The Effect of Bitter Olive Plant Extract (Melia azedarach) and Crocin on Mice with Hydatid Cyst

Subject Areas : Journal of Animal Biology

Raheleh Rahbarian ¹ , Shahrbanoo Jafari ² , Hamid Eshrati ³

1 - Department of Biology, Payame Noor University, Tehran, Iran
2 - Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
3 - Department of Biology, Payame Noor University, Tehran, Iran

Received: 2023-03-07 Accepted : 2023-05-08 Published : 2023-11-22

Keywords: hydatid cyst, Crocin, Biochemical factors, Melia azedarach,

Abstract :

Hydatid cyst is one of the most important common diseases between humans and animals. In the present study, the therapeutic effect of Melia azedarach seed extract and Crocin on a mouse model infected with hydatid cysts was investigated in vitro and in vivo conditions. In vitro study 0.1% eosin staining method was used. In vivo study, 30 female laboratory mice were infected with hydatid cysts by intraperitoneal injection of 0.5 ml of liquid containing 2000 protoscolex. One week after the injection, the mice were randomly divided into 5 treatment groups of 6 in separate cages. These groups include 1- control group (without any type of treatment), 2- Albendazole group (Albendazole dose 75 mg/kg), 3- Melia azedarach group (100 microliter Melia azedarach extract), 4- Crocin group (100 mg/ml crocin), 5- Melia azedarach - Crocin group (a mixture of Melia azedarach extract and Crocin). 140 days after the treatment, the desired tests were performed. In vitro investigation of the lethal effect of the pure Melia azedarach extract on protoscolexes showed that after 15 and 60 minutes of incubation, 60 and 80% of protoscolexes are destroyed, respectively. The results of in vivo tests also showed that in the groups treated with Crocin, Melia azedarach, and Melia azedarach-Crocin, the number, weight, and size of hydatid cysts were significantly reduced compared to the control. In addition, the examination of liver biochemical factors showed the most significant lethal effect of hydatid cyst in the groups treated with Melia azedarach and Melia azedarach-Crocin compared to the group treated with the anti-parasitic drug Albendazole and control.  

References:

1. Abdel-Ghany H.S.M., Abdel-Shafy S., Abuowarda , El-Khateeb R.M., Hoballah E.M., Fahmy M.M. 2021. Acaricidal activity of Artemisia herba-alba and Melia azedarach oil nanoemulsion against Hyalomma dromedarii and their toxicity on Swiss albino mice. Experimental and Applied Acarology, 84(1):241-262.


2. Al-Snafi A.E. 2016. The pharmacology of Crocus sativus-A review. IOSR Journal of Pharmacy, 6(6):8-38.


3. Balarastaghi S., Yazdian-Robati R., Vahdati Hasani F., Hosseinzadeh H., Abnous K., Imenshahidi M., Razavi B.M. 2021. Protective Effect of Crocin on Malathion-induced Cardiotoxicity in Rats: A Biochemical, Histopathological and Proteomics Study. Iranian Journal of Pharmaceutical Research, 20(2):156-172.


4. Gholami S., Rahimi-Esboei B., Ebrahimzadeh, M., Pourhajibagher M. 2013. In vitro effect of Sambucus ebulus on scolices of Hydatid cysts. European Review for Medical and Pharmacological Sciences, 17(13):1760-1765.


5. Hadjiakhoondi A., Vatandoost H., Khanavi M., Sadeghipour Roodsari H.R., Vosoughi M., Kazemi M., Abai M.R. 2006. Fatty Acid Composition and Toxicity of Melia azedarach L. Fruits against Malaria Vector Anopheles stephensi. Iranian Journal of Pharmaceutical Sciences, 2(2): 97-102.


6. Haji Mohammadi K.H., Heidarpour M., & Borji H. 2019. Allium Sativum Methanolic extract (garlic) improve therapeutic efficacy of albendazole against hydatid cyst: In vivo study. Journal of Investigative Surgery, 32(8):723-730.


7. Hussain R., Ihsan A., Shah A.A., Ullah N., Iftikhar H., Jalal R. 2022. Ecofriendly management of green pea (Pisum sativum) insect pests through plant extracts. Sarhad Journal of Agriculture, 38(4):1405-1411.‏


8. Hosseinzadeh H., Sadeghnia H.R., Ziaee, T., Danaee A. 2005. Protective effect of aqueous saffron extract (Crocus sativus L.) and crocin, its active constituent, on renal ischemia-reperfusion-induced oxidative damage in rats. Journal of Pharmacy and Pharmaceutical Sciences, 8(3):387-393.


