The Genetical Structure of Caspian Sea Common Kilka (Clupeonella cultriventris caspia) by DNA mt Sequencing Method
Subject Areas : Breeding and Aquaculture Sciences Journal
Reza Shahriari
1
(واحد بابل)
Lida Shojaei
2
(واحد سوادکوه)
Faramarz Laloei
3
(ساری)
Asal Dabagh
4
(ساری)
Keywords:
Abstract :
This study evaluates the genetical structure of Caspian Sea Common Kilka (Clupeonella cultriventris caspia) during winter and Spring of 2013 by sequencing method. 30 samples were gathered from Mazandaran and Gilan fisheries ports and the fins samples lived in ethanol 96%. Then the genetical studies were done in biotechnology laboratory of Caspian Seas ecological research center. The DNA genomic samples of soft ports of the fish fin extracted by ammonium acetate method. The chain reaction of polymerase (PCR) was used by a pair of designed primer, on fish's DNA genomic. The abundant of Alles, the true number of Allelles, the observed and expected Heterozygosity, the genetic likeness and difference and the amount of Fs+ caluculated by Tajmia and Nei test of genetic software (Bio Edit and Arlequin). Dendrogram of genetic difference drawn by Mega 4 genetic software. Based on drawn phylogenetic tree, the samples of Guilan were divided from Mazandarn samples. The dendrogram of these fish showed that the D-Loop gene indentified the genetic structure of Caspian Seas Common Kilka and separates Mazandaran and Guilan populations directly. concentrations between fish muscles was on Aristichthys nobilis equal to 0.83 µg/g wet weight, that this amount and the amount of Copper in the muscle of Cyprinus carpio and Hypophthalmichthys molitrix were significantly different (P<0.05). The lowest amount of Copper in the muscle of fishes was on Hypophthalmichthys molitrix equal to 0.68 µg/g Wet weight, that this amount and the amount of Copper in the muscle of Aristichthys nobilis were significantly different (P<0.05). Hazard quotient in all samples was less than 0.1and so fish nutrition no risk in terms of the amount of Copper in the human body.