Phytochemical, antioxidant and antidiabetic activity in aqueous extract of Trigonella foenumgraceum L.
Subject Areas : Environmental ecologySeed Mehrdad Kassaee 1 , Mohammad Taghi Goodarzi 2 , Nashim Hayati Rudbari 3 , Parecher Yaghmaei 4
1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Research Center of Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Keywords: Phenol, Flavonoid, Antioxidant, Advanced glycation end products, Anti-diabetic, Trigonella foenumgraceum L,
Abstract :
Trigonella foenumgraceum L. with variety of polyphenoles which has been used as an antioxidant and nutritive vegetable to treat of diabetes. The aim of the present study was to investigate the phytochemical, antioxidant and antidiabetic activities of plant leave extract. The leaves were collected from medicinal garden of Hamedan during August 2014. The aqueous extract was obtained by maceration. Total phenol and flavonoid content were determined by using spectrophotometric methods. The antioxidant activity were measured by using FRAP, ferric chelating activity, thiol and DPPH radical scavenging assay. The anti-diuretic properties, inhibition of fructose amine and advanced glycation end products formation and also albumin fragmentation by spectrophotometric, spectrofluorometric and electrophoresis methods, respectively. The results were showed that the total phenol content (23.87mg GAE/g), total flavonoid (17.29 mgQUE/g), thiolgrou (p0.26 mmoleCYE/mg) and total antioxidant capacity (0.33mmole FeSO4E/mg). Ferric chelating ability and radical scavenging activities were both in a concentration-related manner. The aqueous extract of plant in high concentration could inhibit fructosam ineformation (70.9%) and also albumin fragmentation. Thus this extract can be used for prevention of plant -related complications of diabetes.
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