Protective Effect of Coenzyme Q10 on Rat Sperm and Testicular Tissue after Exposure to Diazinon

Subject Areas : Journal of Animal Biology

Sima Ebadi Naftchali ¹ (Department of Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran)
Ramezan Khanbabaei ² (Department of Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran)
Abasali Dehpour Jouybari ³ (Department of Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran)
Roya Bishekolaei ⁴ (Department of Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran)
Esmaeil Fattahi ⁵ (Department of Biology, Amol Branch, Islamic Azad ayatollah Amoli University, Amol, Iran)

Received: 2023-07-16 Accepted : 2023-08-05 Published : 2024-02-20

Keywords: Infertility, Oxidative stress, diazinon, Co-enzyme Q10, Tissue damage,

Abstract :

Diazinon (DZN) is an organophosphate insecticide that causes a wide range of pathological effects on the male reproductive system, disturbances in sperm production and quality, and fertility problems. This study investigated the protective effect of coenzyme Q10 treatment on sperm quality, oxidant/antioxidant system, and histopathology of testicular tissue in male rats after exposure to diazinon. This experimental study was carried out on 16 adult male Wistar albino rats with an approximate weight of 150 to 230 gr, which were obtained from the animal care center.The animals were randomly divided into four groups (n=4) and were tested by intraperitoneal injection for 30 days which including the diazinon exposure group (50 mg/kg of diazinon dissolved in sesame oil intraperitoneally injected), the coenzyme Q10 and diazinon exposure group (50 mg/kg of diazinon and 10 mg/kg of coenzyme Q10 dissolved in sesame oil intraperitoneally injected), group of coenzyme Q10 (10 mg/kg of coenzyme Q10 dissolved in sesame oil intraperitoneally injected), and the control group (1.5 mg/kg of sesame oil intraperitoneally injected). After treating coenzyme Q10 with diazinon, a significant increase in the number and motility of sperm and a decrease in sperm mortality were observed (P<0/05). Exposure to diazinon with coenzyme Q10 treatment was able to decrease the level of malondialdehyde (MDA) and increase the activity of superoxide dismutase (SOD) compared to the diazinon group (P<0/05). Simultaneous exposure to diazinon and coenzyme Q10 treatment led to a decrease in testicular histopathological damage after exposure to diazinon. It seems that coenzyme Q10 can have a good protective effect against oxidative damage in the male reproductive system caused by exposure to diazinon.

References:

1. Abdulidha N.A., Jaccob AA., AL‑Mozie M.S.G. 2020. Protective effects of Co-Q10, Ginkgo biloba, and l-carnitine on brain, kidney, liver, and endocrine system against sub-acute heavy metals toxicity in male rats. Toxicology and Environmental Health Sciences, 12:331-341.


2. Adamkovicova M. Toman R., Martiniakova M., Omelka R., Babosova R., Krajcovicova V., Grosskopf B., Massanyi P. 2016. Sperm motility and morphology changes in rats exposed to cadmium and diazinon. Reproductive Biology and Endocrinology, 14(1):1-7.


3. Akbari, S., Amiri F.T., Naderi M., Shaki F., Seyedabadi M. 2022. Sodium arsenite accelerates D-galactose-induced aging in the testis of the rat: Evidence for mitochondrial oxidative damage, NF-kB, JNK, and apoptosis pathways. Toxicology, 470:153148.


4. Alahmar A.T., Sengupta P. 2021. Impact of coenzyme Q10 and selenium on seminal fluid parameters and antioxidant status in men with idiopathic infertility. Biological Trace Element Research, 199(4):1246-1252.


5. Alluwaimi, A.M., Hussein Y. 2007. Diazinon immunotoxicity in mice: modulation of cytokines level and their gene expression. Toxicology, 236(1-2): 123-131.


6. Awad M.A. 2022. Hormonal and histological study of the effect of co enzyme Q10 on male reproductive system of wister rats exposed to cadmium chloride. Journal of Madenat Alelem University College, 14(1):216-227.


7. Beharry K.D., Cai C.L., Henry M.M., Chowdhury S., Valencia G.B., Aranda J.V. 2017. Co-Enzyme Q10 and n-3 polyunsaturated fatty acid supplementation reverse intermittent hypoxia-induced growth restriction and improved antioxidant profiles in neonatal rats. Antioxidants, 6(4):103.


8. Čolović M., Krstić D., Petrović S., Leskovac A., Joksić G., Savić J., Franko M., Trebse P., Vasić V. 2010. Toxic effects of diazinon and its photodegradation products. Toxicology letters, 193(1):9-18.


9. ElMazoudy R.H., Attia A.A. 2012. Endocrine-disrupting and cytotoxic potential of anticholinesterase insecticide, diazinon in reproductive toxicity of male mice. Journal of Hazardous Materials, 209:111-120.


10. Gaines T.B. 1960. The acute toxicity of pesticides to rats. Journal of Occupational and Environmental Medicine, 2(7): 352.


