Effect of Hydroalcoholic Extract of Trachyspermum copticum on Anxiety in Male Rats

Subject Areas : Journal of Animal Biology

Z. Mohammadi Manesh ¹ , Nasrin Heidarieh ²

1 - Department of Biology, Qom Branch, Islamic Azad University, Qom , Iran
2 - Department of Biology, Qom Branch, Islamic Azad University, Qom , Iran

Received: 2018-04-21 Accepted : 2018-04-21 Published : 2018-02-20

Keywords: Anxiety, elevated plus maze, hydroalcoholic extract, Trachyspermum copticum, Male rats,

Abstract :

Anxiety is a common disorder of unknown origin is associated with an unpleasant feeling. According to the anxiolytic effects TC traditional medicine, Trachyspermum copticum effect on anxiety male Wistar rats was investigated. T. copticum seed after detection by the university herbarium, hydro-alcoholic extract was extracted by Soxhlet and the extract powder was prepared using smart legitimate. Male Wistar rats (230 ± 20 gr) were placed. In groups intact, recipient of the DMSO (solvent extraction) (0.3 ml), received extract in different doses of 50, 25, 15 mg/kg. All injections were performed intraperitoneally. Half an hour after injection, anxiety was measured using the elevated plus maze. Indicators of anxiety for 5 min, including time spent and number of entries into the open arm was recorded. Data were analyzed by ANOVA statistical tests and Tukey test. The group receiving the extract solvent (DMSO) compared to intact in time spent and number of entries into the open arm did not show a significant difference. The group receiving a dose of 15 mg/kg compared to the group receiving DMSO in time spent and number of entries into the open arm showed a significant increase. T. copticum hydroalcoholic extract has anxiolytic effects in low doses.

References:

 

1. Aboujaoude E., Barry J., Gamel N., 2009. Memantine augmentation in treatment-resistant obsessive-compulsive disorder: an open-label trial. Journal of Clinical Psychopharmacology, 29(1): 51-55.


2. Akbarinia A., Sefidkon F., Ghalavand A., Tahmasebi Z., Sharifi A., 2005. Chemical composition of seed essence of Trachyspermum copticum produced in Qazvin Province. Journal of Qazvin University of Medical Sciences, 36: 22-24.


3. Alizadeh M., Hoseini M., Shojaezadeh D., Rahimi A., Arshinji M., Rohani H., 2012. Assessing anxiety, depression and Psychological wellbeing status of urban elderly under represent of Tehran metropolitan city. Salmand Iranian Journal of Ageing, 7(3): 66-73.


4. Arman M., 2014. The comparison of depression, anxiety and stress between active and inactive old women in Isfahan. Journal of Rehab Medicine, 3(3): 82-8.


5. Babazadeh T., Sarkhoshi R., Bahadori F., Moradi F., Shariat F., Sherizadeh Y., 2016. Prevalence of depression, anxiety and stress disorders in elderly people residing in Khoy, Iran (2014-2015). Journal of Anal Research and Clinical Medicine, 4(2): 122-8.


6. Bahmani K., Izadi-Darbandi A., Sadat-Noori S.A., 2013. Evaluation of the contents and essential oil constituents of some ecotypes of Iranian fennel. Journal of Crops Improvement, 15(4): 13-24.

1. Banaeipour Z., Rostami S., Zarea K., Cheraqian B., 2016. The Prevalence of Anxiety and its related Factors among School-age Children in South West of Iran. International Journal of Pediatrics, 4(6): 2019-2025.


2. Blicher J.U., Near J., Naess-Schmidt E., 2015. GABA levels are decreased after stroke and GABA changes during rehabilitation correlate with motor improvement. Neurorehabilitation and Neural Repair, 29: 278-286.


3. Boskovic M., Vovk T., Kores-Plesnicar B., Grabnar I., 2011. Oxidative stress in schizophrenia. Current Neuropharmaco logy, 9: 301-312.


4.  Cases J., Ibarra A., Feuillere N., Roller M., Sukkar S.G., 2011. Pilot trial of Melissa officinalis L. leaf extract in the treatment of volunteers suffering from mild-to-moderate anxiety disorders and sleep disturbances. Medical Journal of Nutrition Metabolism, 4(3): 211-218.


5.  Celano M., Maggisano V., De Rose R.F., Bulotta S., Maiuolo J., Navarra M., Russo D., 2015. Flavonoid fraction of Citrus reticulata juice reduces proliferation and migration of anaplastic thyroid carcinoma cells. Nutrition and Cancer, 67: 1183-1190.


6.  El-Merahbi R., Löffler M., Mayer A., and Sumara G., 2015. The roles of peripheral serotonin in metabolic homeostasis. FEBS Letters, 589(15): 1728-1734.


