Aut ecology and phytochemical survey of Ziziphora clinopodioides Lam. with ethnopharmacology and floristic spectrum of medicinal plants in Bovanlou region (Northern Khorasan province)

Subject Areas : Medicinal Plants

aazam ghaviandam bovanlo ¹ , masoumeh Mazandarani ²

1 - laboratory expert-golestan university
2 - 2Accociated Professor of Biology, Islamic Azad University, Gorgan Branch, Gorgan, Iran

Received: 2017-03-07 Accepted : 2017-08-26 Published : 2017-11-22

Keywords: Ethnopharmacology, Ziziphora clinopodioides Lam, Total phenol and flavonoid, Northern Khorasan Province,

Abstract :

This study was in order to investigation of morphological, ecological requirement, phenology, ethnopharmacological information and phytochemical of Ziziphora clinopodioides Lam. in northern Khorasan province (Bovanlou -1728 m), randomly. In many field observation, the important ecological , phonological parameters and the ethnopharmacological data were  recorded . The aerial parts of plant in blooming were collected in July, 2013 and dried.  The hydro alcoholic extract was obtained by maceration method. The amounts of total phenols and flavonoids were determined in three replicates by using the Folin–Ciocalteu and aluminum chloride colorimetric methods, respectively. The result were showed that the plant vegetative growth occurs in May, followed by flowering in the late June till the end of December. The seeds ripening is achieved during the late November. Based on a floristic study, 30 plant species from 29 genera were identified  belonging to 17 families. The richest families were Asteraceae with 6 species (20%) and Lamiaceae with 4 species (13%), respectively. The highest proportion of life forms belonged to therophytes (43%) and geophytes (27%), respectively. Likewise, the highest proportion of chorotypes belonged mediterranean elements (40%), followed by to Irano-Touranian elements (36%). The ethnopharmacological survey were showed that it has been used traditionally as antiseptic and stimulating to treat of gastric ailments, fever few, common cold and digestive discomfort. According to results, TF and TP content of plant were 21.2 ± 0.19 mg QUE g-1 DW and 91 ± 0.06 mg GAE g-1 DW respectively.  

References:

1. Alp, S., Ercisli, S., Dogan, H., Temim, E., Leto, A., Zia-Ul-Haq, M., Hadziabulic, A. and Aladag, H. 2016. Chemical composition and antioxidant activity Ziziphora clinopodioides ecotypes from Turkey. Romanian Biotechnological Letters, 21(2): 11298-11303.


2. Aprotosoaie, A.C., Rӑilemeanu, E., Trifan, A. and Cioancӑ, O. 2013. The polyphenolic content of common Lamiaceae species available as herbal tea products in Romanian pharmacies. Revista Medico-Chirurgicala a Societatii de Medici si Naturalisti din Iasi, 117(1): 233-237.


3. Bakhshi Khaniki, G., Sefidkon, F. and Dehghan, Z. 2010. The effects of some ecological factors on essential oil yield and composition of Ziziphora clinopodioides Lam. Journal of Herbal Drugs, 1(1): 9-11. (In Persian).


4. Beikmohammadi, M. 2011. The evaluation of medicinal properties of Ziziphora clinopodioides. World Applied Sciences Journal, 12(9): 1635-1638.


5. Chang, C.C., Yang, M.H., Wen, H.M. and Chern, J.Ch. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analaysis, 10(3): 178-182.


6. Chevallier, A. 1996. The encyclopedia of medicinal plants: a practical reference guide to over 550 key herbs and their medicinal uses. Darling Kindersley, London, New York, Sydney, Moscow, 336.


7. Davis, P.H. 1965-1988. Flora of Turkey. Vols. 1-10. University of Edinburgh Press, Edinburgh.


8. Eisenman, S.W., Zaurov, D.F. and Struwe, L. 2012. Medicinal plant of central Asia: Uzbekistan and Kyrgyzstan. Springer New York Heidelberg Dordrecht London, 351p.


9. Gulcin, I., Topal, F., Sarkaya, S.B.O., Buesal, E., Bilsel, G. and Goren, A.C. 2011. Polyphenol contents and antioxidant properties of medlar (Mespilus germanica L.). Records of Natural Products, 5(3): 158-175.


10. Gulluce, M., Orhan, F., Adiguzel, A., Bal, T., Guvenalp, Z. and Dermirezer, LO. 2013. Determination of antimutagenic properties of apigenin-7-O-rutinoside, a flavonoid isolated from Mentha longifolia (L.) Huds. ssp. Longifolia with yeast DEL assay. Toxicology and Industrial Health, 29: 534-40.


11. Gursoy, N., Sihoglu-tepe, A. and Tepe, B. 2009. Determination of in-vitro antioxidative and antimicrobial properties and total phenolic contents of Ziziphora clinopodioides, Cyclotrichium niveum and Mentha longifolia subsp. typhoides var. typhoides. Journal of Medicinal Food, 12: 684-689.


12. Hashemi, S.M., Eghdami, A. and Sadeghi, F. 2011. Antioxidant activity and high performance liquid chromatography analyzation of methanolic and aqueous extract of Stachys inflata. The Journal of Organic Chemistry, 2(1): 36-43.


