Investigating C23237T Polymorphism of Cyp3a4 Gene in Healthy People of West Mazandaran in Terms of Age and Gender

Subject Areas : Journal of Animal Biology

Fatemeh Heidarian ¹ (Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran)
Vida Hojati ² (Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran)
Reza Golijani Moghadam ³ (Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran)

Received: 2022-09-14 Accepted : 2023-01-08 Published : 2023-08-23

Keywords: gender, polymorphism, genotype, Cyp3a4, ARMS-PCR,

Abstract :

CYP3A4 gene is located on chromosome 7 and the largest number of substrates among cytochrome p450 enzymes belongs to it. The CYP3A4 gene is present in the endoplasmic reticulum of all tissues except the brain, although the main place of accumulation of its protein is in the liver and prostate. Because there have not been many studies on the polymorphisms of this gene, especially in Iran, this study was conducted in order to investigate the C23237T polymorphism of Cyp3a4 gene in 103 healthy male and female subjects aged 7 months to 79 years-old in the west of Mazandaran. In this research, whole blood was collected from healthy male and female individuals in the western cities of Mazandaran province. For each person, information related to age, sex, family history, alcohol and tobacco consumption, and the patient's medication were recorded. ARMS-PCR technique was used in this study. Data analysis was done using SPSS 2016 software and chi square test. The significant level of polymorphism relationship with gender and age of people was considered as p < 0.05. The results of the research showed that 70 people had the heterozygous status of the mutated AG genotype, the frequency of which was 67.96%. There was no significant relationship between genotype frequency and patients' age. Also, there is no significant relationship between C23237T polymorphism of Cype3a4 gene with age (p = 0.81) and gender (p = 0.845). According to the results of this research, it can be considered that people in any age group and with any gender have the same probability of contracting the disease.

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