Effect of 6 Weeks of Aerobic Training and Cinnamon Supplementation on Resistin Gene Expression in Slow Twitch Muscle Tissue of Male Wistar Rats Fed with High Fat Diet

Subject Areas : Journal of Animal Biology

nazanin abdollahzadeh ¹ (Department of Physical Education and Sport Sciences, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran)
yaser kazemzadeh ² (Department of Physical Education and Sport Sciences, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran)
saeid sedaghati ³ (Department of Physical Education and Sport Sciences, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran)
Sanaz Mirzayan Shanjani ⁴ (Department of Physical Education and Sport Sciences, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran)
zahra charbaghi ⁵ (Department of Physical Education and Sport Sciences, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran)

Received: 2024-04-07 Accepted : 2024-04-25 Published : 2024-08-06

Keywords: Aerobic training, Resistin gene expression, Fast twitch muscle tissue, Slow twitch muscle tissue, High fat diet.,

Abstract :

The purpose of this research is to determine the effect of 6 weeks of aerobic training and cinnamon supplementation on the expression of the resistin gene in the slow twitch muscle tissue of male Wistar rats fed with a high-fat diet. The statistical sample of the research consists of 50 two-month-old male Wistar rats, which were randomly divided into five groups, and each group consisted of ten rats. The mice had daily access to 20 grams of pellets and 10-12 ml of water per 100 grams of body weight. The groups receiving high-fat food used high-fat food emulsion daily of 1.5 mg per kilogram of body weight. The aerobic exercise group participated in the aerobic exercise program on the electronic treadmill five days a week for six weeks. The cinnamon extract group received 200 mg per kilogram of body weight for 6 weeks. To analyze the data, dependent t-tests, one-way analysis of variance and, Tukey's follow-up were used. The results showed that the effects of a high-fat diet, combination of high-fat diet and aerobic exercise, the combination of high-fat diet and cinnamon, and a combination of high-fat diet, aerobic exercise and cinnamon on resistin gene variables in slow-twitch muscle tissue are significant (p ≥ 0.05). As a result, considering the optimal and favorable effects of taking cinnamon extract along with performing aerobic exercise on body weight and resistance gene expression and adjusting the adverse effects of consuming a high-fat diet, it is recommended to use cinnamon extract along with performing aerobic exercise.

References:

