Effect of Hydroalcoholic Extract of Trachyspermum copticum on Anxiety in Male Rats

Subject Areas : Journal of Animal Biology

Z.  Mohammadi Manesh ¹ (Department of Biology, Qom Branch, Islamic Azad University, Qom , Iran)
Nasrin  Heidarieh ² (Department of Biology, Qom Branch, Islamic Azad University, Qom , Iran)

Received: 2018-04-21 Accepted : 2018-04-21 Published : 2018-02-20

Keywords: Anxiety, elevated plus maze, hydroalcoholic extract, Trachyspermum copticum, Male rats,

Abstract :

Anxiety is a common disorder of unknown origin is associated with an unpleasant feeling. According to the anxiolytic effects TC traditional medicine, Trachyspermum copticum effect on anxiety male Wistar rats was investigated. T. copticum seed after detection by the university herbarium, hydro-alcoholic extract was extracted by Soxhlet and the extract powder was prepared using smart legitimate. Male Wistar rats (230 ± 20 gr) were placed. In groups intact, recipient of the DMSO (solvent extraction) (0.3 ml), received extract in different doses of 50, 25, 15 mg/kg. All injections were performed intraperitoneally. Half an hour after injection, anxiety was measured using the elevated plus maze. Indicators of anxiety for 5 min, including time spent and number of entries into the open arm was recorded. Data were analyzed by ANOVA statistical tests and Tukey test. The group receiving the extract solvent (DMSO) compared to intact in time spent and number of entries into the open arm did not show a significant difference. The group receiving a dose of 15 mg/kg compared to the group receiving DMSO in time spent and number of entries into the open arm showed a significant increase. T. copticum hydroalcoholic extract has anxiolytic effects in low doses.

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