9. Khoshraftar Z., Safekordi A., Shamel A., Zaefizadeh M. 2020. Evaluation of insecticidal activity of nanoformulation of Melia azedarach (leaf) extract as a safe environmental insecticide. International journal of environmental science and technology, 17(23):1159-1170.‏


10. Liu C., Fan H., Guan L., Ge R.L., Ma L. 2021. In vivo and in vitro efficacy of crocin against Echinococcus multilocularis. Parasites and Vectors, 14(1):1-17.


11. Mishra G., Jawla S., & Srivastava V. 2013. Melia azedarach: a review. International Journal of Medicinal Chemistry and Analysis, 3(2):53-56.


12. Moazeni M., Larki S., Saharkhiz M.J., Oryan A., Ansary Lari M., Mootabi Alavi A. 2014. In vivo study of the efficacy of the aromatic water of Zataria multiflora on hydatid cysts. Antimicrobial Agents and Chemotherapy, 58(10):6003-6008.


13. Moazeni M., Nazer A. 2010. In vitro effectiveness of garlic (Allium sativum) extract on scolices of hydatid cyst. World Journal of Surgery, 34(11):2677-2681.


14. Moazeni M., Saharkhiz M.J., Hosseini A.A. 2012. In vitro lethal effect of ajowan (Trachyspermum ammi L.) essential oil on hydatid cyst protoscoleces. Veterinary Parasitology, 187(1-2):203-208.


15. Mordue A.J., Nisbet A.J. 2000. Azadirachtin from the neem tree Azadirachta indica: its action against insects. Anais da Sociedade Entomológica do Brasil, 29:615-632.


16. Neogi N., Baliga, P., Srivastava R. 1963. In vitro Anthelmintic Activity of some Indigenous Drugs. Journal of the Indian Medical Association, 41(9):435-437.


17. Ntalli N.G., Cottiglia F., Bueno C.A., Alche L.E., Leonti M., Vargiu S., Caboni P. 2010. Cytotoxic Tirucallane Triterpenoids from Melia azedarach Fruits. Molecules, 15(9):5866-5877.


18. Omar M.K., Muhammad H.A., Mirkhan S.M. 2023. Effects of Crude Plant Extracts from Five Parts of Melia azedarach on Tribolium confusum. Aro-the Scientific Journal of Koya University, 11(1):48-51.‏


19. Peng Y., Gu T., Zhong T., Xiao,Y., Sun Q. 2022. Endoplasmic Reticulum Stress in Metabolic Disorders: Opposite Roles of Phytochemicals and Food Contaminants. Current Opinion in Food Science, 48:


20. Priyadarsini R.V., Murugan R.S., Sripriya P., Karunagaran D., Nagini S. 2010. The neem limonoids azadirachtin and nimbolide induce cell cycle arrest and mitochondria-mediated apoptosis in human cervical cancer (HeLa) cells. Free Radical Research, 44(6):624-634.


21. Ranjbar R., Shayanfar P., Maniati M. 2021. In Vitro Antileishmanial Effects of Saffron Compounds, Crocin and Stigmasterol, on Iranian Strain of Leishmania major (MHOM/IR/75/ER). Iranian Journal of Parasitology, 16(1):‏


22. Sariosseiri A., Moshaverinia A., Khodaparast M.H.H., Kalidari G.A. 2018. In vitro acaricidal effect of Melia azedarach ripe fruit extract against Dermanyssus gallinae (Acari: Dermanyssidae). Persian Journal of Acarology, 7(2):455.


23. Sayek I., Tirnaksiz M.B., & Dogan R. 2004. Cystic Hydatid Disease: Current Trends in Diagnosis and Management. Surgury Today, 34(12):987-996.


24. Sharma D., Paul Y. 2013. Preliminary and pharmacological profile of Melia azedarach L.: An overview. Journal of Applied Pharmaceutical Science, 3(12):133-138.


25. Sousa L.A.D.d., Rocha T.L., Sabóia-Morais S.M.T., Borges L.M.F. 2013. Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus) microplus treated with Melia azedarach. Revista Brasileira de Parasitologia Veterinária, 22(3):339-345.


26. Szewezuk V., Mongelli E.R., & Pomilio A.B. 2003. Antiparasitic activity of Melia azadirach growing in Argentina. Molecular Medicine and Chemistry, 1:54-57.


27. Thompson C.A. 2008. The taxonomy, phylogeny and transmission of Echinococcus. Experimental Parasitology, 119(4): 439-446.


28. Walker M., Rossignol J.F., Torgerson P., & Hemphill A. 2004. In vitro effects of nitazoxanide on Echinococcus granulosus protoscoleces and metacestodes. Journal of Antimicrobial Chemotherapy, 54(3):609-616.