• Home
  • Comparative Effect of Simvastatin with Methanolic Extract and Hyoscyamoside Extracted from Hyoscyamus niger Plant on APO A Gene Expression in Wistar Atherosclerosis Male Rats

Share To

Article Url


Manuscript ID : ASCIJ-2011-1214 (R1) Visit : 319 Page: 295 - 306

10.22034/ascij.2023.1916190.1214

Article Type: Original Research

Comparative Effect of Simvastatin with Methanolic Extract and Hyoscyamoside Extracted from Hyoscyamus niger Plant on APO A Gene Expression in Wistar Atherosclerosis Male Rats

Subject Areas : Journal of Animal Biology

Ronak Abdolmohammadi 1 , Maryam Bananej 2 , Maryam Khosravi 3 , Hengameh Alibeik 4

1 - Department Biology, North-Tehran Branch, Islamic Azad University, Tehran, Iran
2 - Department Biology, North-Tehran Branch, Islamic Azad University, Tehran, Iran
3 - Department Biology, North-Tehran Branch, Islamic Azad University, Tehran, Iran
4 - Department Biology, North-Tehran Branch, Islamic Azad University, Tehran, Iran

Received: 2022-09-14 Accepted : 2023-01-08 Published : 2023-08-23

Keywords: Atherosclerosis, serum lipids, Apo A gene, Contractile tone, Simvastatin, Hyoscyamoside,

Abstract :

Atherosclerosis is a chronic inflammatory disease of the artery that is mainly known by arterial wall thickening. The use of medicine herbs with minimal side effects in hypercholesterolemia treatment and removal of atherosclerotic plaque is important. The aim of the present study was to investigate the comparative effect of simvastatin with methanolic extract and hyoscyamoside extracted from Hyoscyamus niger plant on APO A gene expression in Wistar atherosclerosis male rats.Forty Wistar male rats weighing approximately 180 gr (at the beginning of the experiment) were prepared and randomly divided into 5 groups including 8 (control, sham, experimental 1, 2 and 3). All groups (except control) received fatty foods (1% cholesterol) in addition to 2% cholesterol for 40 days (receiving saline water 1 mg/kg daily by control group during this period). Then, the three experimental groups were treated daily with 40 mg/kg of simvastatin, 25 mg/kg of hyoscyamoside and 25 mg/kg of methanolic extract of Hyoscyamus niger plant for 28 days, respectively. During the treatment, sham group received 1 mg/kg saline water daily. Finally, APO A gene expression was determined by blood sampling with real-time PCR tecnique in leukocytes. Also, serum lipid levels were measured by photometric method. Methanolic extract and Hyoscyamus niger plant extracted-hyoscyamoside and simvastatin were caused a significant increase in APO A gene expression in all three experimental groups compared to sham group. Also, methanolic extract was more effective than other products in increasing this gene expression. Methanolic extract of Hyoscyamus niger plant and extracted hyoscyamoside have an additive effect on APO A gene expression and increase serum HDL levels. 

References:


