Phytochemical, antimicrobial and cytotoxicity effects of Rosa persica L. extracts in North Khorasan province

Subject Areas : Medicinal Plants

Ali Koohestanian ¹ , Maryam Taghizadeh ² , Maliheh Samadi Kazemi ³ , Ahmad Asgharzadeh ⁴ , Seyedeh Faezeh Taghizadeh ⁵

1 - PhD student, Department of Horticultural Sciences, Faculty of Agriculture, Shirvan branch, Islamic Azad University, Shiravan, Iran
2 - 2Assistant Professor, Department of Agriculture, Faculty of Agriculture, Shirvan branch, Islamic Azad University, Shiravan, Iran
3 - Assistant Professor, Department of Chemistry, Faculty of Science, Bojnord Branch, Islamic Azad University, Bojnord, Iran
4 - Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture, Shirvan branch, Islamic Azad University, Shiravan, Iran
5 - Postdoctoral, Pharmaceutical Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Iran

Received: 2022-02-13 Accepted : 2022-06-11 Published : 2022-11-27

Keywords: MIC, MBC, Cytotoxicity, Antibacterial, Rosa persica L,

Abstract :

The aim of this study was to Phytochemical, antimicrobial and cytotoxicity effects of Rosa persica L. extracts in a complete randomized design with three replications. R. persica L. was collected from Shirvan city located in North Khorasan province in the late summer of 2019 . The methanol, petroleum ether, ethyl acetate and water extracts were prepared by maceration method and were analyzed by using the liquid chromatography-mass spectrometer system, it was determined that the methanolic extract had the highest amounts of rosmarinic acid compounds. Antimicrobial effects of different extracts were investigated by determining the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). Antimicrobial assays against Staphylococcus aureus, S. epidermidis, Micrococcus luteus, Bacillus cereus, Pseudomonas aeruginosa, Salmonella typhi, Serratia marcescens, Escherichia coli, Candida albicans, Aspergillus niger showed that in all treatments, gram-negative bacteria were more resistance in compare to gram positive bacteria. In all extracts, the most sensitive strain was S. aureus with MIC and MBC of 10.89±0.13 and 19.34±0.33 μg/ml in the methanolic extract, respectively. Methanolic extracts of aerial parts had significantly stronger antimicrobial effects, respectively. Based on the results of cytotoxicity of different extracts on MCF-7, DU-145, PC3, A2780, C26, U-87-MG, MDA-MB-231, and Hela, it was found that Methanolic extract had the most cytotoxic effects on all cell lines studied. The lowest effects of cytotoxicity were related to the aqueous extract of aerial parts. In all treatments and cell lines, the IC50 was less than 400 μg/ml. The biochemical and biological activities of R. persia may be related to its phenolic compounds, so further studies is recommended.

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