Grape Seed Extract Loaded Amino Functionalized SBA-15 Mesoporous Silica Nanoparticles as a potential drug delivery System: Antibacterial activity and release kinetics studies

Subject Areas : Biotechnological Journal of Environmental Microbiology

Mahdi Shahriarinour ¹ (Department of Microbiology, Faculty of Basic Sciences, Rasht Branch-Islamic Azad University, Rasht, Iran)
Faten Divsar ² (Department of Chemistry, Payame Noor University, PO BOX 19395-3697 Tehran, Iran)
Zahra Nazari Shad ³ (Department of Microbiology, Rasht Branch, Islamic Azad University, Rasht, Iran)

Received: 2023-07-13 Accepted : 2023-08-21 Published : 2022-10-01

Keywords: grape seed extract, S. aureus, E. coli, Antibacterial activity, SBA-15-mesoporous silica,

Abstract :

Mesoporous silica nanoparticles (MSNs) are used as efficient carrier candidate for the delivery of bioactive molecules, proteins, and drugs because of their properties such as safety, stability, biocompatibility, high surface area, and controllable pore sizes with narrow distribution and facile surface modification. MSNs demonstrate high encapsulation capability for both hydrophilic and hydrophobic drugs. In this study, SBA-15 MSN was synthesized by hydrothermal method in acidic media and functionalized by post-grafting method using 3-aminopropyltriethoxysilane. The efficiency of amino-functionalized SBA-15 MSN (SBA-15-NH2) was investigated for the loading and release of grape seed extract (GSE). The disc diffusion assay indicated GSE and GSE loaded on SBA-15-NH2 (SBA-15-NH2@GSE) at the concentration of 32 mg/ml extract per disc resulted in the inhibition zone of 24 mm and 15 mm for S. aurous and 22 mm and 13 mm for E. coli, respectively. The antibacterial effect improved by increasing the concentration of GSE loaded into SBA-15-NH2 from 2 to 32 mg/ml. These results indicate that SBA-15-NH2@GSE owns a strong antibacterial potential valuable for the pharmaceutical industry.