Determination of Glyphosate Herbicide Median Lethal Concentration (LC50 96h) on Fry Common Carp (Cyprinus carpio)

Subject Areas : Breeding and Aquaculture Sciences Journal

Safoora Poshthopanah ¹ , mehdi mohammadalikhani ² , Saltanat najarlashgari ³ , seyyed pezhman hosseini shekarabi ⁴

1 - واحد لاهیجان
2 - تهران
3 - تنکابن، ایران
4 - تهران

Received: 2017-05-02 Accepted : 2017-05-02 Published : 2017-05-02

Keywords: herbicide, Glyphosate, Median lethal dose (LC50), Cprinus carpio,

Abstract :

This research was to determine the median lethal concentration (LC50 96h) Glyphosate that is used a lot on arable lands in order to determine the lethal concentration 50% of the fish population in 96 hours on 180 common carp (Cyprinus carpio) fry weighing 3 to 5 g. The experiments were performed as a resident and according to the standard methods (O.E.C.D) for 4 days (96 hours) and measured and controlled physical and chemical parameters of water such as pH, total hardness, dissolved oxygen and temperature. Also investigated the behavior of common carp larvae carefully during the test period. Preliminary experiments were conducted to find a range of lethality by releasing 10 common carp larvae per aquarium 20 liter with continuous aeration. The final test was done 5 treatments (concentrations of 65, 69.64, 74.61, 79.92 and 84.61 ppm) and three replications. The amount of LC10, LC50 and LC90 were calculated using Probit test. Acute toxicity of Glyphosate herbicide in common carps were calculated 68.83 ppm and based on the standards for pesticide toxicity was graded in the row pesticides with low toxicity.