13. Komarov, V.L. (ed.) 1934-1954. Flora of USSR. Vol. 1-21. Izdatel’stvo Akademi Nauk SSSR Leningrad (English translation from Russian, Jerusalem, 1968-1977).


14. Mazandarani M., Zarghami Moghaddam, P., Baiat, H., Zolfaghari, M., Ghaemi, E., and Hemati, H. 2011. Antioxidant activity, phenol, flavonoid and anthocyanin contents in various extracts of Onosma dichroanthum Boiss. in north of Iran. Iranian Journal of Plant Physiology, 1(3): 169-176.


15. Meda, A., Lamien, C.E., Romito, M., Millogo, J. and Nacoulma, O.G. 2005. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chemistry, 91: 571-577.


16. Mirzaei-Aghsaghali, A., Syadati, S.A. and Fathi, H. 2012. Some of thyme (Thymus vulgaris) properties in ruminant's nutrition. Annals of Biological Research, 3(2): 1191-1195.


17. Olajire, A.A. and Azeez, L. 2011. Total antioxidant activity, phenolic, flavonoid and ascorbic acid contents of Nigerian vegetables. African Journal of Food Science and Technology, 2(2): 22-29.


18. Pourmorad, F., Hosseini Mehr, S.J. and Shahabimajd, N. 2006. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnology, 5(11): 1142-1145.


19. Raunkiaer, C. 1934. The life forms of plants and statistical plant geography. Clarendon Press, Oxford.


20. Rechinger, K.H. (ed.) 1963-2010. Flora Iranica, No. 1-178. Graz: Akademische Druck-und Verlasanstalt (1-174), Wien: Naturhistorisches Museum (175-178).


21. Salehi, P., Sonboli, A., Eftekhar, F., Nejad Ebrahimi, S. and Yousefzadi, M. 2005. Essential oil composition, antibacterial and antioxidant activity of the oil and various extracts of Ziziphora clinopodioides subsp. Regida (Boiss) Rech. F. from Iran. Biological and Pharmaceutical Bulletin, 28(10): 1892-6.


22. Senejoux, F., Demougeot, C., Kerram, P., Aisa, H.A., Berthelot, A., Bevalot, F. and Girard-Thernier, C. 2012. Biossay-guided isolation of vasorelaxant compounds from Ziziphora clinopodioides Lam. (Lamiaceae). Fitoterapia, 83(2): 377-382.


23. Sherafati Chaleshtori, R., Rafieian Kopaei, M., Rokni, N., Mortezaei, S. and Sherafati Chaleshtori, A. 2013. Antioxidant activity of Zataria multiflora hydroalcoholic extract and its antibacterial effect on Staphylococcus aureus. Journal of Mazandaran Medical Sciences University, 22(1): 88-94. (In Persian).


24. Shi, Y., Bi, K., Xu, T.H. and Tian, S.G. 2009. The stability of the essential oil from Uygur medicine Ziziphora clinopodioides Lam. Chinese Journal of Ethnomedicine and Ethnopharmacy, 5: 1-3.


25. Šmejkal, K., Malaník, M., Zhaparkulova, K., Sakipova, Z., Ibragimova, L., Ibadullaeva, G. and Žemlička, M. 2016. Kazakh Ziziphora species as sources of bioactive substances. Molecules, 21: 1-54.


26. Sonboli, A., Salehi, P. and Nejad Ebrahimi, S. 2005. Essential oil composition and antibacterial activity of the leaves of Stachys schtschegleevii from Iran. Chemistry of Natural Compound, 41: 171-174.


27. Takhtajan, A. 1986. Floristic region of the world. University of California Press, Berkeley, Los Angeles, London.


28. Talebi, S.M., Rezakhanlou, A. and Salahi Isfahani, G. 2012. Trichomes Plasticity in Ziziphora tenuior L. (Labiatae) in Iran: An ecological review. Annals of Biological Research, 3(1): 668-672.


29. Tian, S.,  Yu, Q.,  Wang, D. and  Upur, H. 2012. Development of a rapid resolution liquid chromatography-diode array detector method for the determination of three compounds in Ziziphora clinopodioides Lam from different origins of Xinjiang. Pharmacognosy Magazine, 8 (32): 280-284.


30. Townsend, C.C., Guest, E., and Al-Ravi, A. 1966-1980. Flora of Iraq. Vol. 1-9. Ministry of Agriculture of the Republic of Iraq, Baqdad.


31. Zarezadeh, A., Rezaei, M.B., Mirhosseini, A. and Shamszadeh, M. 2007. Ecological investigation of some aromatic plants from lamiaceae family in Yazd province.  Iranian Journal of Medicinal and Aromatic Plants, 23(3): 432-442. (In Persian).


32. Zargari, A. 1990. Medicinal Plants. Tehran University Press, Tehran, Iran, 976p. (In Persian).


33. Zohary, M. 1973. Geobotanical foundations of the Middle East. 2 vols. Fischer Verlag, Stuttgart, Amsterdam.


34. http://www.ipni.org/