  1. Ahmadi Y., Ghorbanihaghjo A., Argani H. 2017. The effect of statins on the organs: similar or contradictory? Journal of Cardiovascular and Thoracic Research, 9(2):64.
    2.
  2. Amna B., Uzma S., Umme H., Farha A., Ambreen M. 2016. Hyperlipidemia and hypertension; cardiovascular risk factors, various induction methods and their management by ethnomedicines. International Research Journal of Pharmacy, 7(11):1-9.
    3.
  3. Argmann C.A., Edwards J.Y., Sawyez C.G., O'Neil C.H., Hegele R.A., Pickering J.G., Huff M.W. 2005. Regulation of macrophage cholesterol efflux through hydroxymethylglutaryl-CoA reductase inhibition a role for RhoA in ABCA1-mediated Cholesterol efflux. Journal of Biological Chemistry, 280(23):22212-22221.
    4.
  4. Arinami T., Hirano T., Kobayashi K., Yamanouchi Y., Hamaguchi H. 1990. Assignment of the apolipoprotein AI gene to 11q23 based on RFLP in a case with a partial deletion of chromosome 11, del (11)(q23. 3→ qter). Human Genetics, 85(1):39-40.
    5.
  5. Aziz M., Yadav K.. 2016. Pathogenesis of atherosclerosis a review. Medical and Clinical Reviews, 2(3):1-6.
    6.
  6. Bachevski D., Damevska K., Simeonovski V., Dimova M., Back to the basics: Propolis and COVID‐19. Dermatologic Therapy, 33(4):e13780.
    7.
  7. Bandarian F., Hedayati M., Daneshpour M.S., Naseri M., Azizi F. 2013. Genetic polymorphisms in the APOA1 gene and their relationship with serum HDL cholesterol levels. Lipids, 48(12):1207-1216.
    8.
  8. Benhouda A., Yahia M., Benhouda D., Bousnane N., Benbia S., Hannachi N., Ghecham A. 2014. Antimicrobial and Antioxidant activities of various extracts of Hyoscyamus albus L. and Umbilicus rupestris L. leaves. Algerian Journal of Natural Products, 2:14-17.
    9.
  9. Bentzon J.F., Falk E. 2003. Pathology of stable and acute coronary syndromes. Theroux P, Ed Acute Coronary Syndromes: a Companion To Braunwald's Heart Disease: WB Saunders Company, p. 3327-3229.
    10.
  10. Boyd M., Tennant S., Melendez J., Toema D., Galen J., Geddes C., Levine M. 2015. Adaptation of red blood cell lysis represents a fundamental breakthrough that improves the sensitivity of S almonella detection in Journal of Applied Microbiology, 118(5):1199-1209.
    11.
  11. Cheraghi M., Asadi-Samani M. 2016. Atherosclerosis: Pathophysiology and promising herbal remedies in traditional Persian medicine. Der Pharmacia Lettre, 8(5):58-66.
    12.
  12. Diao S.-L., Sun J.-W., Ma B.-X., Li X.-M., Wang D. 2018. Influence of crocetin on high-cholesterol diet induced atherosclerosis in rats via anti-oxidant activity together with inhibition of inflammatory response and p38 MAPK signaling pathway. Saudi Journal of Biological Sciences, 25(3):493-499.
    13.
  13. Dod H.S., Bhardwaj R., Sajja V., Weidner G., Hobbs G.R., Konat G.W., Manivannan S., Gharib W., Warden B.E., Nanda N.C. 2010. Effect of intensive lifestyle changes on endothelial function and on inflammatory markers of atherosclerosis. The American Journal of Cardiology, 105(3):362-367.
    14.
  14. Dron J.S., Hegele R.A. 2016. Genetics of lipid and lipoprotein disorders and traits. Current genetic medicine reports, 4(3):130-141.
    15.
  15. El-Sayed -I.K., Al-Massarani S., El Gamal A., El-Shaibany A., Al-Mahbashi H.M., 2020. Mechanism of antidiabetic effects of Plicosepalus Acaciae flower in streptozotocin-induced type 2 diabetic rats, as complementary and alternative therapy. BMC Complementary Medicine and Therapies, 20(1):1-15.
    16.
  16. Hamidinia M., Boroujerdnia M.G., Talaiezadeh A., Solgi G., Roshani R., Iranprast S., Khodadadi A. 2015. Increased P-35, EBI3 transcripts and other treg markers in peripheral blood mononuclear cells of breast cancer patients with different clinical stages. Advanced Pharmaceutical Bulletin, 5(2):261.
    17.
  17. Herrington W., Lacey B., Sherliker P., Armitage J., Lewington S. 2016. Epidemiology of atherosclerosis and the potential to reduce the global burden of atherothrombotic disease. Circulation Research, 118(4):535-546.
    18.
  18. Inzucchi S.E., Bergenstal R.M., Buse J.B., Diamant M., Ferrannini E., Nauck M., Peters A.L., Tsapas A., Wender R., Matthews D.R. 2015. Management of hyperglycemia in type 2 diabetes, 2015: a patient-centered approach: update to a position statement of the American Diabetes Association and the European Association for the Study of Diabetes. Diabetes Care, 38(1):140-149.
    19.
