Ethnopharmacology, total phenol, flavonoid and antibacterial effect of Allium cepa L. var. Ilkhichi in East Azarbaygan province

Subject Areas : Bacteriology

Yones Anzabi ¹

1 - 1Department of Pathobiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Received: 2015-12-26 Accepted : 2016-06-21 Published : 2017-02-19

Keywords: Phenol, Flavonoid, antibacterial, Allium cepa L. var. Ilkhichi, Azarbaygan, Ethno pharmacology,

Abstract :

Allium cepa L.var.Ilkhichi is an edible wild Onion in East of Azerbaygan province, which has been used as a tonic, nutritive and anti-infection. This study was evaluated by phytochemical, ethnopharmacology and antibacterial activity of Allium cepa L.var. Ilkhichi. ethanolic extract was obtained by maceration method, Total phenol (based on Gallic acid standard) and flavonoid (based on Quercetin) contents were obtained by spectrophotometry (Folin ciocalto and almonium chloride, respectively). Anti-bacterial activity of plant extracts were obtained by disc diffusion method against the 8 standard strains of food infectious bacteria and determinated by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) by micro dilution methods. The results were showed that ethanol extract of plant had good amounts of total phenol (351.52±2.12 mgGAEg-1) and total flavonoid contents (111.5±12.5 mgQUEg-1),respectively.  Antibacterial results were showed that the Enterococcus faecalis with MIC=62.5 µg/mL and MBC=125µg/mL was the best sensitive bacteria against plant extract, but in comparison between anti biogram tests results and Vancomycin did not show significant difference (p<0.05). According to the results, despite the fact that alcoholic extracts of Red Onions )Allium cepa L. var. Ilkhichi) apparently can be used as an antibacterial drug against the number of bacteria were important in terms of food  infection ,but it can not  replaced as an ant infection drug as a natural compound replacing of the common antibiotics, certainly. So it’s necessary in this subject for further evaluation in animal models and clinical cases.  

References:

1. Ahmad, I. and Aqil, F. 2007. In vitro efficacy of bioactive extracts of 15 medicinal plants against ESbetaL-producing multidrug-resistant enteric bacteria. Microbiological research, 162(3): 264-275.


2. Anzabi, Y., and Khaki, A. 2015. Antibacterial effects of the essential oils and ethanol extracts of the native plants; Ziziphora Clinopodioides on 3 species of urinary tract isolated bacteria in rats’ experimental model. Medical Journal of Tabriz University of Medical Sciences and Health Services, 37(3): 18-25.


3. Appendini, P. and Hotchkiss, J.H. 2002. Review of antimicrobial food packaging. Innovative .Food Science and Emerging Technologies, 3: 113-126.


4. Babayi, H., Kolo, I., Okogun, J.I., and Ijah, U.J.J. 2004. The antimicrobial activities of methanolic extracts of Eucalyptus camaldulensis and Terminalia catappa against some pathogenic microorganisms. Biokemistri, 16 (2): 106- 111.


5. Bouamama, H., Noel, T., Villard, J., Benharref, A., and Jana, M. 2006. Antimicrobial activities of the leaf extracts of two Moroccan cistus L. species. Journal of Ethnopharmacology,104(1-2): 104-107.


6. Burt, S. 2004. Essential oils: their antibacterial properties and potential application in food - a review. International Journal of Food Microbiology, 94(3): 223-53.


7. Canillac, N., and Mourey, A. 2001. Antimicrobial activity of the essential oil of picea excels on Listeria, Staphylococcus and coliform bacteria. Food Microbiology, 18: 261-268.


8. Chen, J.C., Huang, L.J., Wu, S.L., Kuo, S.C., Ho, T.Y., and Hsiang, C.Y. 2007. Ginger and its bioactive component inhibit enterotoxigenic Escherichia coli heat-labile enterotoxin-induced diarrhea in mice. Journal of agricultural and food chemistry, 55(21): 8390-7.


9. Jalali, M., Abedi, D., Ghasemi dehkordi, N., and Chaharmahali, A. 2006. Evaluation of antibacterial activity of ethanol extracts of some medicinal plants against Listeria monocytogenes. Journal of Shahrekord University of Medical Sciences, 8(3): 25-33.


10. Chitsaz, M. 2006. In vitro Evaluation of Antibacterial Effect of Stachys schtschegleevii. Daneshvar Medical Journal, 67:12-19.(In Persian)


11. Clinical and Laboratory Standards institute. 2013. Performance standards for antimicrobial susceptibility testing Twenty-Third Informational Supplement, M100-S23. CLSI. Pennsylvania.


12. Dubey, A., Mishra, N. and Singh, N. 2010. Antimicrobial activity of some selected vegetables. International Journal of applied biology and pharmaceutical technology, 3(1)994-999.


13. Ekwenye, U.N., and Elegalam, N.N. 2005. Antibacterial activity of ginger (Zingiber officinale roscoe) and garlic (Allium sativum L.) extracts on Escherichia coli and Salmonella typhi. International Journal of Molecular Medicine and Advance Sciences,1(4): 411- 416.


