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Manuscript ID : ASCIJ-2301-1461 (R1) Visit : 406 Page: 133 - 146

10.22034/ascij.2023.1978481.1461

Article Type: Original Research

The Effect of Intraperitoneal Injection of Carvacrol Administration on Lipopolysaccharides-Induced Renal Oxidative Stress in Male Rats

Subject Areas : Journal of Animal Biology

Alireza Mortazavi 1 , Hossain Mohammad Pourkargar 2 , Farimah Beheshti 3 , Gholamhasan Vaezi 4 , Mahmoud Hosseini 5

1 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
2 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
3 - Neuroscience Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran|Department of Physiology, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
4 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
5 - Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Received: 2022-09-14 Accepted : 2023-01-08 Published : 2023-08-23

Keywords: Carvacrol, Oxidative stress, Lipopolysaccharide, IL-1β, Acute Kidney Injury,

Abstract :

Carvacrol is a natural monoterpene phenol which retains significant antibacterial, antifungal, anti-cancer and anti-inflammatory activity. However, the effect of carvacrol on oxidative stress in lipopolysaccharide-induced acute kidney injury has not yet been reported. The present study aimed to investigate the protective effects of carvacrol on lipopolysaccharides-induced renal oxidative stress and inflammatory responses in male rats. Male Wistar rats (weighing 200 to 250 g, n=7 each group) were divided into the following groups: 1) control, 2) LPS, 3) LPS+carvacrol 25 mg/kg, 4) LPS+carvacrol 50 mg/kg and 5) LPS+carvacrol 100 mg/kg. For inducing acute kidney injury, 1 mg/kg LPS was injected intraperitoneally for 2 weeks. Carvacrol was administered intraperitoneally 30 minutes prior LPS injection. After preparation of renal homogenates, levels of inflammatory cytokines IL-1β and oxidative stress indexes (malondialdehyde, total thiol, catalase and Superoxide dismutase activity) were detected by ELISA and spectrophotometric methods, respectively. Our results showed that LPS injection increased malondialdehyde and IL-1β while, it reduced total thiol, catalase and SOD activity in the renal tissue. But, pretreatment with carvacrol not only decreased malondialdehyde and IL-1β levels but also increased total thiol, catalase and SOD activity. Simple regression analysis revealed that MDA was positively correlated with IL-1β level). However, there was a significant negative correlation between MDA level and total thiol, SOD and CAT activities. Our results showed that carvacrol protects the kidney by reducing IL-1β and preventing oxidative stress in the renal tissue.enal tissue.

References:


