The effect of six weeks aerobic training and cinnamon extract on resistin gene expression in liver tissue of male rats feeded by high fat diet
Subject Areas : Cellular & Molecular Exercise Biology and Sports Genetic
Roghaye Hosseini
1
,
tahere bagherpoor
2
*
,
Nematolah Nemati
3
1 - Department of Sport Sciences, Da. C., Islamic Azad University, Damghan, Iran.
2 - Department of Sport Sciences, Da. C., Islamic Azad University, Damghan, Iran.
3 - Department of Sport Sciences, Da. C., Islamic Azad University, Damghan, Iran.
Keywords: Aerobic Exercise, Resistin Gene, Liver Tissue, Body Weight, High-fat Diet, Obesity,
Abstract :
Increased expression of the resistin gene in the liver causes insulin resistance, and in rats lacking this gene, fasting glucose levels decrease. Resistin inhibits insulin action in the liver by interfering with the insulin signaling pathway and is considered an important molecular link between obesity and insulin resistance. Cinnamon, due to its polyphenols, has beneficial insulin-like effects in controlling blood sugar and lipids and can regulate appetite. Does six weeks of aerobic exercise and cinnamon extract affect the expression of the resistin gene in the liver tissue of male rats fed a high-fat diet? In the present experimental study, 50 rats were randomly divided into five equal groups (control, high-fat diet, cinnamon extract and high-fat diet, aerobic exercise and high-fat diet, aerobic exercise and cinnamon extract and high-fat diet). High-fat food emulsion was given daily by gavage at a dose of 1.5 mg/kg of body weight for six weeks. Cinnamon extract was given at a dose of 200 mg/kg of body weight by gavage. The exercise group participated in an aerobic exercise program on a treadmill for five days a week for six weeks. The treadmill incline was 15% throughout the exercise period. The treadmill speed also started from 25 m/min in the first week and increased to 30 m/min in the sixth week. The exercise duration started from 10 minutes per day in the first week and increased to 50 minutes per day in the sixth week. Real Time-PCR was used to examine the expression levels of genes or mRNA of the target proteins. The mean of the research variables between the groups were compared using one-way ANOVA and Bonferroni tests to determine between-group differences. The mean of the variables between each group and the control group were compared using one-sample t-test to determine within-group differences. Along with a similar pattern of significant increases, the difference in body weight and liver tissue weight at post-test was significant among all groups. The difference in the means of the resistin gene variable in the liver tissue of male rats fed a high-fat diet after six weeks of aerobic exercise, cinnamon extract consumption, and high-fat food was significant among all groups. Comparison of different groups with the control group also showed the effect of the high-fat diet, aerobic exercise, and cinnamon variables and their interaction on the changes in the resistin gene variable in liver tissue. High-fat food caused undesirable changes in the expression of this gene, but six weeks of exercise and cinnamon extract consumption moderated the undesirable effects of the high-fat diet and produced favorable changes in the expression of this gene, body weight, and liver tissue weight. In addition, the combination of six weeks of exercise and cinnamon extract had much more favorable effects in moderating the undesirable effects of the diet on the expression of this gene.
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