  19. Karkhaneh L., Yaghmaei P., Parivar K., Sadeghizadeh M., Ebrahim-Habibi A. 2016. Effect of trans-chalcone on atheroma plaque formation, liver fibrosis and adiponectin gene expression in cholesterol-fed NMRI mice. Pharmacological Reports, 68(4):720-727.
    20.
  20. Keshetty V., Pabba S., Gudipati R., Kandukuri J.M., Allenki V. 2009. Antihyperlipidemic activity of methanolic extract of garlic (Allium sativum L.) in triton X-100 induced hyperlipidemic rats. Journal of Pharmaceutical Research, 2(5):23-31.
    21.
  21. Kishore S.P., Blank E., Heller D.J., Patel A., Peters A., Price M., Vidula M., Fuster V., Onuma O., Huffman M.D. 2018. Modernizing the World Health Organization list of essential medicines for preventing and controlling cardiovascular diseases. Journal of the American College of Cardiology, 71(5):564-574.
    22.
  22. Kosari M., Hasanpour M.R., Nuredini M., Safarpour Kachalpour E., Ahmadi R., Kosari M. 2013. Effect of methanol extract and hyoscyamuzides extracted hyoscyamous niger on blood pressure in Wistar rats. Journal of Nurse and Physition whitin War, 2013: 20-24.
    23.
  23. Lekar A., Borisenko S., Vetrova E., Sushkova S., Borisenko N., 2014. Extraction of quercetin from Polygonum hydropiper L. by subcritical water. American Journal of Agricultural and Biological Sciences, 9(1):1.
    24.
  24. Luo Y., Duan H., Qian Y., Feng L., Wu Z., Wang F., Feng J., Yang D., Qin Z., Yan X. 2017. Macrophagic CD146 promotes foam cell formation and retention during atherosclerosis. Cell Research, 27(3):352-372.
    25.
  25. Maguire E.M., Pearce S.W., Xiao Q. 2019. Foam cell formation: A new target for fighting atherosclerosis and cardiovascular disease. Vascular Pharmacology, 112:54-71.
    26.
  26. Mathur R.K. 2010. Role of diabetes, hypertension, and cigarette smoking on atherosclerosis. Journal of Cardiovascular Disease Research, 1(2):64-68.
    27.
  27. O'donnell M.J., Xavier D., Liu L., Zhang H., Chin S.L., Rao-Melacini P., Rangarajan S., Islam S., Pais P., McQueen M.J. 2010. Risk factors for ischaemic and intracerebral haemorrhagic stroke in 22 countries (the INTERSTROKE study): a case-control study. The Lancet, 376(9735):112-123.
    28.
  28. Patrono C., García Rodríguez L.A., Landolfi R., Baigent C. 2005. Low-dose aspirin for the prevention of atherothrombosis. New England Journal of Medicine, 353(22):2373-2383.
    29.
  29. Rodríguez-Saldaña J., Rodriguez-Flores M., Cantú-Brito C., Aguirre-Garcia J. 2014. A pathological study of the epidemiology of atherosclerosis in Mexico city. Cardiology Research and Practice, 2014.
    30.
  30. Su X.M., Wei Y., Wang Y., Zhang W. 2015. ABCA1 mRNA expression and cholesterol outflow in U937 cells. International journal of clinical and experimental pathology, 8(3):3116.
    31.
  31. Tripathi S., Srivastava S., Tripathi Y.B. 2019. Role of herbal nutraceuticals in prevention and treatment of atherosclerosis. Exploratory Animal and Medical Research, 9(1):15-23.
    32.
  32. Valledor A.F., Lloberas J., Celada A. 2015. Macrophage foam cells. eLS:1-10.
    33.
  33. Van der Vorst E.P. 2020. High-density Lipoproteins and Apolipoprotein A1. Vertebrate and Invertebrate Respiratory Proteins, Lipoproteins and other Body Fluid Proteins: Springer, p: 399-420.
    34.
  34. Verma P.K., Raina R., Sultana M., Singh M. 2016. Modulatory effect of Calendula officinalis on altered antioxidant status and renal parameters in diabetic rats. Pharmaceutical and Biomedical Research, 2(4):52-64.
    35.
  35. Veseli B.E., Perrotta P., De Meyer G.R., Roth L., Van der Donckt C., Martinet W., De Meyer G.R. 2017. Animal models of atherosclerosis. European Journal of pharmacology, 816:3-13.
    36.
  36. Yui Y., Aoyama T., Morishita H., Takahashi M., Takatsu Y., Kawai C. 1988. Serum prostacyclin stabilizing factor is identical to apolipoprotein AI (Apo AI). A novel function of Apo AI. The Journal of Clinical Investigation, 82(3):803-807.
    37.
  37. Zarei A., Ashtiyani S.C., Taheri S., Rasekh F. 2014. Comparison between effects of different doses of Melissa officinalis and atorvastatin on the activity of liver enzymes in hypercholesterolemia rats. Avicenna Journal of Phytomedicine, 4(1):15-23.
    38.
  38. Zhang W., Zhang W., Luo J., Kong L. 2013. A new steroidal glycoside from the seeds of Hyoscyamus niger. Natural Product Research, 27(21):1971-1974.
    39.

 

_||_

Related articles

The rights to this website are owned by the Raimag Press Management System.
Copyright © 2021-2024

Home| Login| About Us| Contact Us|
[فارسی] [العربية] [fa] [ar]