Chitinase production from a native isolate of Beauveria bassiana

Subject Areas : Environmental pollutions (water, soil and air)

Masoumeh Kordi ¹ , Elnaz Fahimi ² , Nasser Farrokhi ³

1 - PhD student in Agricultural Biotechnology, Department of Plant Sciences and Biotechnology, Faculty of Life Sciences & Biotechnology, Shahid Beheshti Unvirsity, Iran.
2 - MSc in Agricultural Biotechnology, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood Unvirsity of Technology, Iran.
3 - Assistant Professor at Department of Cell & Molecular Biology, Faculty of Life Sciences & Biotechnology, Shahid Beheshti Unvirsity, Iran. *(Corresponding Author)

Received: 2016-01-04 Accepted : 2016-08-14 Published : 2018-06-22

Keywords: Beauveria spp, Chitin, chitinase enzyme, biological control,

Abstract :

Biological control is a suitable substitute technique for chemical battle with pests. Enthomopathogenic fungus Beauveria spp. attacks its target host by penetrating into cuticle through secretion of hydrolytic enzymes such as chitinases. Chitinases are capable of hydrolyzing chitins with many other applications in biocontrol, sea bioremediation, and development of biopesticides, pharmaceuticals. In this research, chitinase enzyme activities from Beauveria bassiana fungus, cultured in 3 culture media, were measured through a colorimetric assay. A native isolate with high mortality rate isolated and identified from Minoodasht. The highest chitinase activity was achieved at day 3 when incubated at 50 ˚C in Pontecorvo medium containing 0.75% colloidal chitin (pH = 7). Our results might be useful for higher production of chitinase that can be implemented in biological control and further elevate human health and environmental biosafety.  

References:

1-      Kim, J. S. & Je, Y. H. 2010. A novel biopesticide production: attagel-mediated precipitation of chitinase from Beauveria bassiana SFB-205 supernatant for thermotolerance. Applied Microbiology and Biotechnology, VOL. 87, PP. 1639-1648.

2-      Pathan, A. A., Devi, K. U., Vogel, H. & Peineke, A. 2007. Analysis of differential gene expression in the generalist entomopathogenic fungus Beauveria bassiana (Bals.) Vuillemin grown on different insect cuticular extracts and synthetic medium through cDNA-AFLPs. Fungal Genetics and Biology, VOL. 44, PP. 1231-1241.

3-      Martin-Gil, F., Leal, J. A., Gomez-Miranda, B., Martin-Gil, J., Prieto, A. and Ramos-Sanchez, M. C. 1992. Low temperature thermal behaviour of chitins and chitin-glucans. Thermochimica Acta, VOL. 211, PP. 241-254.

4-      De León, A., Jimenez-islas, H., Gonzalez-cuevas, M. & Rosa, A. P. 2004. Analysis of the expression of the Trichoderma harzianum ech42 gene in two isogenic clones of Escherichia coli by surface response methodology. Process Biochemistry, VOL. 39, PP. 2173-2178.

5-      Raaijmakers, J. M., Leeman, M. & Oorschot, V. 1995. Dose-response relationships in biological control of Fusarium wilt of radish by Pseudomonas spp. Phytopathology, VOL. 85(10), PP. 1075-1081.

6-      Bierbaum, S., Nickel, R., Koch, A., Lau, S., Deichmann, K., Wahn, U., Superti-Furga, A. & Heinzmann, A. 2005. Polymorphisms and haplotypes of acid mammalian chitinase are associated with bronchial asthma. American Journal of Respiratory and Critical Care Medicine, VOL. 172, PP. 1505-1512.

7-      Binod, P., Pusztahelyi, T., Nagy, V., Sandhya, C., Szakacs, G., Pocsi, I. & Pandey, A. 2005. Production and purification of extracellular chitinases fromPenicilliumaculeatum NRRL 2129 under solid-state fermentation. Enzyme and Microbial Technology, VOL. 36, PP. 880-887.

8-      Smith, R. J. & Grula, E. A. 1986. Chitinase is an inducible enzyme in Beauveria bassiana. Journal of Invertebrate Pathology, VOL. 42, PP. 319-326.

9-      Coudron, T. A., Kroha, M. J. & Ignoffo, C. M. 1984. Levels of chitinolytic activity during development of three entomopathogenic fungi. Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, VOL. 3, PP. 339-348.

10-  Havukkala, I., Mitamura, C., Hara, S., Hirayae, K., Nishizawa, Y. & Hibi, T. 1993. Induction and Purification of Beauveria bassiana Chitinolytic Enzymes. Plos pathogens, VOL. 61, PP. 97-102.

11-  Suresh, P. V. & Chandrasekaran, M. 1998. Utilization of prawn waste for chitinase production by the marine fungus Beauveria bassiana by solid state fermentation. World Journal of Microbiology & Biotechnology, VOL.14, PP. 655-660.

12-  Patidar, P., Agrawal, D., Banerjee, T. & Patil, S. 2005. Chitinase production by Beauveria felina RD 101: optimization of parameters under solid substrate fermentation conditions. World Journal of Microbiology & Biotechnology, VOL. 21, PP. 93-95.

13-  13-Pontecorvo, G. 1953. The genetic of Aspergillus nidulans. Department of Genetics, the university, Glasgow Scotland.Advances in Genetics, VOL. 5, PP. 141–239.

14-  14-Roberts, W. K. & Selitrennikoff, C. P. 1998. Plant and bacterial chitinases differ in antifungal activity. Journal of General Microbiology, VOL. 134, PP. 426-428.

15-  Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein -dye binding. Analytical Biochemistry, VOL. 72, PP. 248-252.

16-  Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing suger. Analytical Chemistry, VOL. 3, PP. 426-428.

17-  17-Leopold, J. & Samsinakova, A. 1970. Quantitative estimation of chitinase and Severalother enzymes in the fungus Beuuveria bassiana. Journal of Invertebrate pathology, VOL. 15, PP. 34-42.

18-  18-Coudron, T. A., Kroha, M. J. & Ignoffo, C. M. 1984. Levels of chitinolytic activity during development of three entomopathogenic fungi. Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, VOL. 3, PP. 339-348.