The Effect of Hydroalcoholic Extract of the Aerial Parts of Malva Parviflora Plant on Growth Inhibition and Apoptosis Induction in Lung Cancer Cell Line A549
Subject Areas : Journal of Animal BiologyZohreh Valizadeh 1 * , Farideh Rahimee 2
1 - Department of Biology, Dezfoul Branch, Islamic Azad University, Dezfoul, Iran
2 - Department of Biology, Dezfoul Branch, Islamic Azad University, Dezfoul, Iran
Keywords: Malva parviflora, Lung cancer, A549 cell line, HFF cell line, Apoptosis,
Abstract :
lung cancer is one of the major cancers in the world that can be caused by the genetic changes in a series of important genes. Malva parviflora is a very valuable medicinal plant in traditional medicine. This plant is rich in vitamins A, B and C and is useful in treating respiratory infections. In this study, the effect of Malva hydroalcoholic extract on growth inhibition and apoptosis induction on A549 cancer cell line and HFF cell line was investigated. A549 and human HFF cell line were cultured in DMEM culture. Then, concentrations (100, 500 and 1000 µg/ml) of the extract were added to A549 and HFF cells in the culture medium, and after 24 hours, the MTT test was used to check the toxicity of the extract. To investigate the rate of proliferation or induction of apoptosis, the cells were stained with trypan blue. The results of the MTT test showed that the extract significantly decreased cell survival at concentrations of 100 (p ≤ 0.01), 500 and 1000 µg/ml (p ≤ 0.0001) in A549 cancer cells compared to the control group. The results of the trypan blue test showed that the extract caused a significant decrease in the growth rate of A549 cells compared to the HFF cell line at concentrations of 100 (p ≤ 0.05), 500 and 1000 µg/ml (p ≤ 0.0001). The proliferation rate of A549 cell line was statistically significant compared to HFF cell line at concentrations of 500, 100, 1000 µg/ml (p ≤ 0.05). results showed that the hydroalcoholic extract of Malva inhibited the proliferation of A549 cancer cells by inducing apoptosis. hydroalcoholic extract of Malva had a significant effect in reducing the growth rate of A549 cells compared to HFF cell line. hydroalcoholic extract of Malva with cytotoxic effects on A549 cancer cells caused the death of these cells through the induction of apoptosis.
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