Investigating the Changes in Expression Level of FOXO1 and P27 Kip Genes in Endometrial Hyperplasia Tissue Samples Compared to Normal Tissue Sample

Subject Areas : Journal of Animal Biology

Parvin Ghaderi ¹ , Soodabeh Fallah ² , Hamid Reza Khaledi ³ , Afsaneh Tehranian ⁴ , Fereshteh Rahmati ⁵ , Shahrzad sheikhhasani ⁶

1 - Department of Biochemistry, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
3 - Department of Agricultural Engineering, Yadgar Imam Khomeini (RA) Shahrari Unit, Islamic Azad University, Tehran, Iran
4 - Department of Obstetrics and Gynecology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
5 - Department of Biochemistry, North Tehran Branch, Islamic Azad University, Tehran, Iran
6 - Department of Obstetrics and Gynecology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Received: 2022-09-14 Accepted : 2023-01-14 Published : 2023-11-22

Keywords: Endometrium, hyperplasia, FOXO1, P27 kip,

Abstract :

Endometrial hyperplasia is the irregular proliferation of endometrial glands and an increase in the ratio of gland to stroma compared to normal proliferative endometrium. Endometrial hyperplasia is one of the precursors of endometrial cancer, which is one of the most common cancers among women. The present study was conducted with the aim of investigating changes in the expression level of FOXO1 and P27 kip genes in endometrial hyperplasia tissue samples compared to normal tissue sample. In this case-control study, samples of hyperplasia with normal marginal tissue were taken from the uterine endometrium of women who referred to the women's clinic of Arash Hospital with abnormal uterine bleeding or increased endometrial thickness. Marginal normal tissue sample and hyperplasia sample were considered as control and case, respectively. The relative expression level of FOXO1 and P27 kip genes was measured by real time PCR method after total RNA extraction and cDNA synthesis, and then the data obtained from the reaction was analyzed by RQ=2-ΔΔCT method. Data were analyzed using independent t-test. The relative expression level of FOXO1 and P27 kip genes in the hyperplasia tissue sample was not significantly different compared to the control group (P>0.05.( Although the results of some studies indicate that the expression level of FOXO1 and P27KIP genes can be changed in endometrial cancer, the results of the present study showed that, contrary to expectations, there is no significant change in relative expression level of FOXO1 and P27KIP genes in hyperplasia tissue compared to normal tissue.

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