Response of mTOR / P70S6K myokines to eccentric and concentric physical activity in healthy young men

Subject Areas : Journal of Animal Biology

Amirhossein Niroumand ¹ (Department of Sport Physiology, Central Tehran branch, Islamic Azad University, Tehran, Iran)
Ardavan Jamialahmadi ² (Department of Sport Physiology, Central Tehran branch, Islamic Azad University, Tehran, Iran)
Hassan Matin Homaei ³ (Department of Sport Physiology, Central Tehran branch, Islamic Azad University, Tehran, Iran)

Received: 2020-08-27 Accepted : 2023-12-07 Published : 2024-03-05

Keywords: Eccentric contraction, Concentric contraction, mTOR / P70S6K,

Abstract :

The purpose of this study was to evaluate the response of mTOR/P70S6K myokines to eccentric and concentric physical activity in healthy young men. 10 healthy men were randomly divided into each group (concentric group of 5 people - eccentric group of 5 people). Isokinetic contraction protocols included eccentric and concentric knee extensions with a maximum strength and angular velocity of 60 °/s. The torques set for each subject were considered the same in order to match the load in both protocols and the reciprocating speed was 60 degrees per second. At the beginning and end of the study, a biopsy was performed of lateral extensor muscle tissue. Real Time PCR technique was used to evaluate the expression of mTOR/P70S6K gene. To analyze the data of this study, dependent t-test and analysis of covariance were used at a significance level of 0.05. The results showed that the changes within the mTOR group were significant in the concentric group but not significant in the eccentric group. Also, the intragroup changes of P70S6K in the eccentric and concentric groups were not significant (P≥0.05). Also, intergroup changes showed no difference between the two groups in both variables (P≥0.05). It seems that the lack of significant changes in the desired variables is due to the lack of sufficient training pressure to stimulate the increase of mTOR/P70S6K. And according to the review of the answer, it seems that in the discussion of compatibility, different results are obtained that need to be examined.

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