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Manuscript ID : ASCIJ-2302-1470 (R1) Visit : 288 Page: 113 - 130

10.22034/ascij.2023.1980337.1470

Article Type: Original Research

Investigating the Behavior of MG63 Cells on Polycaprolactone and Polycaprolactone/Collagen 3D Scaffolds for Bone Regeneration

Subject Areas : Journal of Animal Biology

Zahra Ebrahimi 1 , Shiva Irani 2 , Abdolreza Ardeshirylajimi 3 , Ehsan Seyedjafari 4

1 - Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4 - Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.

Received: 2023-01-16 Accepted : 2023-03-15 Published : 2023-11-22

Keywords: Collagen, stem cells, Bone tissue engineering, Polycaprolactone, 3D printing scaffolds,

Abstract :

3D printing of scaffolds at low temperatures is very promising for making artificial bone graft alternatives with more performance than traditional techniques. One of the most promising strategies in bone tissue engineering has focused on the development of biomimetic scaffolds. Ceramic-based scaffolds with osteogenic ability and mechanical properties are promising candidates for bone repair. The aim of this study was to adapt the flexibility and increasing the effect of inducing osteogenesis of Polycaprolactone (PCL) scaffold prepared by fused deposition modeling (FDM) method, using the combination of Collagen (COL) as a natural polymer with synthetic polymer and to investigate the behavior of MG63 cells on it. on it. After preparing the scaffold, scanning electron microscope (SEM), energy dispersive X-ray (EDX) and ATR-FTIR spectroscopy were used. After 1,7,14 days, the ossification process of MG63 cells in different treatments was performed using alizarin red staining and alkaline phosphatase activity. The non-toxicity of scaffolds was also evaluated by MTT assay to ensure cell proliferation. From under a microscope, it was found that tissue engineering scaffolds distributed and connected almost rectangular pores evenly. The PCL/COL scaffold showed a significant difference in terms of viability compared to the polycaprolactone scaffold only in the differential medium (P ≤ 0.0001). The results of evaluating ALP activity in PCL/COL scaffolds were significantly higher than uncoated PCL scaffolds and control (P ≤ 0.0001). The results of this study showed that the use of PCL/COL scaffold can be considered a suitable medium for proliferation and differentiation of MG63 cells in bone tissue engineering. Therefore, the PCL/COL composite scaffold prepared by FDM printer can be widely used in bone tissue engineering due to the cell survival by COL.

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