The Effects of acute consumption of BCAA,s on inflammatory and muscle damage indicators following intense resistance training session in girls professional athletes
Subject Areas : Sports Medicine and Treatment of Sports Injuries
Marjaneh Mehrabi
1
,
Bahram Abedi
2
,
Mojtaba Khansooz
3
1 - Master's degree student, Department of Physical Education, Islamic Azad University, Mahalat Branch, Mahalat, Iran
2 - Professor, Department of Sports Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran.
3 - Ph.D., Department of Sports Sciences, Arak University, Arak, Iran.
Keywords: Branched-Chain Amino Acid, Inflammatory Indexes, Muscle Damage Indexes, Intense Resistance Training,
Abstract :
The purpose of the present study was to determine the effect of acute BCAA supplementation on some inflammatory indicators and muscle damage following an intense resistance training session in female athletes. In this semi-experimental research with a pre-test-post-test design, 20 female athletes were randomly divided into 2 groups of 10: resistance training group with BCAA supplementation and resistance training group with placebo supplementation, one in the morning and one in the evening. A bout of resistance activity including 10 sets of eight repetitions of squats with 70% of 1RM using the Smith machine and a 3-minute rest between each set was performed by all participants. After completing the squat protocol, participants performed five consecutive sets of 20 repetitions (10 repetitions of split body weight jumps) with two minutes of rest between each set. The obtained data were analyzed using the dependent t-test and the covariance test with Benferroni's post hoc test using spss version 22 software. The results of the study showed that the serum levels of creatine kinase and the amount of delayed muscle pain and stiffness immediately, 24 and 48 hours and the serum levels of interleukin 6 immediately after intense resistance activity increased significantly compared to the baseline state. Also, the plasma levels of creatine kinase and the level of delayed muscle pain and stiffness in 24 hours and the level of interleukin-6 plasma levels have reached their peak after intense resistance activity, and this level was significantly higher than 48 and 72 hours after intense resistance activity. The present study showed that the consumption of BCAA supplement (0.22 g/kg) before and after exercise has no beneficial effect in reducing injury, muscle pain and inflammation in resistance trained women after senteric resistance training.
[1] Markus I, Constantini K, Hoffman JR, Bartolomei S, Gepner Y. Exercise-induced muscle damage: Mechanism, assessment and nutritional factors to accelerate recovery. Eur J Appl Physiol. 2021; 121:969–92.
[2] Owens DJ, Twist C, Cobley JN, Howatson G, Close GL. Exercise-induced muscle damage: What is it, what causes it and what are the nutritional solutions? Eur J Sport Sci. 2019;19(1):71–85.
[3] Howatson G, Van Someren KA. The prevention and treatment of exercise-induced muscle damage. Sport Med. 2008; 38:483–503.
[4] Tidball JG. Regulation of muscle growth and regeneration by the immune system. Nat Rev Immunol. 2017;17(3):165–78.
[5] Paulsen G, Ramer Mikkelsen U, Raastad T, Peake JM. Leucocytes, cytokines and satellite cells: what role do they play in muscle damage and regeneration following eccentric exercise? Exerc Immunol Rev. 2012;18.
[6] Koch AJ, Pereira R, Machado M. The creatine kinase response to resistance exercise. J Musculoskelet Neuronal Interact. 2014;14(1):68–77.
[7] Yu J-G, Fürst DO, Thornell L-E. The mode of myofibril remodelling in human skeletal muscle affected by DOMS induced by eccentric contractions. Histochem Cell Biol. 2003; 119:383–93.
[8] Gorissen SHM, Phillips SM. Branched-chain amino acids (leucine, isoleucine, and valine) and skeletal muscle. In: Nutrition and skeletal muscle. Elsevier; 2019. p. 283–98.
[9] Millward DJ, Layman DK, Tomé D, Schaafsma G. Protein quality assessment: impact of expanding understanding of protein and amino acid needs for optimal health. Am J Clin Nutr. 2008;87(5):1576S-1581S.
