Investigation of the Effect of Biofertilizers on Morphophysiological and Biochemical Traits in Garden Thyme (Thymus vulgaris L.) Under Salinity Stress

Subject Areas : Medicinal Plants

Sayedeh Zahra Mousavi Gheidari ¹ (Department of Genetic and plant Breeding, Faculty of Agriculture and Natural Resources. Imam Khomeini International University, Qazvin. Iran. )
Raheleh Khademian ² (Department of Genetic and plant Breeding, Faculty of Agriculture and Natural Resources. Imam Khomeini International University, Qazvin. Iran. )
Sudabeh Mafakheri ³ (Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources. Imam Khomeini International University, Qazvin. Iran)

Received: 2024-04-27 Accepted : 2024-09-16 Published : 2024-09-16

Keywords: Azotobacter, Medicinal Plant, Salinity Stress, Symbiotic Fungus, Thyme,

Abstract :

To examine the role of symbiotic microorganisms in mitigating or reducing the negative effects of salinity stress on garden thyme, a greenhouse experiment was conducted in a factorial design with three replications at the International Imam Khomeini University. In this study, the effects of coexistence of Trichoderma harzianum fungus, Rhizobium lantis bacteria, and a mixture of fungus and bacteria at three salinity levels (0, 50, and 100 mM) on morphophysiological and biochemical traits of garden thyme were evaluated. Morphological traits assessed included root length, stem length, and wet and dry weights of roots and stems. Additionally, physiological and biochemical traits such as malondialdehyde content, electrolyte leakage, enzymatic antioxidants such as catalase and peroxidase, non-enzymatic antioxidants such as proline, total phenol and flavonoid content, as well as DPPH free radical scavenging activity, were evaluated. The results showed that salinity stress significantly reduced root and stem length, wet and dry weights of roots and stems in garden thyme. Furthermore, oxidative stress induced by salinity increased the activity of antioxidant enzymes (catalase and peroxidase) and total phenol and flavonoid content. On the other hand, treatment with T. harzianum fungus and R. lantis bacteria alleviated the negative effects of increased salinity concentration by improving the measured traits, including increased activity of antioxidant enzymes, decreased lipid peroxidation (MDA), and reduction in secondary metabolites, thereby reducing cell damage and promoting plant growth. In most cases, the simultaneous application of fungus and bacteria had a greater effect on the studied traits compared to the individual use of these symbiotic microorganisms.

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