Evaluation and Comparison of Morphological and Phytochemical Characteristics of 14 Ziziphus jujuba Mill's Ecotypes in Iran

Subject Areas : Phytochemistry

sakineh kohansal vajargah ¹ , reza baradaran ² , seyyed gholamreza mosavi ³ , majid tolyat abolhasani ⁴ , darab yazdani ⁵

1 - Faculty member of Research Group for Optimization and Processing of Medicinal Plants, ACECR, South Khorasan Branch, Birjand, Iran
2 - Faculty of Islamic Azad University, Birjand Branch
3 - Faculty of Islamic Azad University, Birjand Branch
4 - Faculty of ACECR
5 - Faculty of ACECR

Received: 2018-08-30 Accepted : 2019-06-15 Published : 2019-05-22

Keywords: Ecotype, Morphological, Phytochemical, Ziziphus jujuba Mill,

Abstract :

The Zizyphus jujuba Mill from Rhamnaceae family is a laxative, a blood purifier, and a nerves relaxant. The study was performed to evaluate the morphological traits and the phenolic, tannin, and flavonoid compounds of Ziziphus jujuba Mill's ecotypes to achieve superior ecotype. 14 Ziziphus jujuba Mill's ecotypes from collection of ACECR in Southern Khorasan which had been obtained from flower and leaves in flowering stage and from fruit in fructifying stage, from different regions, in spring 2017. The Total amount of tannin and phenolic compounds were determined by Makar method the total amount of flavonoid was obtained using a high performance liquid chromatography apparatus. Grouping the ecotypes was carried out based on morphological and phytochemical traits, through cluster analysis. And correlation of quantitative traits was performed by Pearson method. The results showed that flavonoids yield had a significant positive correlation with fruit's length and width and length/width ratio. Phenol yield had a significant positive correlation with fruit's length and width. And tannin yield showed a significant positive correlation with phenol. The results of variance analysis, morphological traits including leaf's length and width and length/width ratio, fruit's length and width and length/width ratio, and thousand-seed weight showed a variety of germplasms of Ziziphus jujuba Mill in Iran. In terms of morphological traits, the coarse ecotype had the maximum effective ingredient. showed a statistically significant difference in phytochemicals. Based on cluster analysis, 14 ecotypes were divided into two separate groups. The evaluation of morphological and phytochemical traits among ecotypes a high variation.

References:

 

References

 

1. Balta, M.F.,Celik, F., Turkoglu, N., Ozrenk, K. and Ozgokce, F., 2009.Some fruit traits of Hawthorn (Cratagus spp.) genetic resources from Malatya, Turkey. Research Journal of Agriculture and Biological Sciences. 2: 531-536.


2. Chang, Q., Zuo, Z., Harrison, F. and Chow, M.S., 2002. Hawthorn. The Journal of Clinical Pharmacology, 42: 605-612.


3. Chiti, S., Mortazavi, S.A., Basiri, Sh. and Sharifi, A., 2013. Evaluation of some physicochemical and nutritional characteristics of two jujube cultivars in Khorasan province. The first regional conference of medicinal herbs in the north of the country.

http://www.civilica.com /Paper-RCMPNI01-RCMPNI01_026.html.

1. Davis, F.S. and Albrigo, L.G., 1994. Citrus. CAB international press, Wallington, UK. P. 9814.


2. Hossein Ava, S. and Saifi. A., 2002. jujube. Technical Publications of the Tate Organization Promotion. Tehran: 17


3. Ghos, K., 2009. Forgotten Fruit Jujube, South Khorasan Jihad Organization, Saeedi Manesh Publications.


4. Khakdaman, H., Pourmidani, A. and adenani, M., 2006. Genetic variation of different ecotypes of jujube (Zizyphus jujuba Mill.) In Iran using cluster analysis. Genetic research and improvement of Iranian pasture and forest plants. 14: 214-202.


5. Khirakhosyan, A., Kaufman, P., Warber, S., Zick, S., Aaronson, K., Bolling, S. And Change, S.C., 2014. Applied environmental stresses to enhance the levels of polyphenolics in leaves of hawthorn plants. Physiologia Plantarum, 121: 182- 186.


6. Makkar, H.P., 2003. Effects and fate of tannins in ruminant animal adaptation to tannins and strategies to overcome detrimental effect of feeding tannin-rich feed. Small Ruminant Research. 49: 241-256.


7. Martin. M. and Meyer, J.R., 1971. Phosphours utilization from internal and external sources in the growth of woody ornamental plants. Amer. Soc. Hort. Sci 96 (2): 209-212.


8. Mirhaydr, H., 1993. Plant Education: The use of medicinal herbs in the prevention of disease treatment (Vol. 2), Office of the Publishing of Islamic Culture.


9. Mirza., M. and sefidkon, F., 1999. Essential oil composition of two Salvia species from Iran, Salvia nemorosa L. and Salvia reuterana Boiss. Flover and fragrance J. 14: 230-232.


10. Nakhaei, A., 2016. Atlas of genetic reserves of garden products in South Khorasan province. Fekre bekr Press, Tehran.


11. Obeso, J.R. and Herrera, C.M., 2004. Inter-and intraspecific variation in fruit traits in co-occurring vertebrate-dispersed plants. International Journal of Plant Science, 55: 382-387.


12. Omidbigi, R., 2009. Production and processing of medicinal plants. Astan Quds Razavi Publishing House, Vol. 4, Fifth Edition, 438pages.


13. Pouyan, M., 2016. Medicinal plants of Southern Khorasan, Islamic Azad University, Birjand.


14. Rastegar, S. and Hasanzadeh, H., 2015. Evaluation of Quantitative and Qualitative Characteristics of Fruit of 11 Indigenous (Ziziphus mauritiana) Genotypes in Hormozgan Province. Plant Products of Shahid Chamran University - Faculty of Agriculture. 38(3): 0254- 3648.


15. Salehi, F., Aroee, H., Naghdi badi, H., Nemati, S. and Tolyat, M., 2017. Evaluation of morphophysiological and phytochemical traits of different ecotypes (Salvia multicaulis Vahl.) of Hamedan province. Journal of Medicinal Plants. Sixteenth Year, Fourth Edition, Special Issue No. 11.


16. Westwood, M., 1992. Working fruit in temperate regions (Translation Y. Rasool zadegan). Isfahan University Press.


17. Zargari, A., 1992. Medicinal Plants. first volume. Tehran University Press.