14. Embuscado, M., and Huber, K.C. 2009. Edible Films and Coatings for Food Applications.NY: Springer, New York, 416p.


15. Erfani, F., Hasandokht, M.R., Barzegar, M., and Jabari, A. 2006. Determination and comparison of some nutrients in seven Iranian spinach cultivars. Iranian Journal of Food Science, 3(2): 27-33. (In Persian)


16. Evajelene, V., and Nataragan, D. 2011. Evaluation of free radical scavenging activity and biological properties of Spinacia oleracea L. International Journal of engineering science and technology, 3(1): 25-30.


17. Fazlara, A., Sadeghi, E., and Rostami Soleimani, P. 2012. Study on the antibacterial effects of Cuminum cyminum essential oil on Listeria monocytogenes in Iranian white cheese. Iranian Journal of Food Science and Technology,9(35): 35-44.


18. Griffiths, G., Trueman, L., Crowther, T., Thomas, B. and Smith, B. 2002. Onions – A global benefit to health. Phytotherapy Research, 16: 603-615.


19. Karuppusamy, S., and Rajasekaran, K.M. 2009. High throughput antibacterial screening of plant extracts by Resazurin Redox with Special reference to medicinal plants of western ghats. Global Journal of Pharmacology, 3 (2): 63-68.


20. Mashak, Z., Moradi, B., Akhonzdade, A., Abasifar, A., and Gandomi, H. 2008. Study the behavior of Listeria monocytogenes during the production process of Iranian white cheese under the influence of Zataria multiflora Boiss essential oil. Journal of Medicinal Plants, 29:114-122.


21. Mazandarani, M., Momeji, A., and Zarghami Moghaddam, P. 2013. Evaluation of phytochemical and antioxidant activities from different parts of Nasturtium officinale R. Br. In Mazandaran. Iranian Journal of Plant Physiology, 3(2):659-664.


22. Mohan Nair, M.K., Vasudevan, P., and Venkitanarayanan, K. 2005. Antibacterial effect of black seed oil on Listeria monocytogenes. Journal of Food Control, 16(5):395-398.


23. Momeni, L., and Zamanzad, B. 2010. The antibacterial properties of Allium cepa (onion) and Zingiber officinale (ginger) extracts on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans isolated from vaginal specimens. Journal of Shahrekord University of Medical Sciences, 11(4):81-87.


24. Moreno, S., Scheyer, T., Romano, C.S., and Vojnov, A.A. 2006. Antioxidant and antimicrobial activities of Rosemary extracts linked to their polyphenol composition.Free Radical Research, 40(2): 223-231.


25. Nasirpour, M., Yavarmanesh, M. and Mohamadisani, A. 2014. Antibacterial effect of aqueous extract of Artemisia aucheri, Artemisia sieberi and Hyssopus officinalis L. on the food borne pathogenic bacteria. Journal of food sciences, 46(12):73-84. (In Persian)


26. Nelson, C.A. and Reginald, A.O. 2007. Antimicrobial properties of extracts of Allium cepa (Onions) and Zingiber officinale (Ginger) on Escherichia coli, Salmonella typhi and Bacillus subtilis. Internet Journal of Tropical Medicine, 3(2):1-7.


27. Nostro, A., Germano, M.P., Angelo, V.A., Marino, A., and Connatelli, M.A. 2000. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Letters in Applied Microbiology, 30(5): 389-394.


28. Parekh, J., and Chanda, S.V. 2008. Antibacterial activity of aqueous and alcoholic extracts of 34 Indian medicinal plants against some staphylococcus species. Turkish Journal of Biology, 32: 63-71.


29. Pourmorad, F., Hosseini Mehr, S.J. and Shahabimajd, N. 2006. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnology, 5(11): 1142-1145.


30. Razavi Rohani, S.M., Moradi, M., and Mehdizadeh, T. 2011. Antibacterial combined effects of nisin and onion essential oil under different concentration of NaCl and pH against Listeria monocytogenes in vitro. Food hygiene Journal, 1(3):25-34. (In Persian)


31. Singh, A., Singh, R.K., Bhunia, A.K. and Singh, N. 2003. Efficacy of plant essential oils as antimicrobial agents against Listeria monocytogenes in hotdogs. Journal of Food Science andTechnology, 36(8): 787-794.


32. Tajkarimi, M. M., Ibrahim, S.A. and Cliver, D.O. 2010. Antimicrobial herb and spice compounds in food. Journal of Food Control, 21:1199-1218.


33. Thuille, N., Fille, M., and Nagl, M. 2003. Bactericidal activity of herbal extracts. International Journal of Hygiene and Environmental Health, 206(3): 217-21.


34. Tiwari, B.K., Valdramidis, V.P., Donnell, C.P., Muthukumarappan, K., Bourke, P. and Cullen, P.J. 2009. Application of natural antimicrobial for food preservation. Journal of Agricultural Food Chemistry, 57: 5987-6000.