  1. Aragno M., Cutrin J.C., Mastrocola R., Perrelli M-G, Restivo F, Poli G, et al. 2003. Oxidative stress and kidney dysfunction due to ischemia/reperfusion in rat: attenuation by dehydroepiandrosterone. Kidney International, 64(3):836-843.
    2.
  2. Aydin Y, Kutlay Ö, Ari S, Duman S, Uzuner K, Aydin S. 2007. Hypotensive effects of carvacrol on the blood pressure of normotensive rats. Planta medica, 73(13):1365-1371.
    3.
  3. Baylis C, Mitruka B, Deng A. 1992. Chronic blockade of nitric oxide synthesis in the rat produces systemic hypertension and glomerular damage. The Journal of clinical investigation, 90(1):278-81.
    4.
  4. Bogdan C. 2001. Nitric oxide and the immune response. Nature Immunology, 2(10):907-916.
    5.
  5. Bussolati B, David S, Cambi V, Tobias PS, Camussi G. 2002. Urinary soluble CD14 mediates human proximal tubular epithelial cell injury induced by LPS. International Journal of Molecular Medicine, 10(4):441-449.
    6.
  6. Chen Y, Jin S, Teng X, Hu Z, Zhang Z, Qiu X, et al. 2018. Hydrogen sulfide attenuates LPS-induced acute kidney injury by inhibiting inflammation and oxidative stress. Oxidative Medicine and Cellular Longevity, 2018: 6717212.
    7.
  7. Faas M, Schuiling G, Valkhof N, Baller J, Bakker W. 1998. Superoxide–Mediated Glomerulopathy in the Endotoxin–Treated Pregnant Rat. Kidney and Blood Pressure Research, 21(6):432-437.
    8.
  8. Fl Lin J, Lee T-S, Kou YR, Tarng D-C. 2021. Role of TRPA1 in Tissue Damage and Kidney Disease. International Journal of Molecular Sciences, 22(7):3415.
    9.
  9. Fu H, Hu Z, Di X, Zhang Q, Zhou R, Du H. 2016. Tenuigenin exhibits protective effects against LPS-induced acute kidney injury via inhibiting TLR4/NF-κB signaling pathway. European Journal of Pharmacology, 791:229-234.
    10.
  10. Gardiner SM, Kemp PA, March JE, Bennett T. 1999. Influence of FR 167653, an inhibitor of TNF-alpha and IL-1, on the cardiovascular responses to chronic infusion of lipopolysaccharide in conscious rats. Journal of Cardiovascular Pharmacology, 34(1):64-69.
    11.
  11. Gill R, Tsung A, Billiar T. 2010. Linking oxidative stress to inflammation: Toll-like receptors. Free Radical Biology and Medicine, 48(9):1121-32.
    12.
  12. Goodman CW, Brett AS. 2017. Gabapentin and pregabalin for pain—is increased prescribing a cause for concern? New England Journal of Medicine, 377(5):411-4.
    13.
  13. Hakimi Z, Salmani H, Marefati N, Arab Z, Gholamnezhad Z, Beheshti F, et al. 2020. Protective Effects of Carvacrol on Brain Tissue Inflammation and Oxidative Stress as well as Learning and Memory in Lipopolysaccharide-Challenged Rats. Neurotox Research, 37(4):965-976.
    14.
  14. Hewett JA, Roth RA. 1993. Hepatic and extrahepatic pathobiology of bacterial lipopolysaccharides. Pharmacological Reviews, 45(4):381-411.
    15.
  15. Hosseini M, Beheshti F, Anaeigoudari A. 2020, Improving Effect of Aminoguanidine on Lipopolysaccharide-Caused Kidney Dysfunction in Rats. Saudi Journal of Kidney Diseases and Transplantation, 31(5):1025-1033.
    16.
  16. Hsu SP, Chen CC, Chien CT. 2016. Pretreatment of Sialic Acid Efficiently Prevents Lipopolysaccharide-Induced Acute Renal Failure and Suppresses TLR4/gp91-Mediated Apoptotic Signaling. Kidney and Blood Pressure Research, 41(3):267-277.
    17.
  17. Ilçe F, Gök G, Pandir D. 2019. Acute effects of lipopolysaccharide (LPS) in kidney of rats and preventive role of vitamin E and sodium selenite. Hum Exp Toxicol, 38(5):547-560.
    18.
  18. Jansen A, Cook T, Taylor GM, Largen P, Riveros-Moreno V, Moncada S, et al. 1994. Induction of nitric oxide synthase in rat immune complex glomerulonephritis. Kidney International, 45(4):1215-9.
    19.
  19. Khalil A, Kovac S, Morris G, Walker MC. 2017. Carvacrol after status epilepticus (SE) prevents recurrent SE, early seizures, cell death, and cognitive decline. Epilepsia, 58(2):263-73.
    20.
  20. Lee B, Yeom M, Shim I, Lee H, Hahm DH. 2020. Inhibitory effect of carvacrol on lipopolysaccharide-induced memory impairment in rats. The Korean journal of physiology and Pharmacology, 24(1):27-37.
    21.
  21. Liu M, Bing G. 2011. Lipopolysaccharide animal models for Parkinson's disease. Parkinson's Disease, 11:327089.
    22.
  22. Liu S, Song M, Yun W, Lee J, Kim H, Cho J. 2019. Effect of carvacrol essential oils on immune response and inflammation-related genes expression in broilers challenged by lipopolysaccharide. Poultry science, 98(5):2026-33.