[10] Nicastro H, Da Luz CR, Chaves DFS, Bechara LRG, Voltarelli VA, Rogero MM, et al. Does branched-chain amino acids supplementation modulate skeletal muscle remodeling through inflammation modulation? Possible mechanisms of action. J Nutr Metab. 2012;2012.
[11] Jäger R, Kerksick CM, Campbell BI, Cribb PJ, Wells SD, Skwiat TM, et al. International society of sports nutrition position stand: protein and exercise. J Int Soc Sports Nutr. 2017;14(1):20.
[12] Tom A, Nair KS. Assessment of branched-chain amino acid status and potential for biomarkers. J Nutr. 2006;136(1):324S-330S.
[13] Rahimi MH, Shab-Bidar S, Mollahosseini M, Djafarian K. Branched-chain amino acid supplementation and exercise-induced muscle damage in exercise recovery: A meta-analysis of randomized clinical trials. Nutrition. 2017; 42:30–6.
[14] Hormoznejad R, Zare Javid A, Mansoori A. Effect of BCAA supplementation on central fatigue, energy metabolism substrate and muscle damage to the exercise: a systematic review with meta-analysis. Sport Sci Health. 2019; 15:265–79.
[15] da Luz CR, Nicastro H, Zanchi NE, Chaves DFS, Lancha Jr AH. Potential therapeutic effects of branched-chain amino acids supplementation on resistance exercise-based muscle damage in humans. J Int Soc Sports Nutr. 2011;8(1):23.
[16] Bessa AL, Oliveira VN, Agostini GG, Oliveira RJS, Oliveira ACS, White GE, et al. Exercise intensity and recovery: biomarkers of injury, inflammation, and oxidative stress. J Strength Cond Res. 2016;30(2):311–9.
[17] Pournot H, Bieuzen F, Louis J, Fillard J-R, Barbiche E, Hausswirth C. Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise. PLoS One. 2011;6(7): e22748.
[18] VanDusseldorp TA, Escobar KA, Johnson KE, Stratton MT, Moriarty T, Cole N, et al. Effect of branched-chain amino acid supplementation on recovery following acute eccentric exercise. Nutrients. 2018;10(10):1389.
[19] Tartibian, B., Maleki, B. H., & Abbasi, A. The effects of ingestion of omega-3 fatty acids on perceived pain and external symptoms of delayed onset muscle soreness in untrained men. Clinical Journal of Sport Medicine, 2009;19(2), 1 1 5-1 1 9.
[20] Lin, W., Song, H., Shen, J., Wang, J., Yang, Y., Yang, Y., ... & Lin, R. Functional role of skeletal muscle-derived interleukin-6 and its effects on lipid metabolism. Frontiers in Physiology, 2023; 14.
[21] Barnes, J. N., Trombold, J. R., Dhindsa, M., Lin, H. F., & Tanaka, H. Arterial stiffening following eccentric exercise-induced muscle damage. Journal of applied physiology, 2010;109(4), 1102-1108.
[22] Khemtong, C., Kuo, C. H., Chen, C. Y., Jaime, S. J., & Condello, G. Does branched-chain amino acids (BCAAs) supplementation attenuate muscle damage markers and soreness after resistance exercise in trained males? A meta-analysis of randomized controlled trials. Nutrients, 2021; 13(6), 1880.
[23] Jackman, S. R., Witard, O. C., Jeukendrup, A. E., & Tipton, K. D. Branched-chain amino acid ingestion can ameliorate soreness from eccentric exercise. Medicine & Science in Sports & Exercise, 2010;42(5), 962-970.
[24] Zhenyukh, O., Civantos, E., Ruiz-Ortega, M., Sánchez, M. S., Vázquez, C., Peiró, C., ... & Mas, S. High concentration of branched-chain amino acids promotes oxidative stress, inflammation and migration of human peripheral blood mononuclear cells via mTORC1 activation. Free Radical Biology and Medicine, 2017;104, 165-177.
[25] Hosios, A. M., Hecht, V. C., Danai, L. V., Johnson, M. O., Rathmell, J. C., Steinhauser, M. L., ... & Vander Heiden, M. G. Amino acids rather than glucose account for the majority of cell mass in proliferating mammalian cells. Developmental cell, 2016;36(5), 540-549.