7.  Ferlazzo N., Visalli G., Cirmi S., Lombardo G.E., Lagana P., Di Pietro A., Navarra M., 2016. Natural iron chelators: Protective role in a549 cells of flavonoids-rich extracts of citrus juices in Fe3+-induced oxidative stress. Environmental Toxicology and Pharmacology, 43: 248-256.


8.  Goudarzi G.R., Saharkhiz M.J., Sattar M., Zomorodian K., 2011. Antibacterial activity and chemical composition of Ajowan (Carum copticum) essential oil. Journal of Medical Plant Research, 13: 203-208.


9.  Hanrahan J.R., Chebib M., Johnston G.A., 2011. Flavonoid modulation of GABA (A) receptors. British Journal of Pharmacology, 163: 234-245.


10.  Hiu T., Farzampour Z., Paz J., 2016. Enhanced phasic GABA inhibition during the repair phase of stroke: a novel therapeutic target. Brain: A Journal of Neurology, 139: 468-480.


11.  Ibarra A., Feuillere N., Roller M., Lesburgere E., Beracochea D., 2010. Effects of chronic administration of Melissa officinalis L. extract on anxiety like reactivity and on circadian and exploratory activities in mice. Phytomedicine, 17(6): 397-403.


12.  Impellizzeri D., Cordaro M., Campolo M., Gugliandolo E., Esposito E., Benedetto F., Cuzzocrea S., Navarra M., 2016. Anti-inflammatory and antioxidant effects of flavonoid-rich fraction of bergamot juice (BJe) in a mouse model of intestinal ischemia/reperfusion injury. Frontiers in Pharmacology, 7: 203.


13.  Isidora S., Mijatovic V., Petkovic S., Skrbic B., Bozin B., 2012. The influence of essential oil of aniseed (Pimpinella anisum, L.) on drug effects on the central nervous system. Fitoterapia, 48: 1466-1473.


14.  Kumar D, Bhat Z, Kumar V, Shah M., 2011. Nature: Anxiolytics in the Lap of Nature. WebmedCentral Pharmaceutical Sciences, 2(9): WMC002140


15.  Mellon S.H., Li D., 2002. Griffin, neurosteroids: biochemistry and clinical significance. Trends in Endocrinology and Metabolism, 13(1): 35-43.

 Neufeld K.M., Kang N., Bienenstock J., Foster J.A., 2011. Reduced anxiety-like behavior and central neurochemical change in germ-free mice. Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society, 23(3): 255-264

1.  Niksokhan M., Hedarieh N., Najafifard M., Najafifard M.., 2015. Effect of hydroalcoholic extract of Pimpinella anisum seed on anxiety in male rat. Journal of Gorgan University of Medical Sciences, 16(4): 28-33.


2.  Perez-Cano F.J., Massot-Cladera M., Rodriguez-Lagunas M.J., Castell M., 2014. Flavonoids affect host-microbiota crosstalk through TLR modulation. Antioxidants, 3(4): 649-670.


3.  Pérez-Mato M., Ramos-Cabrer P., Sobrino T., Blanco M., Ruban A., Mirelman D., Menendez P., Castillo J., Campos F., 2014. Human recombinant glutamate oxaloacetate transaminase 1 (GOT1) supplemented with oxaloacetate induces a protective effect after cerebral ischemia. Cell Death and Disease, 5:e992.


4.  Risitano R., Currò M., Cirmi S., Ferlazzo N., Campiglia P., Caccamo D., Ientile R., Navarra M., 2014. Flavonoid fraction of bergamot juice reduces LPS-induced inflammatory response through SIRT1-mediated NF-B inhibition in THP-1 monocytes. PloS ONE, 9: e107431.


5.  Shikov A.N., Pozharitskaya O.N., Makarov V.G., Wagner H., Verpoorte R., Heinrich M., 2014. Medicinal plants of the Russian Pharmacopoeia; their history and applications. Journal of Ethnopharmaco logy, 154: 481-536.


6.  Umar S., Asif M., Sajad M., Ansari M., Hussain U., Ahmadi W., 2012. Anti-inflammatory and antioxidant activity of Trachyspermum ammi seeds in collagen induced arthritis in rats, International Journal of Drug Development and Research, 4(1): 210-219.


7.  Wadikar D.D., Premavalli K.S., 2012. Ajowan (Trachyspermum ammi) munch: A shelf stable ready-to-eat appetizer, its development and storage. International Food Research Journal, 19(1): 321-325.


8.  Waszczuk M., Zavos H., 2013. Genetic and environmental influences on relationship between anxiety sensitivity and anxiety subscales in children. Journal of Anxiety Disorders, 27:475-484.