11. Güleş Ö., Kum Ş., Yıldız M., Boyacıoğlu M., Ahmad E., Naseer Z., Eren Ü. 2019. Protective effect of coenzyme Q10 against bisphenol-A-induced toxicity in the rat testes. Toxicology and Industrial Health, 35(7):466-481.


12. Harchegani A.B., Rahmani A., Tahmasbpour E., Kabootaraki H.B., Rostami H., Shahriary A. 2018. Mechanisms of diazinon effects on impaired spermatogenesis and male infertility. Toxicology and Industrial Health, 34(9):653-664.


13. Jalali, A.S., Hasanzadeh S., Malekinejad H. 2012. Achillea millefolium inflorescence aqueous extract ameliorates cyclophosphamide-induced toxicity in rat testis: stereological evidences. Chinese Journal of Natural Medicines, 10(4):247-254.


14. Kouhestani S., Jafari A., Babaei P. 2018. Kaempferol attenuates cognitive deficit via regulating oxidative stress and neuroinflammation in an ovariectomized rat model of sporadic dementia. Neural Regeneration Research, 13(10):1827-1832.


15. Leong C.T., D'Souza U.J., Iqbal M., Mustapha Z.A. 2013. Lipid peroxidation and decline in antioxidant status as one of the toxicity measures of diazinon in the testis. Redox Report, 18(4):155-164.


16. Mousavi Pourgohar Z., Kianifard D., Khalilzadeh E. 2016. The Microscopic and Ultra Structural Study of Testicular Tissue Following Time Dependent Administration of Methylphenidate in Adult Rats. Journal of Ilam University of Medical Sciences, 24(4):83-94.


17. Mutch E., Williams F.M. 2006. Diazinon, chlorpyrifos and parathion are metabolised by multiple cytochromes P450 in human liver. Toxicology, 224(1-2):22-32.


18. Naderi, M., Ahangar N., Badakhshan F., Ghasemi M., Shaki F. 2021. Zinc and selenium supplement mitigated valproic acid-induced testis toxicity by modulating the oxidative redox balance in male rats. Anatomy and Cell Biology, 54(3):387-394.


19. Nadjarzadeh A., Shidfar F., Amirjannati N., Vafa M.R., Motevalian S.A., Gohari M.R., Nazeri Kakhki S.A., Akhondi M.M., Sadeghi M.R. Effect of Coenzyme Q10 supplementation on antioxidant enzymes activity and oxidative stress of seminal plasma: a double‐blind randomised clinical trial. Andrologia, 2014. 46(2):177-183.


20. Najafi, M., Cheki M., Amini P., Javadi A., Shabeeb D., Eleojo Musa A. 2019. Evaluating the protective effect of resveratrol, Q10, and alpha-lipoic acid on radiation-induced mice spermatogenesis injury: A histopathological study. International Journal of Reproductive BioMedicine, 17(12):907.


21. O’Flaherty C., Matsushita-Fournier D. 2017. Reactive oxygen species and protein modifications in spermatozoa. Biology of Reproduction, 97(4):577-585.


22. Pina-Guzman B., Solis-Heredia M., Quintanilla-Vega B. 2005. Diazinon alters sperm chromatin structure in mice by phosphorylating nuclear protamines. Toxicology and Applied Pharmacology, 202(2):189-198.


23. Safarinejad M.R. 2009. Efficacy of coenzyme Q10 on semen parameters, sperm function and reproductive hormones in infertile men. The Journal of Urology, 182(1):237-248.


24. Sh H. 2015. Effects of silver nanoparticles on sperm parameters, serum and seminal plasma reactive oxygen species. Journal of Cell and Tissue, 5(4):394-400.


25. Sharma A., Kshetrimayum C., Sadhu H.G., Kumar S. 2018. Arsenic-induced oxidative stress, cholinesterase activity in the brain of Swiss albino mice, and its amelioration by antioxidants Vitamin E and Coenzyme Q10. Environmental Science and Pollution Research, 25(24):23946-23953.


26. Shetty G., Wilson G., Huhtaniemi I., Boettger-Tong H., Meistrich M.L. 2001. Testosterone inhibits spermatogonial differentiation in juvenile spermatogonial depletion mice. Endocrinology, 142(7):2789-2795.


27. Swan S.H., Kruse R.L., Liu F., Barr D.B., Drobnis E.Z., Redmon J.B., Wang C., Brazil C., Overstreet J.W. 2003. Semen quality in relation to biomarkers of pesticide exposure. Environmental Health Perspectives, 111(12):1478-1484.


28. Tawfik M.K. 2015. Combination of coenzyme Q10 with methotrexate suppresses Freund's complete adjuvant-induced synovial inflammation with reduced hepatotoxicity in rats: Effect on oxidative stress and inflammation. International Immunopharmacology, 24(1): 80-87.


29. Xu, Y., Guo M., Jiang W., Dong H., Han Y., An X.F., Zhang J. 2016. Endoplasmic reticulum stress and its effects on renal tubular cells apoptosis in ischemic acute kidney injury. Renal Failure, 2016. 38(5):831-837.