Comparative evaluation of phenolic and Flavonoids compounds, antioxidant and antibacterial activity of methanol extract of Artemisia annua L, Thymus vulgaris L, Matricaria chamomilla L, Salvia officinalis L. and Pistacia atlantica var mutica

Subject Areas : Medicinal Plants

Auob mazaraie ¹ , leila fahmideh ²

1 - Ph. D. student of Biotechnology, University of Zabol, Iran
2 - Assistant Professor of Department of plant Breeding and Biotechnology, University of Zabol, Iran

Received: 2017-09-08 Accepted : 2018-10-29 Published : 2018-11-22

Keywords: Antioxidant, Phenolic, Antibacterial, Total flavonoid,

Abstract :

This study was investigated the relationship between the number of phenols, flavonoids, antioxidant, and antimicrobial properties of extracts of some medicinal plants. Aerial parts of plants in blooming of Artemisia annua (from Saravan City), Thymus vulgaris (from the Medicinal plants farm of Zabol University), Matricaria chamomill (from Farashband City), Salvia officinalis (from the Medicinal plants farm of Zabol University) and Fruit of Pistacia atlantica (from Saravan City) were collected from farms and habitats of Sistan and Baloochestan and Fars province in 2016. The plant extracts were obtained by maceration method, phenolic and flavonoid contents were determined by Folin-Ciocaltiu and Aluminum Chloride colorimetric, antioxidant activities were studied by method DPPH (2,2-diphenyl-1-picrylhydrazyl) and then antimicrobial activities were estimated by Disk-Diffusion method against two bacteria Staphylococcus aureus and Escherichia coli. The plant extract of Artemisia annua and Thymus vulgaris respectively had maximum total phenolic (22.36 mg/g DW) and flavonoid contents(142.59 mg/g DW) and antioxidant activity, (72.01 µg/ml and 70.75 µg/ml) and antibacterial activity against Staphylococcus aureus and Escherichia coli respectively with inhibition zone (15.1 and 16.8 mm ) was the most sensitive bacteria to plant extracts. Generally, the methanolic extract of the plants demonstrated different levels of antioxidant activity; in addition, the best antioxidant activity was related to Artemisia. Hence, it can be argued that these extracts of herb, instead of chemical drugs, can be used to treat infections. Furthermore, before using them all their side effects should be carefully investigated in in vitro accompanied by in vivo studies.