    23.
  23. Malis C, Bonventre J. 1986. Mechanism of calcium potentiation of oxygen free radical injury to renal mitochondria. A model for post-ischemic and toxic mitochondrial damage. Journal of Biological Chemistry, 261(30):14201-8.
    24.
  24. Martı́nez M, Hernández AI, Martı́nez N. 2000. N-Acetylcysteine delays age-associated memory impairment in mice: role in synaptic mitochondria. Brain Research, 855(1):100-106.
    25.
  25. Mir SM, Ravuri HG, Pradhan RK, Narra S, Kumar JM, Kuncha M, et al. 2018. Ferulic acid protects lipopolysaccharide-induced acute kidney injury by suppressing inflammatory events and upregulating antioxidant defenses in Balb/c mice. Biomedicine and Pharmacotherapy, 100:304-315.
    26.
  26. Mortazavi A, Mohammad Pour Kargar H, Beheshti F, Anaeigoudari A, Vaezi G, Hosseini M. 2021. The effects of carvacrol on oxidative stress, inflammation, and liver function indicators in a systemic inflammation model induced by lipopolysaccharide in rats. International Journal for Vitamin and Nutrition Research, 1-11.
    27.
  27. Ngkelo A, Meja K, Yeadon M, Adcock I, Kirkham PA. 2012. LPS induced inflammatory responses in human peripheral blood mononuclear cells is mediated through NOX4 and Giα dependent PI-3kinase signalling. Journal of Inflammation (London, England), 9(1):1.
    28.
  28. Ohkawa H, Ohishi N, Yagi K. 1978. Reaction of linoleic acid hydroperoxide with thiobarbituric acid. Journal of Lipid Research, 19(8):1053-7.
    29.
  29. Yi Li and Schell h. 2007. Rapid kinetic microassay for catalase activity. Journal of biomolecular techniques, 19(8):185-7.
    30.
  30. Ozer EK, Goktas MT, Toker A, Bariskaner H, Ugurluoglu C, Iskit AB. 2017. Effects of carvacrol on survival, mesenteric blood flow, aortic function and multiple organ injury in a murine model of polymicrobial sepsis. Inflammation, 40(5):1654-63.
    31.
  31. Salmaninejad A, Kangari P, Shakoori A. 2017. Oxidative stress: development and progression of breast cancer. Tehran University Medical Journal TUMS Publications, 75(1):1-9.
    32.
  32. Samarghandian S, Farkhondeh T, Samini F, Borji A. 2016. Protective Effects of Carvacrol against Oxidative Stress Induced by Chronic Stress in Rat's Brain, Liver, and Kidney. Biochemistry Research International, 2016:2645237.
    33.
  33. Schor N. Acute renal failure and the sepsis syndrome. 2002. Kidney International, 61(2):764-76.
    34.
  34. Shum H-P, Yan W-W, Chan TM. 2016. Recent knowledge on the pathophysiology of septic acute kidney injury: a narrative review. Journal of Critical Care, 31(1):82-9.
    35.
  35. Suntres ZE, Coccimiglio J, Alipour M. 2015. The bioactivity and toxicological actions of carvacrol. Critical Reviews in Food Science and Nutrition, 55(3):304-18.
    36.
  36. Tepe B, Sokmen M, Akpulat HA, Daferera D, Polissiou M, Sokmen A. 2005. Antioxidative activity of the essential oils of Thymus sipyleus subsp. sipyleus var. sipyleus and Thymus sipyleus subsp. sipyleus var. rosulans. Journal of Food Engineering, 66(4):447-54.
    37.
  37. Uyanoglu M, Canbek M, Ceyhan E, Senturk H, Bayramoglu G, Gunduz O, et al. 2011. Preventing organ injury with carvacrol after renal ischemia/reperfusion. Journal of Medicinal Plants Research, 5(1):72-80.
    38.
  38. White SB. 2011. Antibacterial efficacy of phosvitin, carvacrol, or nisin alone or combined against foodborne human enteric pathogens. Chapter 1:72-80.
    39.
  39. Wu C-K, Lin J-F, Lee T-S, Kou YR, Tarng D-C. 2021. Role of TRPA1 in Tissue Damage and Kidney Disease. International Journal of Molecular Sciences, 22(7):3415.
    40.
  40. Yuan H, Perry CN, Huang C, Iwai-Kanai E, Carreira RS, Glembotski CC, et al. 2009. LPS-induced autophagy is mediated by oxidative signaling in cardiomyocytes and is associated with cytoprotection. American Journal of Physiology-Heart and Circulatory Physiology, 296(2):470-479.
    41.
  41. Zhang C, Walker LM, Mayeux PR. 2000. Role of nitric oxide in lipopolysaccharide-induced oxidant stress in the rat kidney. Biochemical Pharmacology, 59(2):203):203-209.
    42.
  42. Zhu J, Zhang S, Geng Y, Song Y. 2018. Transient receptor potential ankyrin 1 protects against sepsis-induced kidney injury by modulating mitochondrial biogenesis and mitophagy. American Journal of Translational Research, 10(12):4163.
    43.

 

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