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  • Evaluation of total phenol and flavonoid content, antioxidant activity, anti-pain and anti-inflammatory of Ocimum basillicum var. purpurescens in comparison with opioid drug (morphine) in male rats

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Manuscript ID : EJMP-1702-1201 (R1) Visit : 194 Page: 91 - 104

20.1001.1.23223235.1396.5.1.8.7

Article Type: Original Research

Evaluation of total phenol and flavonoid content, antioxidant activity, anti-pain and anti-inflammatory of Ocimum basillicum var. purpurescens in comparison with opioid drug (morphine) in male rats

Subject Areas : Medicinal Plants

Solmaz Fakhari 1 , Isa bilejani 2 , Arash Khaki 3 , Asghar Rajabzadeh 4 , Masoumeh Mazandarani 5

1 - Department of Anesthesiology, Imam Reza Hospital, Tabriz University of Medical Sciences, , Iran
2 - 3Department of Anesthesiology, Shahid Madani Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.
3 - Department of Pathobiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
4 - 2Department of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran
5 - 5Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran

Received: 2017-02-20 Accepted : 2017-04-30 Published : 2017-05-22

Keywords: Phenol, Flavonoid, Antioxidant, Pain, Ocimum basilicum, SOD activity, Opioid drugs (morphine),

Abstract :

This study was carried out to evaluate the total flavonoide, phenols, antioxidant, anti-pain and anti-inflammatory in different extracts of Ocimum ebasillicum var. purpurescens in comparison with opioid drug (morphine) in male rats. The male Wistar rats with 250±10 g weighing was used and randomly divided to five groups. Group I (treated by 0.1mg/kg acetic acid, II-IV (treated by 0.7, 0.9 and 1 mg/kgof plantextract) , V (treated by 0.1 mg/kg morphine) and evaluated by Writhing test. The total phenol and flavonoids content was evaluated by follin-ciocalteus reagent, Ammonium chloride, the antioxidant activity was measured by DPPH method  and  serum levels of PGE2 and SOD activity measured by ELISA Kits.  According to results, the ethanol extract of plant had more content of secondary metabolites with total phenol (288.3±51.5 mg EGA/g) content, flavonoide content (154.7±1.04mg EGU/g) and the highest ability of free radicals scavenging (79.4±4.1%). There was a significant correlation between phenol- flavonoid ingredients to antioxidant activity rate and free radicals scavenging of plant (P<0.05, r<0.9) extract. High dosages of plant extract could be decreased chronic pains, in addition, serum level of PGE2 has been showed a significantly decreasing in groups treated by Ocimum basilicum extract and morphine in compared to untreated group. The serum SOD of treated groups (plant extract) was observed near to morphine group (P<0.05). Anti-inflammatory and anti-pain effects of this plant statically were observed next to morphine. It is found that steadily consumption of herbal usage, as antioxidant and anti-inflammatory, could be medicated to inflammatory treatment and long-term pains instead of opioid drugs.

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