Phytochemical investigation and inhibitory effect of Thymus eriocalyx (Ronniger) Jalas essential oil on the growth of bacteria (Enterococcus faecalis and Salmonella typhimurium)

Subject Areas : Medicinal Plants

Somayeh Farahmand ¹ , Farshid Rezaei ² , Sahar kavosi ³

1 - Assistant Professor, Department of Biology, Payame Noor University (PNU), P.O.Box 19395-4697, Tehran, Iran
2 - - M.Sc, Department of Biology, Payame Noor University (PNU), P.O.Box 19395-4697, Tehran, Iran
3 - M.Sc. Department of Biology, Borujerd Azad University, Lorestan, Iran

Received: 2022-07-15 Accepted : 2022-09-20 Published : 2023-09-23

Keywords: Carvacrol, Salmonella typhimurium, Antibacterial, Enterococcus faecalis, Thymus eriocalyx (Ronninger),

Abstract :

Nowadays, due to the complications caused by the use of chemical drugs and the increase of antibiotic-resistant microbial strains, studies have been conducted to find antibacterial drugs from plant compounds. In this research, the aerial parts of Thymus eriocalyx were collected from the heights of Kermanshah in June 1400 (810 meters) in the flowering stage. The essential oil was extracted by distillation with water using a Cloninger machine. The antimicrobial activity of the essential oil of this plant in successive concentrations of 250, 125, 62.5, 31.25, 15.62, 7.81, 3.9, 1.95, 0.97 and 0.48 mg/ml against two bacterial species Enterococcus faecalis and Salmonella typhimurium by determining the minimum inhibitory concentration (MIC) in broth medium, minimum lethal concentration (MBC) in agar medium and disk diffusion were investigated. Ampicillin with a concentration of 10 mg/ml, ciprofloxacin 5 mg/ml, erythromycin 15 mg/ml and cefixime 5 mg/ml were used as positive controls. Mass gas chromatography method showed that this type of thyme has 42 effective substances. The most phenolic composition of the pasture ovia is carvacrol (66.96%). MBC for Enterococcus faecalis and Salmonella typhimurium bacteria was 15.67 and 62.5 mg/ml, respectively. 250 and 125 mg/mol discs were better than selected antibiotics in inhibiting both bacteria. The essential oil of pasture thyme plant contains antiseptic substances with antibacterial effects. The effect of essential oil in stopping the growth of Gram-positive bacteria was greater than that of Gram-negative bacteria. Considering that the essential oil of the plant had significant antibacterial effects compared to antibiotics in laboratory conditions, it is suggested to carry out more experiments to evaluate its effect in animal models.

References:

Akhavan, F., Tahmuzi Didehban, S. and Hojjati, M. 2020. Antibacterial effects of thyme, savory, rosemary, mint and peppermint essential oils on five gram-positive bacteria in comparison with effects of three antibiotics on the bacteria. Iranian Journal of Nutrition Sciences & Food Technology, 15(3): 97-103.

Alghamdi, F. and Shakir, M. 2020. The influence of Enterococcus faecalis as a dental root canal pathogen on endodontic treatment: A systematic review. Cureus, 12(3): e7257- 7267.

Ansari, N., Yazdian-Robati, R., Shahdordizadeh, M., Wang, Z. and Ghazvini, K. 2017. Aptasensors for quantitative detection of Salmonella Typhimurium. Analytical biochemistry, 533 (1): 18-25.

Batani, A., Yousefi Jovan, I., Far Hangi, M. and Zare Moghadam, M. 2015. Introduction of thyme medicinal plant, its planting, growing and harvesting The first national conference of medicinal, aromatic and spice plants, 1-12 https://civilica.com/doc/958923.

Davarpanah, M., Bakhtiari, R., Karimi, M., Hosseini, S.F. and Esmaeili, A. 2022. Iranian native medicinal plants affecting Staphylococcus aureus as septic pathogens: an updated review. Egyptian Journal of Veterinary Sciences, 53(1): 1-8.

Falahi, J., Ebadi, M., Rezvani, M. P., Hedayai, M. and Tarighi, S. 2010. Evaluation of essential oil of six mediclinal plant on controlling of Salmonella bacteria in comparison with streptomycin. Scientific-Research Iranian Veterinary Journal, 6(1): 25-33.

Farahmand, S., Rasooli, A. and Saffarpour, M. 2016. Antifungal activities of methanolic extract of plants. Electronic Journal of Biology, S, (1): 42-44.

Hasanloo, T., Sepehrifar, R. and Farahmand, S. 2015. The effect of different doses of silymarin, extracted from the seeds of the Silybum marianum L. Gaertn on the growth of 6 bacterial species. Research in Medicine, 38(4): 193-199.

Kaeidi, A., Rahmani, M.R. and Hassanshahi, J. 2020. The protective effect of carvacrol and thymol as main polyphenolic compounds of thyme on some biologic systems in disease condition: A narrative review. Journal of Rafsanjan University of Medical Sciences, 19(1): 81-96.

Matera, G., Altuvia, Y., Gerovac, M., El Mouali, Y., Margalit, H. and Vogel, J. 2022. Global RNA interactome of Salmonella discovers a 5′ UTR sponge for the MicF small RNA that connects membrane permeability to transport capacity. Molecular cell, 82(3): 629-644.

Mehran, M., Hosseini, H., Hatami, A. R., Taghizade, M. and Safaei, A. R. 2016. Investigation of seven species of essential oils of thyme and comparison their antioxidant properties. Journal of Medicinal Plants, 15(58): 134-140.

Miller, W.R., Murray, B.E., Rice, L.B. and Arias, C.A. 2020. Resistance in vancomycin-resistant enterococci. Infectious Disease Clinics, 34(4): 751-771.

Mohseni, N.M., Daraei Garmakhany, A. and Mohamadi Sani, A. 2018. Study of the effect of thyme essential oil on the reduction of peroxidase enzyme activity in the black Spanish radish and green bean. Journal of Food Science and Technology, 82(15): 63-71.

Salehi, M., Hasanloo, T., Mehrabian, S. and Farahmand, S. 2010. Effects of Silybum marianum (L.) Gaertn seeds extract on dermatophytes and saprophytes fungi in vitro compare to clotrimazol. Pharmaceutical Sciences, 16(4): 203-210.

Yousefzadeh, K., Houshmand, S., Shiran, B., Mousavi-Fard, S., Zeinali, H., Nikoloudakis, N., Gheisari, M.M. and Fanourakis, D. 2022. Joint effects of developmental stage and water deficit on essential oil traits (content, yield, composition) and related gene expression: A case study in two thymus species. Agronomy, 12(5): 1008-1027.