Synthesis of ZnO:Ag nanoparticles and Evaluation its antimicrobial activity against common Isolated bacterial pathogens from dairy products

Subject Areas : Biotechnological Journal of Environmental Microbiology

Khosro Issazadeh ¹

1 - Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran

Received: 2023-07-15 Accepted : 2023-08-11 Published : 2023-08-21

Keywords: Nanoparticle, Zinc oxide, Staphylococcus, Escherichia coli, Antibiogram,

Abstract :

Synthetic nanoparticles have unique physical and chemical properties. The most important characteristic of these nanoparticles is having a higher surface area than their counterparts of larger size. In this research 45 samples of dairy raw products after dilution of samples, to isolate Staphylococcus aureus and Escherichia coli were transferred to the Baird Parker Agar and Sorbitol Mac Conkey Agar media respectively, and were identified using a series of specific tests. Zinc oxide-doped nanopowder was synthesized by the sol-gel method. The antimicrobial effects of nanoparticles were investigated by the well diffusion method. The minimum inhibitory concentration (MIC) of Zinc oxide-doped nanopowder and minimum bactericidal concentration (MBC) were determined. The mean diameter zone of the inhibitory growth of strains of E. coli PTCC 1399 and E. coli (1) and E. coli (2) were 22.5, 18.5, and 15.4 mm respectively at a concentration of 50 mg/ml and the mean diameter zone of the inhibitory of S. aureus PTCC 1189, S. aureus (1) and S. aureus (2) standard strains were 24.5, 20.4 and 19.5 mm. In this concentration. MIC for E. coli PTCC 1399 was 1.75, and E. coli (isolate 1) and E. coli (isolate 2) were 1.55 and 3.13 mg/ml, respectively. In the case of further experiments, this nanoparticle can be used as a preservative.

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