Protective Effect of Royal Jelly on Methotrexate Infertility in Female Mice

Subject Areas : Journal of Animal Biology

Hossein Bakhoda ¹ (Department of Physical Education and Sport Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran)
Nematollah Nemati ² (Department of Physical Education and Sport Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran)
Tahereh Bagherpoor ³ (Department of Physical Education and Sport Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran)

Received: 2019-08-11 Accepted : 2020-02-19 Published : 2020-02-20

Keywords: royal jelly, Ovary, NMRI mouse, Methotrexate,

Abstract :

Methotrexate is a chemotherapy drug that is widely used in neoplastic disorders and ectopic pregnancy. In previous research, the toxicity of methotrexate has been reported on the genital tract. The properties of Royal jelly such as impotence improvement, and antioxidant and anticancer function have been reported. Therefore, in the present research, the effect of Royal jelly on ovarian cells in NMRI female mice treated with methotrexate was investigated. In this study, 70 female mice were divided into: negative control (without injection), positive control (received 0.5 mg/kg methotrexate), sham, and three experimental groups (T1, T2, and T3 received methotrexate plus 25, 50 and 100 mg/kg Royal jelly). Intraperitoneal injection was performed for 8 days. After histological processing and staining, the ovarian tissue, primary, secondary and graafian follicles, yellow body, as well the number of generation was investigated. Data were analyzed by Tukey test and one way ANOVA. Results showed there was a significant decrease in the number of generation in positive control, but the number of generation in experimental groups returned to the initial state. Regarding the duration of fertility, there was a significant decrease in the fertility rate in T1 and T3 compared to positive control group. The results of estrogen hormone showed a significant decrease in positive control group compared to negative control and increased significantly in T1 and T2. The number of primary, secondary, and graafian follicles in T 2 and T3 showed a significant increase (P<0.001). According to the present study, it was found that with further investigations, the appropriate dose of Royal jelly could act as a protective agent against the side effects of methotrexate and improve fertility.

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