had the highest level of callus induction 2.4 mg / L( 77/58%). Evaluation of regeneration of wheat cultivars showed that the highest reproductive rate related to CD-9 line (20.66%), as well as in different culture media, had the highest regeneration rate of 2 mg / L Kin Kinseed medium (27.22% ) Was. The results of callus evaluation of different barley varieties showed that the highest amount of callus induction related to Afzal cultivar (65.33%), as well as at different levels of Growth regulator, had the highest level of callus induction related to 3.5 mg / L (66 / 50%). The highest regeneration rate of Afzal cultivar (13.55%) and also in different culture media had the highest reproduction rate of 2 mg / L BAP (20%).
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