Phytochemical investigation of the extract of different organs of Silene conoidea. L. using gas chromatography-mass spectrometer in Sabzevar
Samira Eghbali
1
(
Department of Pharmacognosy and traditional pharmacy, School of Pharmacy, Birjand University of Medical Sciences, Birjand, Iran
)
Mino Qalichi
2
(
1Department of Chemistry, Faculty of Science, Gonbad Kavous University, Gonbad, Iran
)
Akram Taleghani
3
(
Department of Chemistry, Facualty of Science, Gonbad Kavous University, Gonbad, Iran
)
Keywords: Bioactive phytochemicals, Chloroform fraction, Dichloromethane fraction, Silene weed,
Abstract :
Silene weed plant (Silene conoidea. L) is a medicinal and edible plant from the Caryophyllacea family. The medicinal properties of this species are mostly attributed to its phenolic chemical compounds. This plant in traditional and modern medicine, used for treatment of skin infections, and blood coagulation which its medicinal properties are attributed to phenolic compounds. In this research, flower, stem, leaf and root of S. conoidea collected from Sabzevar in Spring 2022, the chemical compositions of chloroform and dichloromethane fractions were reported by GC-MS. In order to preparation of fractions for all the organs under the same conditions, petroleum ether was used for defatting, and chloroform and dichloromethane were used to extract the effective phytochemicals by maceration method. Analysis GC-MS of chloroform fractions showed that terpene derivatives, phenolic derivatives, hydrocarbons, fatty acids, heterocyclic derivatives, phenylpropanes, silane derivatives and steroids are the main constituent compounds in all organs. Hydrocarbon derivatives (69.16%) in flower, terpene derivatives (40.46%) in stem and fatty acid derivatives (40.46% and 63.98%) respectively in leaf and root are the most constituent compounds in chloroform fractions. Also, the analysis GC-MS of dichloromethane fractions showed that terpene derivatives (47.62% and 40.53%) in leaf and stem and silane derivatives (52.70%) in root are the most constituent compounds, respectively. The properties of solvents play an important role in determining the extraction of phytochemical compounds with unique biological properties. In general, the results of this research show that the non-polar extract of chloroform and the stem of plant have valuable effective substances, especially terpene compounds for medicinal purposes. These results suggested that other genetic resources of S. conoidea can be further explored to screen high bioactive compounds and purification of phytochemical compounds, which are valuable to produce, expand, and develop natural antioxidants in production of bio-medicine and food.