Production of chitosan from shrimp producted chitin with two acids and determination degree of acetylation by FT-IR spectroscopy
Subject Areas : Agriculture and EnvironmentMasoumeh Kordi 1 , Naser Farrokhi 2
1 - - PhD student in Agricultural Biotechnology, Department of Plant Sciences and Biotechnology, Faculty of Life Sciences & Biotechnology, Shahid Beheshti University, Tehran, Iran.
2 - Assistant Professor at Department of Cell & Molecular Biology, Faculty of Life Sciences & Biotechnology, Shahid Beheshti University, Tehran, Iran.
Keywords: Chitosan, acetylation degree, Chitin, ftir,
Abstract :
Production of chitosan from shrimp producted chitin with two acids and determination degree of acetylation by FT-IR spectroscopyAbstract:After cellulose, chitin found in nature in the shells of crustaceans, exoskeleton of insects, nematodes, and fungi cell wall in complex forms with other proteins and polysacharids. Chitosan produced by deacetylation of chitin and used in medico until agriculture. The chitin with different percentages of industrial acid was prepared from shrimp shells and functional properties of chitosan prepared from these samples by Fourier transform (FTIR) was studied. FTIR pattern almost matched samples with standard samples. The preparation of chitosan film and its IR spectrum showed that the degree of acetylation of chitosan obtained between 22-18 liable by Brugnerotto and coworker (2001) method. In view of shrimp abondance in northern and southern parts of the country, The commissioning a very precious product line is the possibile that economic growth and replacing it with chemicals incompatible with the environment in solving the environmental problems of human societies is the most effective.Keywords: Chitin, Chitosan, FTIR, Acetylation degree.
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- Garcia Alonos, I., Peniche-Covas, C., Nieto, J.M., 1983. Determination of The Degree of Acetylation of Chitin and Chitosan by Thermal analysis. Journal of Thermal Analysis and Calorimetry, Vol. 28, pp.189-193.
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- Dutta, P.K., Dutta, J., Tripathi, V., 2004. Chitin and chitosan: chemistry, properties and applications. Journal of Scientific & Industrial Research, Vol. 63, pp.20-31.
- Ilium, L., 1998. Chitosan and its use as a pharmaceutical excipient. Pharmaceutical Research, Vol. 15, pp.1326–1331.
- Chien, P.J., Sheu, F., Yang, F.H., 2007. Effects of edible chitosan coating on quality and shelf life of sliced mango fruit. Journal of Food Engineering, Vol. 78, pp.225–229.
- Ramnani, S.P., Sabharwal, S., 2006. Adsorption behavior of Cr(VI) onto radiation cross linked chitosan and its possible application for the treatment of wastewater containing Cr(VI). Reactive and Functional Polymers, Vol. 66, pp.902–909.
- Mao, H.Q., Roy, K., Troung-Le, V.L., Janes, K.A., Line, K.Y., Wang, Y., 2002. Chitosan-DNA nanoparticles as gene carriers: Synthesis, characterization and transfection efficiency. Journal of Controlled Release, Vol. 70, pp.399–421.
- Alder, E., 1997. Chitin natural macromolecules. Chem. of Macromolecules. Internet. Pdf. http://www.seaborne.com/chitinguide.htm. p.10.
- Pagel, D., 1999. Chitin Production from Lobster and Crab in: NatlAcdemy Press, Island., pp. 61-68.
- Takiguchi, Y., 1991. Chitosan-Jikken Manual, Advances in chitin science, Proceedings from the Asia-Pacific. In: Chen R.H. and Chen H. C. (ed) Physical properties of chitinous material Chitin, Gihodou Shupan Kabushki Kaisha, Japan, pp.1-7.
- Chang, K.L., Tsai, G., 1997. Response surface optimization and kinetics of isolating chitin from pink shrimp (Solenocera melantho) shell waste. J. Agric. Food Chemistry, Vol. 45, pp.1900-1904.
- Brugnerotto, J., lizard, J., Goycoolea, F.M., Monal, W.A., Desbrieres, J., Rinaudo, M., 2001. An infrared investigation in relation with chitin and chitosan characterization. Polymer, Vol. 42, pp. 3569-3580.
- Garcia Alonos, I., Peniche-Covas, C., Nieto, J.M., 1983. Determination of The Degree of Acetylation of Chitin and Chitosan by Thermal analysis. Journal of Thermal Analysis and Calorimetry, Vol. 28, pp.189-193.
- Heu, M.S., Kim, J.S., Shahidi, F., 2003. Components and nutritional quality of shrimp processing by products. Food Chemistry, Vol. 82, pp.235-242.
- Beaney, P., Mendoza, J.L., Healy, M., 2005. Comparison of chitins produced by chemical and bioprocessing methods. Journal of Chemical Technology & Biotechnology, Vol. 80, pp.145-150.