Dietary phospholipids (PL) levels on haematology parameters of beluga sturgeon (Huso huso) juveniles

Subject Areas : Breeding and Aquaculture Sciences Journal

Mahmoudreza Ebrahimnezhad ¹ , Cheroos Ibn Saad ² , Abdolmohammad Abedian Kenary ³

1 - Mazandaran Fishery Department, Babolsar, Iran
2 - Agriculture Department, Malaysia
3 - Fishery Department, Tarbiat Modares University, Noor, Iran

Received: 2017-05-24 Accepted : 2017-05-24 Published : 2017-02-19

Keywords: Nutrition, haematology, Phospholipids, Bleuga (Huso huso),

Abstract :

A study was carried out to determine the influence of dietary phospholipids (PL) levels on haematology parameters of beluga sturgeon (Huso huso) juveniles. Juveniles were fed formulated diet with four varying dietary levels of PL i.e. 0 (D1), 2 (D2), 4 (D3), and 6% (D4). At the end of the experimental period (56 days), results of the experiment also showed that there was a significant difference (P<0.05) observed in mean corpuscular hemoglobin concentration (MCHC) (g/dl).  Fish fed diet D2 (2% PL) had the highest MCHC with a value of 33.3 g/dl. There was no significant different (P>0.05) in other haematology parameters such as red blood cells (RBC), haematocrit (Hct), haemoglobin (Hb), mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), and white blood cells (WBC) in treated fish .  The highest RBC reading was found in fish fed diet D2 with numerical value of 1.07 (cells/l) for and the lowest value was found for fish fed diet D1 with a value of 0.86 (cells/l). Percent haematocrit readings ranged from the highest (fish fed D3) to the lowest (fish fed  D1) with values of 26.33 and 25.00% respectively. Haemoglobin (Hb) ranging from the highest (fish fed D2) to the lowest (fish fed D1) with values of 8.64 and 7.67 (g/dl) respectively.The measurement of differentiated white blood cell of Huso huso with varying dietary phospholipids levels such as lymphocytes, monocytes, neutrophils, and eosinophils  also showed that there were no significant differences (P>0.05) amongst the treatments.  Thehighest percentage amount of lymphocyte and monocyte were found in fish fed diet D2 with values of 71.67% and 3.67% respectively. While the highest readings for neutrophils, and eosinophils was found in fish fed diets D1 and D4 with values of 21.33% and 8.33%  respectively. In conclusion, the addition of  phospholipids in the juvenile’s beluga sturgeon no effect on hematological parameters.

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