[26] Fouré, A., Nosaka, K., Gastaldi, M., Mattei, J. P., Boudinet, H., Guye, M., ... & Gondin, J. Effects of branched-chain amino acids supplementation on both plasma amino acids concentration and muscle energetics changes resulting from muscle damage: A randomized placebo controlled trial. Clinical Nutrition, 2016;35(1), 83-94.
[27] Grazioli, R., Lopez, P., Machado, C. L., Farinha, J. B., Fagundes, A. O., Voser, R., ... & Cadore, E. L. Moderate volume of sprint bouts does not induce muscle damage in well-trained athletes. Journal of Bodywork and Movement Therapies, 2020;24(1), 206-211.
[28] Khemtong, C., Tessitore, A., Jaime, S. J., Gobbi, G., Jensen, J., Yang, A. L., ... & Condello, G. Branched-chain amino acids supplementation does not accelerate recovery after a change of direction sprinting exercise protocol. Nutrients, 2022;14(20), 4331.
[29] Rahimlou M, Ahmadi AHR, Palimi E, Mahdipour M, Poodeh BM. Reduction of Muscle Injuries and Improved Postexercise Recovery by Branched-Chain Amino Acid Supplementation: A Systematic Review and Meta-Analysis. J Fasting Heal. 2020;8(1).
[30] Shenoy, S., Dhawan, M., & Sandhu, J. S. Effect of chronic supplementation of branched chain amino acids on exercise-induced muscle damage in trained athletes. Journal of Sports Science, 2017;5, 265-273.
[31] Amirsasan, R., Nikookheslat, S., Sari-Sarraf, V., Kaveh, B., & Letafatkar, A. The effects of two different dosages of BCAA supplementation on a serum indicators of muscle damage in wrestlers. International Journal of Wrestling Science, 2011; 1(2), 32-6.
[32] Fernandes, J. F., Lamb, K. L., & Twist, C. Exercise-induced muscle damage and recovery in young and middle-aged males with different resistance training experience. Sports, 2019;7(6), 132.
[33] Barzegari A. Effect of 450 Mg. kg− 1 branched-chain amino acid supplement on muscle serum damage indices. World Appl Sci J. 2011; 15:1082–5.
[34] Brancaccio P, Limongelli FM, Maffulli N. Monitoring of serum enzymes in sport. Br J Sports Med. 2006;40(2):96–7.
[35] Nosaka K. Effects of amino acid supplementation on muscle soreness and damage. Int J Sport Nutr Exerc Metab. 2006;16(6):620–35.
[36] Wilke, J., & Behringer, M. Is “delayed onset muscle soreness” a false friend? The potential implication of the fascial connective tissue in post-exercise discomfort. International journal of molecular sciences, 2021;22(17), 9482.
[37] Wilke, J., Hespanhol, L., & Behrens, M. Is it all about the fascia? A systematic review and meta-analysis of the prevalence of extramuscular connective tissue lesions in muscle strain injury. Orthopaedic Journal of Sports Medicine, 2019;7(12), 2325967119888500.
[38] Dorrell, H., & Gee, T. The acute effects different quantities of branched-chain amino acids have on recovery of muscle function. Sports Nutrition and Therapy, 2016;1(3), e115-e115.
[39] Malm, C. J. A. P. S. Exercise‐induced muscle damage and inflammation: fact or fiction? Acta Physiologica Scandinavica, 2001;171(3), 233-239.
[40] Proske, U., & Morgan, D. L. Muscle damage from eccentric exercise: mechanism, mechanical signs, adaptation and clinical applications. The Journal of physiology, 2001;537(2), 333-345.
[41] Raizel, R., & Tirapegui, J. Role of glutamine, as free or dipeptide form, on muscle recovery from resistance training: A review study. Nutrire, 2018;43(1), 28.
[42] Street, B., Byrne, C., & Eston, R. Glutamine supplementation in recovery from eccentric exercise attenuates strength loss and muscle soreness. Journal of Exer cise Science & Fitness, 2011;9(2), 116-122.