Histomorphology of Kidney and Hematological Indices of Nile tilapia, Oreochromis niloticus on Replacement of Blood Powder Instead of Fish Meal in the Diet
Subject Areas : Journal of Animal BiologyAmal Moshashai 1 , رحیم عبدی 2 * , rahim peyghan 3
1 - Department of Marine Biology, Faculty of Marine Science and Oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
2 - Department of Marine Biology, Faculty of Marine Science and Oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
3 - Department of Clinical Sciences and Excellence Center of Warm Water Fish Health and Diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Keywords: Histomorphology, Kidney, Blood powder, Blood Powder, Nile tilapia,
Abstract :
The present study aimed to investigate the effect of replacing blood powder with fish powder on the histomorphology of kidney tissue and some blood indices of Nile tilapia. After preparation, the fish were fed with designed diets for 8 weeks, including control, 25, 50, 75, and 100% blood powder. After this period, the fish were anesthetized and blood was collected from the caudal peduncle with a heparin syringe. While opening the abdominal cavity, five-millimeter samples were taken from the kidney. After placing in a 10% buffered formalin fixative solution and undergoing dehydration with alcohol, clarification with xylene, and paraffin blocking, sections were made to a thickness of 4-6 microns with a microtome. They were then stained with hematoxylin-eosin and finally studied with a light microscope. Results showed that in the group receiving blood powder, numerous complications such as hyaline accumulation, epithelial rupture, Bowman's capsule dilation, hyperemia, increased melanomacrophage centers, and glomerular necrosis and tubules were observed. As the percentage of blood powder in the diet increased, so did the severity of the lesions. With increasing the replacement of blood powder instead of fish powder in the diet, the number of red blood cells, the percentage of hematocrit, and the amount of hemoglobin show a decreasing trend. The number of lymphocytes, monocytes, and neutrophils increased with increasing levels of blood powder. There was a significant difference between treatments, especially at high levels (p < 0.05). According to the results of this study, blood powder can be tolerated and replaced up to 75% of the diet for this species.
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