Sonographic Evaluation of Ovulation Process in Native Breed Bitches Induced by Exogenous hCG and GnRH Administration at the Time of LH Surge with a 12-Hour Interval
Subject Areas : Journal of Animal Biologyerfan khodadadi 1 , Sakineh Asadzade vosta Kalaei 2 * , eisa Fallah 3
1 - Department of Veterinary, Babol Branch, Islamic Azad University, Babol, Iran
2 - Department of Animal Sciences, Ghaemshahr Branch, Islamic Azad University, Ghaemshahr, Iran
3 - Department of Veterinary, Babol Branch, Islamic Azad University, Babol, Iran
Keywords: Ultrasound, ovulation, dog, hCG, GnRH, LH,
Abstract :
The aim of this study was to investigate the sonographic effect of GnRH and hCG hormones on the process of ovulation and pregnancy in estrous bitches.Thirty – two native dogs were divided into two groups: a control group of 20 and a treatment group of 12 .After determining the sexual cycle of the dogs through blood tests and vaginal smears, as well as inducing estrus in those dogs that were in the anestrus phase, blood sampling was performed every 12 hours from the third day after the onset of vaginal bleeding to determine the LH peak. When the LH hormone concentration exceeded above 5 ng/ml, GnRH and hCG hormones were administered to all dogs in the treatment group. The process of ovulation and pregnancy diagnosis was carried out through ultrasound. The ovulation rate in the treated and control groups was 100% and 70%, respectively, which was statistically significant (p < 0.05). Additionally, ovulation occurred 48 hours after the LH peak in 83.3% of the dogs in the treated group and 60 hours after the LH peak in 16.7% of them. This was significantly different from the control group, where ovulation occurred 72 hours after the LH peak (p < 0.05). The pregnancy rate in the treated and control groups was 100% and 70%, respectively, and this difference was also statistically significant (p < 0.05). The results of this study indicated that the use of GnRH and hCG hormones can be employed to increase the ovulation rate, shorten the time to ovulation, and enhance the pregnancy rate in dogs.
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