The Teratogenic Effects Pesticide of Chlorpyrifos on the Development of BALB/c Mouse Embryos 3Th to 6Th Days of Pregnancy

Subject Areas : Journal of Animal Biology

S. Rastgar Gharahshiran ¹ , P. Torabzadeh ² , S. Saffari ³

1 - Department of Biology, Karaj Branch, Islamic Azad University, Karaj, Iran
2 - Department of Biology, Karaj Branch, Islamic Azad University, Karaj, Iran
3 - Department of Biology, Karaj Branch, Islamic Azad University, Karaj, Iran

Received: 2017-06-17 Accepted : 2017-06-17 Published : 2017-05-22

Keywords: Chlorpyrifos, Teratogen, Mouse embryo, Agricultural pesticides,

Abstract :

Chlorpyrifos is one of organophosphate insecticides and had studied in recent years because it has many complications devastating on skin, nervous, respiratory and digestive systems, but its effects on the embryo especially during pregnancy has not been reviewed. Therefore, in study the teratogenic effects pesticide of Chlorpyrifos on the development of BALB/c mouse embryos 3rd to 6thd of pregnancy. In this study, 50 female BALB/c mouse were randomly divided into 6 equal groups a control group (non-injection: 5 mice) and witness (injection of saline: 5 mice) and 4 experimental groups (each group: 10 mice). A lethal dose LD50 was determined in condition of 25.32 ml/kg.bw in vivo and selected dose for injection 0.4 ml/kg.bw. Injection was done on the 3rd to 6th d of pregnancy days by enema. Then the mouse was sacrificed on day 15 of pregnancy. For security of results above experiences was repeated 2 times. Data were checked with SPSS 17 software by percentage. After a comparative study at this dose has been observed a significant increase, extrahepatic, exencephaly, syndactyly, having a defect in dynamic organs (legs and hands), extensive bleeding in whole body and exophthalmia were compared with control and witness groups.According to the findings, negative effects of Chlorpyrifos use as pesticides in agricultural products. On the mouse embryos, it is recommended to protect the environment and human health, especially pregnant women use of non-chemical methods to control pests.

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