The Effect of Fe2O4Zn Nanoparticles on Kidney Tissue and Blood Biochemical Factors in Wistar Male Rats

Subject Areas : Journal of Animal Biology

D. Rahmani ¹ , Z. Hooshmandi ² , Ferdowsi Sh. ³

1 - Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
2 - Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
3 - Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Kurdestan Blood Transfusion Research, Sanandaj, Iran

Received: 2017-06-17 Accepted : 2017-06-17 Published : 2017-05-22

Keywords: Rat, kidney, Creatinine, Uric acid, Fe2O4Zn Nanoparticle,

Abstract :

Nanoparticles should be investigated under the laboratory conditions for using in treatment of diseases. This study aimed to investigate the effects of nanoparticles of iron-zinc (Fe2O4Zn) on the factors kidney male rats Wistar. In this experimental study, 24 male Wistar rats were randomly divided into 3 groups containing two experimental and one control group. The control group received 0.5 ml of saline solution but others groups received 0.5 ml of iron nanoparticle with concentration of 100 ppm and 200 ppm, respectively for 7 consecutive days injected intraperitoneally. On the second, seventh and fourteenth day after treatment, blood from the corner of the eyelids was done with capillary tube. Urea, uric acid, and creatinine were measured using ELISA method. Results showed that urea and uric acid levels in the seventh and fourteenth days after treatment at a dose of 100 ppm and 200 ppm were decreased. The creatinine values ​​at 2, 7 and 14 d after treatment 100 ppm and 200 ppm were increased. At a low dose, nanoparticles sediment in the connective tissue and cytoplasm of proximal and distal renal cell and at high doses of nanoparticles, deposit is made in cells of the proximal tubule and in the central part of kidney. Levels of urea and uric acid were reduced and creatinine was increased. The results of histological showed nanoparticle deposition in the kidney. The application of Fe2O4Zn nanoparticles in the biological system had no stable and long-term toxic effects on the animal body.

References:

1. Amara S., Slama I. B., Mrad I., Rihane N., Khemissi W., El Mir L., Sakly M., 2014. Effects of zinc oxide nanoparticles and/or zinc chloride on biochemical parameters and mineral levels in rat liver and kidney. Human andExperimental Toxicology, 0960327113510327.

2. Azadeh N., Hooshmandi Z.,  Setorki M., 2015. Effect of Fe4NiO4Zn Nanoparticles on Serum Urea-Uric Acid and Creatinine in Male Rat. Medical Journal of Tabriz University of Medical Sciences and Health Services,37(3): 6-11.

3. Chen Z., Meng H., Xing G., Chen C., Zhao Y., Jia G., Zhao F., 2006. Acute toxicological effects of copper nanoparticles in vivo. ToxicologicalLetters, 163(2): 109-120.

4. Doudi, M., & Setorki, M. (2014. Effect of dioxid-titanium nanoparticles on function and tissue of kidney. Urmia Medical Journal,25(8): 684-692.

 

5. Hillyer J.F., Albrecht R.M., 2001. Gastrointestinal persorption and tissue distribution of differently sized colloidal gold nanoparticles. Journal of Pharmaceutical Sciences,90(12): 1927-1936.

6. Kumari M., Rajak S., Singh S.P., Murty U. S., Mahboob M., Grover P., Rahman M.F., 2013. Biochemical alterations induced by acute oral doses of iron oxide nanoparticles in Wistar rats. Drug and Chemical Toxicology,36(3): 296-305.

7. Noori A., Amiri G. R., Taj B., Isfahani M. N., Taj S., Valiani A., 2012. The Effect of Magnetic Iron Oxide Nanoparticles on Mice Liver and Kidney. Journal of Kerman University of Medical Sciences,19(3): 243-252.

8. O’Neill M., Hutchison G., 2008. The effect on nanoparticale exposure on male reproductive function. Nanotoxicology,10: 2261-2268.

9. Tang, F., Li, L., Chen, D., 2012. Mesoporous silica nanoparticles: synthesis, biocompatibility and drug delivery. Advanced Materials, 24(12): 1504-1534.