Investigation the effect of Pterocarya fraxinifolia extract in eliminating some urinary tractinfection bacteria in comparison with ciprofloxacin

Subject Areas : Ecophysiology & Phytochemistry of Medicinal and Aromatic Plants

Masoud Azadbakht ¹ (Sana Institute Of Higher Education, Sari, Iran)

Received: 2023-02-25 Accepted : 2023-06-18 Published : 2023-06-21

Keywords: E. coli, antimicrobial effect, P. aeruginosa, Ciprofloxacin, K. pneumoniae, Pterocarya fraxinifolia leaf extract,

Abstract :

Urinary tract infection (UTI) is one of the most common infections in human body. The main cause of this infection is gram negative bacteria such as Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. The treatment of UTI is antibiotic antibiotic therapy. Today, antibiotic resistance is considered as an important challenge in treating infections. Pterocarya fraxinifolia is widely distributed in northern area of Iran and used as drug plant as far. The aim of this study was evaluation of antimicrobial effects of Pterocarya fraxinifolia leaf extracts (methanolic) compared to Ciprofloxacin against E. coli, K. pneumoniae and P. aeruginosa. To evaluate the antimicrobial effects, the diameter of inhibitory zone was measured using disk diffusion and well agar diffusion methods. In addition, the minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) were determined by macro dilution method. Disk diffusion results shows that no inhibitory zone for extract against E. coli, K. pneumoniae and P. aeruginosa in 10, 20, 30, 40 and 50 µl but Ciprofloxacin had inhibitory zone (31.33, 32.67 and 33 mm respectively). Agar diffusion results shows that inhibitory zone in 80, 90, 100 and 110 µl for E. coli (10.66, 12, 13 and 13.83 mm) K. pneumoniae (10, 12.33, 13 and 14.33 mm) and P. aeruginosa (12, 13, 14 and 15 mm). MIC was 8.33, 6.25 and 6.25 mg/ml respectively for E. coli, Kebsiella spp. and Pseudomonas spp. MBC was 16.67, 12.5 and 12.5 mg/ml respectively for E. coli, K. pneumoniae and P. aeruginosa. Based on data, Pterocarya fraxinifolia leaf extracts had antimicrobial effect against E. coli, K. pneumoniae and P. aeruginosa but not strong as Ciprofloxacin.