1. Ahmadizadeh S., Salehi M., Hedayati M., Norshahi M. 2009. The effect of intense resistance exercise load on adipokines and insulin resistance index. Journal of Exercise Physiology, (7):143-158 [In Persian].
2. Badalzadeh. R., Shaghaghi. M., Mohammadi M., Dehghan. G., Mohammadi Z. 2014.The effect of cinnamon extract and long-term aerobic training on heart function, biochemical alterations and lipid profile following exhaustive exercise in male rats. Advanced Pharmaceutical Bulletin, 4(2):515-520.
3. Bakhoda H., Neati N., Bagherpour T. 2018. research entitled the effect of six weeks of aerobic exercise and cinnamon extract on interleukin-1 beta gene expression in the muscle tissue of male mice fed with a high-fat diet. Journal of Animal Biology, 12(2):13-22 [In Persian].
4. Bani Talebi Dehkordi S., Faramarzi M., Rahimi M., Sadeghi A. 2022 Examining changes in the resting levels of some inflammatory indicators, muscle damage, aerobic and anaerobic capacity of elite beach soccer players during the Premier League season. Journal of Applied Studies of Biological Sciences in Sports, 22:87-97 [In Persian].
5. Eftekhari E., Zafari A., Gholami M. 2016. Physical activity, lipid profiles and leptin, The Journal of Sports Medicine and Physical Fitness, 56(4):465-469 [In Persian].
6. Farrokh.A Avdel E., Naji T. 2019. Review of Leptin and Resistin. Journal of Laboratory Diagnosis, 176:38-42 [In Persian].
7. Irandoost T., Abdi A., Abbasi Daloii A. 2018. The effect of aerobic exercise with the addition of cinnamon extract on resistance and sugar indices in mice with insulin resistance, Shahid Beheshti University. Journal of Physiology of Exercise and Physical Activity, 12(1):43-57 [In Persian].
8. Jafari A., Rasouli H., Zarvar M. 2016. The study of the effect of a course of resistance training and consumption of milk thistle plant extract on leptin and adiponectin plasma in overweight men. Sports Physiology Journal, 8(31):93-107 [In Persian].
9. Mirzendedel Z., Attarzadeh Hosseini S.R., Bijeh N., Raouf Saeb A.A. 2020. Effects of Combined Training on Progranulin, Glycosylated Hemoglobin, and Insulin Resistance in Overweight and Obese Women with Type 2 Diabetes. Iranian Journal of Endocrinology and Metabolism, 22(4):316-327 [In Persian].
10. Nasery L., Zafari A., Banayifar A. 2015. The response of Leptin and lipid parameters related to an aerobic exercise among young athlete and non- athlete women. Indian Journal of Fundamental and Applied Life Sciences, 5(S3):788-798.
11. Ossanloo P., Najar L., Zafari A. 2012. The effects of combined training (aerobic dance, step exercise and resistance training) on body fat percent and lipid profiles in sedentary females of ALZAHRA University. European Journal of Experimental Biology, 2(5):1598-1602. [In Persian].
12. Ranjbar R., Ahmadizad S., Khoshniyat Niko M., Salimi A. 2017. The effect of endurance training with fasting and a non-training period on plasma levels of adipo-nectin and insulin resistance index in inactive men. Journal of Exercise Physiology, (15):163-180 [In Persian].
13. Rigi A., Bagherpour T., Nemati N. 2019. The effect of six weeks of aerobic exercise and cinnamon extract on the expression of interleukin-6 gene in adipose tissue of male mice fed with a high-fat diet, Journal of Animal Biology, 13(2) 60- 49 [In Persian].
14. Roussel AM., Hininger I., Benaraba R., Ziegenfuss T.N., Anderson R.A. 2009. Antioxidant effects of a cinnamon extract in people with impaired fasting glucose that are overweight or obese. Journal of the American College of Nutrition, 28(1):16-21.
15. Safarzadeh A. R., Gharakhanlou R., Hedayati M., Talebi-Garakani E. 2012. The effect of 4 weeks resistance training on serum vaspin, Il-6, CRP and TNF-Α concentrations in diabetic rats. Iranian Journal of Endocrinology and Metabolism, 14(1):68-74 [In Persian].
16. Sartorius T., Peter A., Schulz N., Drescher A., Bergheim I., Machann J., Schick F., Siegel-Axel D., Schürmann A., Weigert C., Häring H.U., Hennige A.M. 2014. Cinnamon extract improves insulin sensitivity in the brain and lowers liver fat in mouse models of obesity. PLoS One, 9(3):e92358.
17. Shalaby M.A., Saifan H.A. 2014. some pharmacological effects of cinnamon and ginger herbs in obese diabetic rate. Journal of Intercultural Ethnopharmacology, 3(4):144-149 [In Persian].
18. Lee S.M., Lee M.C., Bae W.R., Yoon K.J., Moon H.Y. 2022. Muscle fiber type-dependence effect of exercise on genomic networks in aged mice models. Aging, 14:3337-3364.
19. Taghavi Moghadam A., Shiravand M. 2016. The effect of 8 weeks of resistance training along with cinnamon supplementation on glycemic indices of obese men, Nurse and Doctor in War Quarterly, 4(12):133-139 [In Persian].
20. Valizadeh A., Akbarnejad A., Yari M., Rajabi A., Bavardi Moghadam E. 2017. The effect of a course of aerobic training on the amount of resistin, glycosylated hemoglobin, insulin resistance index and some cardiovascular risk factors in obese women with type 2 diabetes. Sports Biological Sciences, 3(38):263-282 [In Persian].