Effect of Induced Obesity and Intense Interval Training on PI3K/AKT1/mTORc1 Axisin Cardiac Tissue of Male Wistar Rats

Subject Areas : Journal of Animal Biology

sina rezazadeh ¹ (Department of Exercise Physiology, Islamshahr Branch, Islamic Azad University, Tehran, Iran)
Sanaz Mirzayan Shanjani ² (Department of Exercise Physiology, Islamshahr Branch, Islamic Azad University, Tehran, Iran)
Mojtaba Eizadi ³ (Department of Exercise Physiology, Saveh Branch, Islamic Azad University, Saveh, Iran)
Saeed Sedaghati ⁴ (Department of Exercise Physiology, Islamshahr Branch, Islamic Azad University, Tehran, Iran)
Yaser Kazemzadeh ⁵ (Department of Exercise Physiology, Islamshahr Branch, Islamic Azad University, Tehran, Iran)

Received: 2023-12-18 Accepted : 2024-01-14 Published : 2024-03-05

Keywords: Obesity, Interval training, Gene expression, Physiological cardiac hypertrophy,

Abstract :

Epidemiological studies have always supported obesity as a cause of type 2 diabetes and cardiovascular diseases. The present study was conducted with the aim of determining the effect of intense interval exercise on the expression of some genes effective in physiological cardiac hypertrophy (PI3K, AKT1, mTORc1) in obese Wistar rats. For this purpose, from 21 male Wistar rats aged 10 weeks (220 ± 10 g), 14 after induction of obesity by 6 weeks of high-fat diet (HFD) were randomly divided to control obese (n = 7) or interval obese (n = 7) groups. Also, 7 rats with normal weight were selected as normal group. The interval obese rats were completed 8-weeks interval training (5 times weekly) in the form of interval runs on the treadmill. The control obese and normal groups did not participate in the exercise program. 48 hours after the last training session, the expression of PI3K, AKT1 and mTORc1 genes in heart tissue was measured and compared between groups by one-way ANOVA and Tukey's post hoc test. Induction of obesity led to a significant decrease in PI3K, AKT1 and mTORc1 in the heart tissue in the obese control group compared to the normal group (p = 0.001). Compared to the obese control group, interval training increased the expression of PI3K (p = 0.001) and mTORc1 (p = 0.001), but AKT1 expression did not change significantly in response to interval training (p = 0.603). Interval training is associated with improving the expression of genes affecting the physiological hypertrophy of the heart tissue in obese rats. Knowing the molecular cellular mechanisms responsible for this process requires more studies.

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