Effect of UV-B radiation, gamma radiation and essential oil of Satureja mutica Fisch & C. A. Mey on the activity of microbial pathogens

Subject Areas : Medicinal Plants

Fatima Mahdinavasi ¹ , Bohlool Abbas zadeh ^{2 *} , راضیه عظیمی ³ , Vahid Abdossi ⁴ , Marjan Dyanat ⁵

1 - Islamic Azad University, Science and Research Branch, Faculty of Agriculture and Food Science
2 -
3 - عضو هیات علمی موسسه جنگلها و مراتع کشور، تهران
4 - دانشیار، گروه علوم باغی و زراعی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
5 - agriculture and food science faculty, Science research branch, Islamic azad university

Received: 2024-09-13 Accepted : 2025-02-02 Published : 2025-03-21

Keywords: Disinfection Escherichia coli gamma rays mold Satureja mutica yeast,

Abstract :

This research was conducted to study the effect of UV-B rays and gamma rays on the disinfection of plants harvested from the field and also to study the antimicrobial activity of Satureja mutica essential oil in the form of 3 separate experiments. In the first experiment, harvested plants were treated with UV-B for 0, 2.5, 5, 7.5, and 10 hours. In the second experiment, samples were exposed to gamma radiation at 0, 2.5, 5, 7.5, and 10 kGy. In the third experiment, the antimicrobial effect of the essential oil of fresh S. mutica on Escherichia coli, Pseudomonas fluorescens and Staphylococcus aureus was investigated. The effect of UV-B radiation on Enterobacteriaceae, Staphylococcus aureus, and total molds and yeasts showed that the highest inhibition of microbial activity was achieved with UV-B rays for 10 hours. The antimicrobial effect of gamma rays on Enterobacteriaceae, Staphylococcus aureus, Salmonella and all molds and yeasts was significant at the 1% level. The microbial activity of Enterobacteriaceae, Staphylococcus aureus, Salmonella, and total molds and yeasts was determined to be 2.24%, 1.83%, 0.79%, and 10.8%, respectively, at 10 kGy gamma irradiation as the optimal condition. The effect of essential oil concentration on the percentage of microbial contamination showed that the highest inhibition of growth of the studied bacteria was observed in the pure essential oil treatment, so that the activity of Escherichia coli was reduced by 41.3%, Pseudomonas fluorescens by 16.7% and Staphylococcus aureus by 42.3%. The results showed that the use of UV-B radiation was not effective for disinfecting plants. The use of gamma radiation of 7.5 kGy and more was very successful in controlling microbial agents, and the use of Satureja mutica essential oil in controlling microbial agents had promising results.

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