Colorimetric determination of glucose using Fe3O4/CeO2/C-dots magnetic nanocomposite
Subject Areas : Determination of SpeciesYasaman Ezhdehakosh Abolverdi 1 , Fatemeh Honarasa 2
1 - Department of Chemistry, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2 - Department of Chemistry, Shiraz Branch, Islamic Azad University, Shiraz, Iran
Keywords: Nanoceria, Nanozymes, Carbon dots, glucose, Magnetic nanoparticles,
Abstract :
In the present study, determination of glucose was performed based on peroxidase-like activity of Fe3O4/CeO2/C-dot nanocomposite. In this way, a cascade reaction was used. At first, glucose in the presence of glucose oxidase enzyme reacts with oxygen to produce gluconic acid and H2O2. Then, in the second step, H2O2 in the presence of Fe3O4/CeO2/C-dot converts TMB (3,3’,5,5’- tetramethylbenzidine) to oxTMB which causes the solution to turn blue. Intensity of blue color depends on glucose concentration, nanocomposite amount, TMB concentration and solution pH. Absorbance of solution at 650 nm was increased by increasing the glucose concentration. So, linear range between glucose concentration and intensity of absorbance at 650 nm was observed. Experimental detection limit for glucose detection was obtained as 1×10-5 M. Also, the sensing system shows high selectivity towards the detection of glucose in the presence of fructose, lactose and maltose as interferences.
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