Preparation of Magnetite Nanocomposites based on Azolla Filiculoides fern with Environmental Applications for Organic Pollutants

Subject Areas : Biotechnological Journal of Environmental Microbiology

Reyhaneh Kouchakinejad ¹ , Shahab Shariati ² , Jafar Abolhasani ³ , Ebrahim Ghorbani-Kalhor ⁴ , Mohammad Taghi Vardini ⁵

1 - Department of Chemistry, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 - Department of Chemistry, Rasht Branch, Islamic Azad University, Rasht, Iran
3 - Department of Chemistry, Tabriz Branch, Islamic Azad University, Tabriz, Iran
4 - Department of Chemistry, Tabriz Branch, Islamic Azad University, Tabriz, Iran
5 - Department of Chemistry, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Received: 2023-06-10 Accepted : 2023-06-29 Published : 2022-07-01

Keywords: nanocomposite, Pesticide, Chlorpyrifos, Azolla filiculoides, Magnetic Solid phase Extraction,

Abstract :

Azolla Filiculoides fern was utilized as a natural adsorbent to develop a magnetic solid phase extraction method for the preconcentration and determination of trace amounts of chlorpyrifos pesticide, which is a primary environmental concern. In this study, the magnetic solid phase extraction (MSPE) method using magnetite-modified azolla nanocomposites (azolla@Fe3O4 NCs) was developed to preconcentrate trace amounts of chlorpyrifos pesticide prior to UV-Vis spectroscopy. The structural characteristics of the synthesized nanocomposites (NCs) were investigated by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FT-IR), and vibrating sample magnetometry (VSM) methods. In the proposed MSPE method, after optimizing the adsorption/desorption variables in MSPE and obtaining the optimum level of each variable (adsorption step: Vsample = 50 mL, contact time = 15 min, solution pH = 3, adsorbent mass = 0.05 g, ionic strength = 0.01 mol L-1; desorption step: eluent type and volume = ethanol (2 mL) for 10 min), the desorbed ethanolic solution was spectrophotometrically investigated in the UV-Vis region. The results confirm the developed MSPE method can be used for the preconcentration and determination of trace amounts of chlorpyrifos in the aqueous samples.