Survey of Phytochemical, antioxidant and optimization of extraction methods for the best method determination of curcumin extraction from ethanol extracts of Curcuma longa L.
Subject Areas :
Medicinal Plants
samaneh Noori
1
,
Alireza Kiasat
2
,
Maryam Kolahi
3
,
Roya Mirzahani
4
,
Sid Mansur Seyyed Nezhad
5
1 - 4Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 - 4Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 - 5Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
4 - 4Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
5 - 5Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Received: 2016-09-28
Accepted : 2016-11-16
Published : 2016-12-20
Keywords:
Total Phenol,
phytochemistry,
Natural Antioxidant,
Turmeric,
Abstract :
According to the World Health Organization (WHO), the medicinal plants are the best source to obtain a variety of drugs. Nowadays, thousands of plant metabolites are being successfully used for the treatment of a variety of diseases. Turmeric (Curcuma longa L.), which holds the unique characteristics of the health sector worldwide known as a functional food ingredient. In the present study, an effective ingredient in turmeric rhizome (curcumin) was extracted with ethanol as a solvent by three methods: maceration, ultra-sonic and Soxhlet; then, the yield of extracts from the three methods were calculated. The HPLC technique was used to determine the amount of derived curcumin. Furthermore, the amount of curcuminoids was obtained by thin layer of Chromatography (TLC). The total phenolic content was measured by Folin-ciocalteu reagent and the antioxidant properties was evaluated by DPPH method. The highest extraction yield was obtained from Soxhlettechnique. The results from HPLC were showed that the maximum amount of curcumin was obtained from maceration method. The thin Layer of Chromatography confirmed the presence of curcuminoids in three methods of extraction. The highest phenolic content was reported from maceration method. And the most antioxidant properties were obtained via maceration and ultrasonic extraction method. The conclusions present the importance of determination extraction method to isolate curcumin as a bioactive compound and its importance that is widely used in the treatment of many diseases as well as new drugs synthesized; moreover, the maceration extract is in priority in the maximum amount of curcuminoid, antioxidant and phenolic content.
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